Detailed information of regulator
Summary
| Regulator ID | REG0050  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Unknown |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (348 a.a.); Nucleotide sequence (1044 bp) |
| Description | The expression of hcp1, hcp2, hcp3 (effector secreted proteins) and clpV1 (T6S ATPase) was tested by real‐time RT‐PCR at mid‐logarithmic phase and transcriptomic analysis confirmed the repressed state of T6SS by an active MexT. |
| Reference |
External database links
| Locus tag (Gene) | PA2492 (MexT) |
| Coordinates (Strand) | 2807469..2808512 (+) |
| NCBI ID | 15597688 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I0Z0_PSEAE |
| KEGG ID | pae:PA2492 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 6L33 |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS transcripts | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| LysR_substrate | PF03466.22 | 3.1e-19 | 144..335 |
| HTH_1 | PF00126.29 | 3.4e-07 | 80..113 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-347 | MPVSDPMPLRHLARPRPVSHARLDGEPPRLQPLAPGNEERHEPKRPAPRRSEPADRVRDPDARTQRDPRRRETVPRPAGQPAISAALSRLRTLFDDPLFVRTGRSMEPTARAQEIFAHLSPALDSISTAMSRASEFDPATSTAVFRIGLSDDVEFGLLPPLLRRLRAEAPGFVLVVRRANYLLMPNLLASGEISVGVSYTDELPANAKRKTVRRSKPKILRADSAPGQLTLDDYCARPHALVSFAGDLSGFVDEELEKFGRKRKVVLAVPQFNGLGTLLAGTDIIATVPDYAAQALIAAGGLRAEDPPFETRAFELSMAWRGAQDNDPAERWLRSRISMFIGDPDSL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9453 | - | - |
Protein sequence of regulator: 348 a.a. .
>REG0050 NC_002516:2807469-2808512 [Pseudomonas aeruginosa PAO1]
MPVSDPMPLRHLARPRPVSHARLDGEPPRLQPLAPGNEERHEPKRPAPRRSEPADRVRDPDARTQRDPRRRETVPRPAGQ
PAISAALSRLRTLFDDPLFVRTGRSMEPTARAQEIFAHLSPALDSISTAMSRASEFDPATSTAVFRIGLSDDVEFGLLPP
LLRRLRAEAPGFVLVVRRANYLLMPNLLASGEISVGVSYTDELPANAKRKTVRRSKPKILRADSAPGQLTLDDYCARPHA
LVSFAGDLSGFVDEELEKFGRKRKVVLAVPQFNGLGTLLAGTDIIATVPDYAAQALIAAGGLRAEDPPFETRAFELSMAW
RGAQDNDPAERWLRSRISMFIGDPDSL*
MPVSDPMPLRHLARPRPVSHARLDGEPPRLQPLAPGNEERHEPKRPAPRRSEPADRVRDPDARTQRDPRRRETVPRPAGQ
PAISAALSRLRTLFDDPLFVRTGRSMEPTARAQEIFAHLSPALDSISTAMSRASEFDPATSTAVFRIGLSDDVEFGLLPP
LLRRLRAEAPGFVLVVRRANYLLMPNLLASGEISVGVSYTDELPANAKRKTVRRSKPKILRADSAPGQLTLDDYCARPHA
LVSFAGDLSGFVDEELEKFGRKRKVVLAVPQFNGLGTLLAGTDIIATVPDYAAQALIAAGGLRAEDPPFETRAFELSMAW
RGAQDNDPAERWLRSRISMFIGDPDSL*
Nucleotide sequence of regulator: 1044 bp .
>REG0050 NC_002516:2807469-2808512 [Pseudomonas aeruginosa PAO1]
ATGCCTGTCAGTGATCCTATGCCCCTCCGGCACCTCGCCAGGCCCCGCCCCGTCTCGCACGCAAGGCTTGACGGCGAGCC
CCCGCGGTTGCAGCCTCTAGCCCCTGGAAACGAGGAACGCCATGAACCGAAACGACCTGCGCCGCGTCGATCTGAACCTG
CTGATCGTGTTCGAGACCCTGATGCACGAACGCAGCGTGACCCGCGCCGCAGAGAAACTGTTCCTCGGCCAGCCGGCCAG
CCGGCCATCAGCGCCGCGCTGTCGCGCCTGCGCACGCTGTTCGACGACCCGCTGTTCGTCCGTACCGGACGCAGCATGGA
GCCCACCGCGCGAGCCCAGGAAATCTTCGCCCACCTGTCGCCGGCGCTGGATTCCATCTCCACCGCCATGAGTCGCGCCA
GCGAGTTCGATCCGGCGACCAGCACCGCGGTGTTCCGCATCGGCCTTTCCGACGACGTCGAGTTCGGCCTGTTGCCGCCC
CTGCTCCGCCGCCTGCGCGCGGAGGCGCCGGGGTTCGTCCTCGTCGTGCGCCGCGCCAACTATCTATTGATGCCGAACCT
GCTGGCCTCGGGGGAGATCTCGGTGGGCGTCAGCTACACCGACGAACTGCCGGCCAACGCCAAGCGCAAGACCGTGCGCC
GCAGCAAGCCGAAGATCCTCCGCGCCGACTCCGCGCCCGGCCAGCTGACCCTCGACGACTATTGCGCGCGACCGCACGCG
CTGGTGTCCTTCGCCGGCGACCTCAGCGGCTTCGTCGACGAGGAGCTGGAAAAATTCGGCCGCAAGCGCAAGGTGGTCCT
GGCGGTGCCGCAGTTCAACGGCCTCGGCACCCTCCTGGCCGGCACCGACATCATCGCCACCGTGCCCGACTACGCCGCCC
AGGCGCTGATCGCCGCCGGCGGCCTACGCGCCGAGGACCCACCGTTCGAGACCCGGGCCTTCGAACTGTCGATGGCTTGG
CGCGGCGCCCAGGACAACGATCCGGCCGAACGCTGGCTGCGCTCGCGGATCAGCATGTTCATCGGCGATCCGGACAGTCT
CTGA
ATGCCTGTCAGTGATCCTATGCCCCTCCGGCACCTCGCCAGGCCCCGCCCCGTCTCGCACGCAAGGCTTGACGGCGAGCC
CCCGCGGTTGCAGCCTCTAGCCCCTGGAAACGAGGAACGCCATGAACCGAAACGACCTGCGCCGCGTCGATCTGAACCTG
CTGATCGTGTTCGAGACCCTGATGCACGAACGCAGCGTGACCCGCGCCGCAGAGAAACTGTTCCTCGGCCAGCCGGCCAG
CCGGCCATCAGCGCCGCGCTGTCGCGCCTGCGCACGCTGTTCGACGACCCGCTGTTCGTCCGTACCGGACGCAGCATGGA
GCCCACCGCGCGAGCCCAGGAAATCTTCGCCCACCTGTCGCCGGCGCTGGATTCCATCTCCACCGCCATGAGTCGCGCCA
GCGAGTTCGATCCGGCGACCAGCACCGCGGTGTTCCGCATCGGCCTTTCCGACGACGTCGAGTTCGGCCTGTTGCCGCCC
CTGCTCCGCCGCCTGCGCGCGGAGGCGCCGGGGTTCGTCCTCGTCGTGCGCCGCGCCAACTATCTATTGATGCCGAACCT
GCTGGCCTCGGGGGAGATCTCGGTGGGCGTCAGCTACACCGACGAACTGCCGGCCAACGCCAAGCGCAAGACCGTGCGCC
GCAGCAAGCCGAAGATCCTCCGCGCCGACTCCGCGCCCGGCCAGCTGACCCTCGACGACTATTGCGCGCGACCGCACGCG
CTGGTGTCCTTCGCCGGCGACCTCAGCGGCTTCGTCGACGAGGAGCTGGAAAAATTCGGCCGCAAGCGCAAGGTGGTCCT
GGCGGTGCCGCAGTTCAACGGCCTCGGCACCCTCCTGGCCGGCACCGACATCATCGCCACCGTGCCCGACTACGCCGCCC
AGGCGCTGATCGCCGCCGGCGGCCTACGCGCCGAGGACCCACCGTTCGAGACCCGGGCCTTCGAACTGTCGATGGCTTGG
CGCGGCGCCCAGGACAACGATCCGGCCGAACGCTGGCTGCGCTCGCGGATCAGCATGTTCATCGGCGATCCGGACAGTCT
CTGA