Detailed information of regulator
Summary
| Regulator ID | REG0131  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Unknown |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00074  (Type i3) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome |
| Sequence | Protein sequence (214 a.a.); Nucleotide sequence (642 bp) |
| Description | TctR negatively regulates expression of the T6SS-2 gene cluster. |
| Reference |
External database links
| Locus tag (Gene) | BPSL3431 (TctR) |
| Coordinates (Strand) | 4073388..4074029 (-) |
| NCBI ID | 53721038 |
| RefSeq | NC_006350 |
| Uniprot ID | Q63PE5_BURPS |
| KEGG ID | bps:BPSL3431 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| T6SS-2 (hcp2) transcript | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MarR_2 | PF12802.9 | 2.2e-11 | 37..96 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-213 | MSDGVYGNQAAGRVTHSLLRLSTAMRSQAWDWAEGAGLTPTQGEILVLLLQRKGPMRLGEIARETQLTAATTSDAVSTLEHKGLVEKRRALDDGRALAVRLSARGRTAAKKALQWPDFLSKAVGTLGGDEQGVLYRALLKTLRELQVNGDIPPHRMCVTCKHFQPGKAARKPTYRCSLLDLTMTDSDLRLDCAVHEEADVLTQKKTWKLFAQA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9834 | - | - |
Protein sequence of regulator: 214 a.a. .
>REG0131 NC_006350:c4074029-4073388 [Burkholderia pseudomallei K96243]
MSDGVYGNQAAGRVTHSLLRLSTAMRSQAWDWAEGAGLTPTQGEILVLLLQRKGPMRLGEIARETQLTAATTSDAVSTLE
HKGLVEKRRALDDGRALAVRLSARGRTAAKKALQWPDFLSKAVGTLGGDEQGVLYRALLKTLRELQVNGDIPPHRMCVTC
KHFQPGKAARKPTYRCSLLDLTMTDSDLRLDCAVHEEADVLTQKKTWKLFAQA*
MSDGVYGNQAAGRVTHSLLRLSTAMRSQAWDWAEGAGLTPTQGEILVLLLQRKGPMRLGEIARETQLTAATTSDAVSTLE
HKGLVEKRRALDDGRALAVRLSARGRTAAKKALQWPDFLSKAVGTLGGDEQGVLYRALLKTLRELQVNGDIPPHRMCVTC
KHFQPGKAARKPTYRCSLLDLTMTDSDLRLDCAVHEEADVLTQKKTWKLFAQA*
Nucleotide sequence of regulator: 642 bp .
>REG0131 NC_006350:c4074029-4073388 [Burkholderia pseudomallei K96243]
ATGAGCGATGGTGTTTACGGGAACCAGGCTGCGGGGCGAGTGACCCACAGCCTGTTGCGGTTGAGCACGGCTATGCGGAG
CCAGGCATGGGATTGGGCGGAAGGCGCGGGGTTGACGCCGACGCAGGGTGAAATCCTCGTGCTGCTGCTCCAGCGTAAAG
GGCCGATGCGTCTGGGCGAGATTGCGCGCGAAACGCAGTTGACCGCCGCAACGACGAGTGATGCGGTGAGCACGCTCGAG
CACAAGGGGCTGGTCGAAAAGCGGCGCGCGCTCGACGACGGCCGCGCACTCGCGGTGCGCCTGAGCGCGCGCGGCCGCAC
GGCGGCGAAGAAGGCGCTGCAGTGGCCCGACTTCCTGTCGAAGGCGGTCGGCACGCTCGGCGGCGACGAGCAGGGCGTGC
TGTACCGCGCGCTGCTGAAGACGCTGCGTGAGCTGCAGGTCAACGGCGACATTCCGCCGCACCGGATGTGCGTGACGTGC
AAACATTTCCAGCCGGGCAAGGCGGCGCGCAAGCCGACGTACCGCTGTTCGCTGCTCGATCTGACGATGACGGACAGCGA
TCTGCGTCTCGATTGCGCGGTGCATGAGGAAGCGGACGTCCTGACGCAGAAGAAGACCTGGAAGCTCTTCGCGCAGGCGT
GA
ATGAGCGATGGTGTTTACGGGAACCAGGCTGCGGGGCGAGTGACCCACAGCCTGTTGCGGTTGAGCACGGCTATGCGGAG
CCAGGCATGGGATTGGGCGGAAGGCGCGGGGTTGACGCCGACGCAGGGTGAAATCCTCGTGCTGCTGCTCCAGCGTAAAG
GGCCGATGCGTCTGGGCGAGATTGCGCGCGAAACGCAGTTGACCGCCGCAACGACGAGTGATGCGGTGAGCACGCTCGAG
CACAAGGGGCTGGTCGAAAAGCGGCGCGCGCTCGACGACGGCCGCGCACTCGCGGTGCGCCTGAGCGCGCGCGGCCGCAC
GGCGGCGAAGAAGGCGCTGCAGTGGCCCGACTTCCTGTCGAAGGCGGTCGGCACGCTCGGCGGCGACGAGCAGGGCGTGC
TGTACCGCGCGCTGCTGAAGACGCTGCGTGAGCTGCAGGTCAACGGCGACATTCCGCCGCACCGGATGTGCGTGACGTGC
AAACATTTCCAGCCGGGCAAGGCGGCGCGCAAGCCGACGTACCGCTGTTCGCTGCTCGATCTGACGATGACGGACAGCGA
TCTGCGTCTCGATTGCGCGGTGCATGAGGAAGCGGACGTCCTGACGCAGAAGAAGACCTGGAAGCTCTTCGCGCAGGCGT
GA