Detailed information of regulator


Summary

Regulator ID REG0058 experimental
Regulator type protein
Regulatory pathway (from literatures) Unknown
KEGG pathway (determined by BlastKOALA) RNA degradation (map03018)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (1058 a.a.); Nucleotide sequence (3174 bp)
Description RNase E plays a critical role in repressing icmF1 associated with the T6SS.
Reference
External database links

Locus tag (Gene) PA2976 (RNase E)
Coordinates (Strand) 3332881..3336054 (+)
NCBI ID 15598172
RefSeq NC_002516
Uniprot ID Q9HZM8_PSEAE
KEGG ID pae:PA2976
KEGG pathway (determined by BlastKOALA) map03018
PDB ID 5FT1
  Target
Target Regulation (↓/↑) Target sequence
PA0077 (icmF1) expression ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
RNase_E_G PF10150.11 1.2e-95 119..388
S1 PF00575.25 6.8e-15 36..116
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1057 MKRMLINATQPEELRVALVDGQRLFDLDIESGAREQKKANIYKGRITRVEPSLEAAFVDFGAERHGFLPLKEISREYFKKSPEGRINIKEVLSEGQEVIVQVEKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLNAPADMGLIVRTAGLGRSTEELQWDLDYLLQLWSAIKEASGERGAPFLIYQESNVIIRAIRDYLRQDIGEVLIDSIDAQEEALNFIRQVMPQYASKVKLYQDSVPLFNRFQIESQIETAFQREVKLPSGGSIVIDPTEALVSIDINSARATKGGDIEETALQTNLEAAEEIARQLRLRDIGGLIVIDFIDMTPAKNQRAVEERVREALEADRARVQVGRISRFGLLEMSRQRLRPSLGETSGIVCPRCNGQGIIRDVESLSLAILRLIEEEALKDRTAEVRARVPFQVAAFLLNEKRNAITKIELRTRARIFILPDDHLETPHFEVQRLRDDSPELVAGQTSYEMATVEHEEAQPVSSTRTLVRQEAAVKTVAPQQPAPQHTEAPVEPAKPMPEPSLFQGLVKSLVGLFAGKDQPAAKPAETSKPAAERQTRQDERRNGRQQNRRRDGRDGNRRDEERKPREERAERQPREERAERPNREERSERRREERAERPAREERQPREGREERAERTPREERQPREGREGREERSERRREERAERPAREERQPREGREERAERPAREERQPREDRQARDAAALEAEALPNDESLEQDEQDDTDGERPRRRSRGQRRRSNRRERQREVSGELEGSEATDNAAAPLNTVAAAAAAGIAVASEAVEANVEQAPATTSEAASETTASDETDASTSEAVETQGADSEANTGETADIEAPVTVSVVRDEADQSTLLVAQATEEAPFASESVESREDAESAVQPATEAAEEVAAPVPVEVAAPSEPAATEEPTPAIAAVPANATGRALNDPREKRRLQREAERLAREAAAAAEAAAQAAPAVEEIPAVASEEASAQEEPAAPQAEEITQADVPSQADEAQEAVQAEPEASGEGAADTEHAKKTEESETSRPHA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9927 - -


  Protein sequence of regulator: 1058 a.a.    .

>REG0058 NC_002516:3332881-3336054 [Pseudomonas aeruginosa PAO1]
MKRMLINATQPEELRVALVDGQRLFDLDIESGAREQKKANIYKGRITRVEPSLEAAFVDFGAERHGFLPLKEISREYFKK
SPEGRINIKEVLSEGQEVIVQVEKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLNAPA
DMGLIVRTAGLGRSTEELQWDLDYLLQLWSAIKEASGERGAPFLIYQESNVIIRAIRDYLRQDIGEVLIDSIDAQEEALN
FIRQVMPQYASKVKLYQDSVPLFNRFQIESQIETAFQREVKLPSGGSIVIDPTEALVSIDINSARATKGGDIEETALQTN
LEAAEEIARQLRLRDIGGLIVIDFIDMTPAKNQRAVEERVREALEADRARVQVGRISRFGLLEMSRQRLRPSLGETSGIV
CPRCNGQGIIRDVESLSLAILRLIEEEALKDRTAEVRARVPFQVAAFLLNEKRNAITKIELRTRARIFILPDDHLETPHF
EVQRLRDDSPELVAGQTSYEMATVEHEEAQPVSSTRTLVRQEAAVKTVAPQQPAPQHTEAPVEPAKPMPEPSLFQGLVKS
LVGLFAGKDQPAAKPAETSKPAAERQTRQDERRNGRQQNRRRDGRDGNRRDEERKPREERAERQPREERAERPNREERSE
RRREERAERPAREERQPREGREERAERTPREERQPREGREGREERSERRREERAERPAREERQPREGREERAERPAREER
QPREDRQARDAAALEAEALPNDESLEQDEQDDTDGERPRRRSRGQRRRSNRRERQREVSGELEGSEATDNAAAPLNTVAA
AAAAGIAVASEAVEANVEQAPATTSEAASETTASDETDASTSEAVETQGADSEANTGETADIEAPVTVSVVRDEADQSTL
LVAQATEEAPFASESVESREDAESAVQPATEAAEEVAAPVPVEVAAPSEPAATEEPTPAIAAVPANATGRALNDPREKRR
LQREAERLAREAAAAAEAAAQAAPAVEEIPAVASEEASAQEEPAAPQAEEITQADVPSQADEAQEAVQAEPEASGEGAAD
TEHAKKTEESETSRPHA*

  Nucleotide sequence of regulator: 3174 bp    .

>REG0058 NC_002516:3332881-3336054 [Pseudomonas aeruginosa PAO1]
ATGAAAAGAATGCTGATCAACGCGACTCAACCCGAGGAGTTGCGTGTTGCACTGGTCGACGGCCAACGCCTGTTCGACCT
CGACATCGAGTCGGGCGCCCGCGAGCAGAAGAAGGCCAACATCTACAAAGGCCGCATCACTCGCGTCGAACCCAGCCTCG
AAGCCGCCTTCGTCGACTTCGGCGCCGAACGCCACGGCTTCCTCCCCCTCAAAGAAATCTCCCGCGAATACTTCAAGAAA
TCCCCCGAAGGCCGGATCAACATCAAGGAAGTCCTGAGCGAAGGCCAGGAAGTCATCGTCCAGGTCGAGAAGGAAGAGCG
CGGCAACAAGGGCGCCGCCCTGACCACCTTCATCAGCCTGGCCGGCCGTTACCTGGTGCTGATGCCGAACAACCCGCGCG
CCGGCGGCATCTCCCGCCGTATCGAAGGCGAAGAGCGCAACGAGCTGCGCGAGGCCCTGAACGGCCTCAACGCACCGGCC
GACATGGGCCTGATCGTGCGCACCGCCGGCCTCGGCCGCAGCACCGAGGAACTGCAGTGGGACCTCGACTACCTGCTGCA
ACTGTGGAGCGCGATCAAGGAAGCGTCCGGCGAACGTGGCGCGCCCTTCCTGATCTACCAGGAAAGCAACGTCATCATCC
GCGCCATCCGCGACTACCTGCGCCAGGACATCGGCGAAGTGCTGATCGACAGCATCGACGCCCAGGAAGAAGCCCTGAAC
TTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTGAAGCTGTACCAGGACAGCGTTCCGCTGTTCAATCGCTTCCA
GATCGAGAGCCAGATCGAGACCGCTTTCCAGCGCGAAGTGAAGCTGCCGTCCGGCGGCTCCATCGTCATCGACCCGACCG
AGGCCCTGGTTTCCATCGACATCAACTCGGCGCGCGCCACCAAGGGCGGCGACATCGAGGAAACCGCCCTGCAGACCAAC
CTGGAAGCGGCCGAGGAAATCGCCCGCCAGCTGCGCCTGCGTGACATCGGCGGCCTGATCGTCATCGACTTCATCGACAT
GACCCCGGCGAAGAACCAGCGCGCCGTGGAAGAGCGTGTCCGCGAAGCCCTCGAGGCCGACCGCGCGCGCGTCCAGGTCG
GTCGCATCTCGCGCTTCGGCCTGCTGGAAATGTCCCGCCAGCGCCTGCGTCCGTCCCTCGGCGAGACCAGCGGCATCGTC
TGCCCGCGCTGCAACGGCCAGGGCATCATCCGCGACGTCGAGTCGCTGTCGCTGGCCATCCTGCGCCTGATCGAGGAAGA
AGCCCTGAAGGACCGCACCGCGGAAGTCCGCGCCCGCGTGCCCTTCCAGGTCGCCGCCTTCCTGCTCAACGAGAAGCGCA
ACGCCATCACCAAGATCGAACTGCGTACCCGCGCGCGCATCTTCATCCTGCCGGACGATCATCTGGAAACCCCGCATTTC
GAAGTCCAGCGTCTGCGCGATGACAGCCCCGAACTGGTTGCCGGCCAGACCAGCTACGAAATGGCCACCGTCGAGCACGA
AGAAGCCCAGCCGGTCAGCTCGACCCGCACCCTGGTCCGCCAGGAAGCAGCAGTCAAGACCGTCGCTCCCCAGCAGCCCG
CACCGCAACACACCGAAGCACCGGTCGAGCCGGCCAAGCCGATGCCCGAGCCGAGCCTGTTCCAGGGCCTGGTGAAGTCC
CTGGTCGGCCTGTTCGCAGGCAAGGATCAACCTGCCGCCAAGCCTGCTGAAACCAGCAAGCCGGCTGCCGAGCGCCAAAC
CCGCCAGGACGAGCGTCGCAACGGCCGCCAGCAGAACCGCCGCCGCGATGGCCGCGATGGCAATCGCCGCGACGAAGAGC
GCAAACCGCGCGAGGAGCGTGCAGAGCGTCAACCGCGCGAAGAGCGCGCCGAACGCCCGAACCGCGAAGAGCGCAGCGAA
CGTCGCCGCGAAGAGCGCGCCGAGCGCCCGGCTCGCGAGGAGCGCCAGCCGCGCGAAGGCCGTGAAGAGCGCGCCGAGCG
GACACCCCGCGAAGAGCGCCAGCCGCGCGAAGGCCGCGAAGGTCGCGAGGAACGCAGCGAACGCCGCCGCGAAGAGCGCG
CCGAACGCCCGGCCCGCGAAGAGCGCCAACCCCGCGAAGGCCGTGAAGAACGCGCCGAGCGCCCGGCCCGCGAAGAGCGC
CAGCCGCGCGAGGATCGCCAGGCTCGCGACGCCGCGGCCCTGGAAGCCGAGGCATTGCCGAACGACGAGAGCCTGGAGCA
GGACGAGCAGGACGATACCGATGGCGAGCGCCCGCGCCGCCGCTCCCGCGGCCAGCGTCGTCGCAGCAACCGCCGCGAAC
GCCAGCGCGAGGTCAGCGGCGAGCTGGAAGGCAGCGAGGCGACCGATAACGCCGCCGCGCCGCTGAACACCGTCGCAGCC
GCCGCCGCTGCCGGTATCGCTGTCGCCAGCGAAGCCGTAGAAGCAAACGTGGAGCAAGCCCCGGCCACTACCAGCGAGGC
TGCCAGCGAAACCACGGCAAGCGATGAGACCGACGCGTCGACCAGCGAAGCCGTCGAAACCCAGGGCGCGGACAGCGAGG
CCAATACCGGCGAAACCGCCGACATCGAAGCGCCGGTGACCGTCAGCGTGGTCCGGGACGAAGCCGACCAGAGCACCCTG
CTGGTCGCGCAAGCCACTGAAGAAGCTCCCTTCGCCAGCGAAAGCGTGGAAAGCCGCGAAGACGCCGAGAGCGCCGTGCA
ACCGGCAACGGAAGCGGCCGAAGAAGTTGCCGCTCCGGTGCCCGTCGAAGTAGCAGCCCCTAGCGAGCCCGCAGCCACCG
AGGAGCCGACCCCGGCCATCGCGGCGGTGCCGGCCAACGCGACTGGCCGTGCCCTCAACGACCCACGGGAAAAACGTCGC
CTGCAACGCGAAGCCGAGCGTCTGGCACGCGAAGCCGCAGCAGCAGCCGAAGCGGCAGCTCAGGCCGCTCCCGCCGTCGA
GGAGATCCCGGCTGTAGCGAGCGAGGAAGCGTCGGCCCAGGAGGAACCTGCTGCACCCCAGGCTGAAGAAATCACCCAGG
CCGACGTTCCGTCCCAGGCGGACGAAGCCCAGGAAGCGGTACAGGCCGAGCCTGAAGCTTCCGGCGAAGGCGCCGCCGAC
ACGGAGCACGCGAAAAAGACCGAGGAAAGCGAAACCTCGCGCCCGCATGCCTGA