SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0109
Regulator type	protein
Regulatory pathway (from literatures)	Unknown
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00043 (Type i1)
Strain	Vibrio parahaemolyticus RIMD 2210633
Replicon	chromosome
Sequence	Protein sequence (151 a.a.); Nucleotide sequence (453 bp)
Description	VP1407 is predicted to belong to the AsnC family of Transcription regulators and required for expression of Hcp1 under marine-like conditions.; TfoY‐mediated activation of T6SS1 was dependent on the presence of both vp1391 and vp1407.
Reference	[1] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791 [2] Ben-Yaakov R, Salomon D. The regulatory network of Vibrio parahaemolyticus type VI secretion system 1. Environ Microbiol. 2019 Jul;21(7):2248-2260. doi: 10.1111/1462-2920.14594. Epub 2019 Apr 2. PMID: 30882997

External database links

Locus tag (Gene)	vp1407
Coordinates (Strand)	1511565..1512017 (-)
NCBI ID	28898181
RefSeq	NC_004603
Uniprot ID	Q87PU4_VIBPA
KEGG ID	vpa:VP1407
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS1 (VgrG1) expression and secretion	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
HTH_AsnC-type	PF13404.8	8.7e-14	10..51
AsnC_trans_reg	PF01037.23	1.7e-12	77..145

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-150	MISNMTLQGLDKLDRKILSSLLTNGRESIANLSRNIGLSRTAVAERINRLEKTGIIKGYTAQIRIESDGRKAASYLLISCEKGKKNEVTNALKEIPEVRLTSVVGGSFDIIALIEAPDLQSIHHLCNEIESFNGIQKLDTTVVLHQPISR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9552	-	-

Protein sequence of regulator: 151 a.a. .

                                    >REG0109 NC_004603:c1512017-1511565 [Vibrio parahaemolyticus RIMD 2210633]
MISNMTLQGLDKLDRKILSSLLTNGRESIANLSRNIGLSRTAVAERINRLEKTGIIKGYTAQIRIESDGRKAASYLLISC
EKGKKNEVTNALKEIPEVRLTSVVGGSFDIIALIEAPDLQSIHHLCNEIESFNGIQKLDTTVVLHQPISR*

                                 

Nucleotide sequence of regulator: 453 bp .

                                    >REG0109 NC_004603:c1512017-1511565 [Vibrio parahaemolyticus RIMD 2210633]
ATGATTTCTAATATGACTTTACAAGGCTTAGATAAGTTAGATCGCAAAATACTTTCCAGCCTGTTAACAAACGGGCGTGA
GTCTATTGCAAATCTCTCTAGAAATATCGGTTTGTCCCGTACTGCTGTCGCAGAAAGGATCAATAGGTTGGAAAAAACCG
GTATTATAAAAGGCTATACCGCACAAATTCGGATTGAAAGTGACGGGAGAAAAGCTGCTAGCTATTTACTTATCTCATGT
GAAAAAGGAAAGAAAAACGAAGTAACTAACGCCCTAAAGGAAATTCCTGAAGTACGTCTTACGAGCGTAGTTGGCGGCAG
CTTCGATATTATTGCTTTGATTGAAGCACCAGACCTTCAGTCTATTCACCACCTTTGCAACGAAATTGAGTCTTTTAATG
GGATACAAAAACTAGACACTACGGTTGTACTTCATCAGCCGATCAGTCGATAG

                                