SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0055
Regulator type	protein
Regulatory pathway (from literatures)	Unknown
KEGG pathway (determined by BlastKOALA)	beta-Lactam resistance (map01501)
Related T6SS	T6SS00003 (Type i3)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (297 a.a.); Nucleotide sequence (891 bp)
Description	the proteome analysis shows negative regulation of proteins of the T6SS Tse3 (PA3484), TssJ1 (PA0080) and TssG1(PA0089). And upon β-lactam exposure, AmpR negatively regulates TssE1 (PA0087) and Tse2 (PA2702).
Reference	[1] Kumari H, Murugapiran SK, Balasubramanian D, et al. LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames. J Proteomics. 2014 Jan 16;96:328-342. doi: 10.1016/j.jprot.2013.11.018. Epub 2013 Nov 28. PMID: 24291602

External database links

Locus tag (Gene)	PA4109 (AmpR)
Coordinates (Strand)	4592990..4593880 (-)
NCBI ID	15599304
RefSeq	NC_002516
Uniprot ID	AMPR_PSEAE
KEGG ID	pae:PA4109
KEGG pathway (determined by BlastKOALA)	map01501
PDB ID	5MMH

Target

Target	Regulation (↓/↑)	Target sequence
T6SS transcripts	↑	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
LysR_substrate	PF03466.22	9.2e-34	91..289
HTH_1	PF00126.29	4e-20	8..67

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-296	MVRPHLPLNALRAFEASARHLSFTRAAIELCVTQAAVSHQVKSLEERLGVALFKRLPRGLMLTHEGESLLPVLCDSFDRIAGLLERFEGGHYRDVLTVGAVGTFTVGWLLPRLEDFQARHPFIDLRLSTHNNRVDIAAEGLDYAIRFGGGAWHGTEALALFEAPLTVLCCPEVAAQLHSPADLLQHTLLRSYRADEWPLWFQAAGLPAHAPLTRSIVFDTSLAMLEAARQGVGVALAPAAMFARQLASESIRRPFATEVSTGSYWLTRLQSRGETSAMLAFRGWLLEMAAVEARGR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9638	-	-

Protein sequence of regulator: 297 a.a. .

                                    >REG0055 NC_002516:c4593880-4592990 [Pseudomonas aeruginosa PAO1]
MVRPHLPLNALRAFEASARHLSFTRAAIELCVTQAAVSHQVKSLEERLGVALFKRLPRGLMLTHEGESLLPVLCDSFDRI
AGLLERFEGGHYRDVLTVGAVGTFTVGWLLPRLEDFQARHPFIDLRLSTHNNRVDIAAEGLDYAIRFGGGAWHGTEALAL
FEAPLTVLCCPEVAAQLHSPADLLQHTLLRSYRADEWPLWFQAAGLPAHAPLTRSIVFDTSLAMLEAARQGVGVALAPAA
MFARQLASESIRRPFATEVSTGSYWLTRLQSRGETSAMLAFRGWLLEMAAVEARGR*

                                 

Nucleotide sequence of regulator: 891 bp .

                                    >REG0055 NC_002516:c4593880-4592990 [Pseudomonas aeruginosa PAO1]
TTGGTTCGACCCCATTTGCCGCTGAACGCCCTGCGCGCCTTCGAAGCTTCGGCCCGGCACCTGAGCTTCACCCGCGCGGC
CATCGAGCTGTGCGTGACCCAGGCGGCGGTCAGCCACCAGGTGAAGAGCCTCGAGGAGCGTCTCGGCGTGGCCCTGTTCA
AGCGTCTGCCGCGCGGCCTCATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTGCTGTGTGACTCCTTCGACCGCATC
GCCGGCCTGCTGGAACGTTTCGAGGGTGGCCACTACCGGGACGTGCTCACCGTCGGCGCGGTCGGAACCTTCACGGTCGG
TTGGCTGCTGCCGCGGCTGGAGGACTTCCAGGCGCGCCATCCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCG
TCGACATCGCCGCCGAGGGGCTCGACTACGCGATCCGCTTCGGCGGCGGCGCCTGGCACGGCACCGAGGCGCTGGCGTTG
TTCGAGGCGCCGCTGACGGTGCTCTGCTGCCCGGAGGTCGCCGCCCAGTTGCACAGTCCCGCCGACCTGCTGCAGCACAC
CCTGCTGCGCTCCTACCGCGCCGACGAGTGGCCGCTGTGGTTCCAGGCGGCCGGACTGCCGGCGCACGCGCCACTGACCC
GGAGCATCGTCTTCGACACCTCGCTGGCCATGCTCGAGGCGGCCCGCCAGGGTGTCGGCGTGGCCCTGGCGCCGGCGGCG
ATGTTTGCCCGGCAACTGGCCAGCGAGAGCATCCGGCGTCCGTTCGCCACCGAAGTGAGTACCGGCAGCTACTGGCTGAC
GCGCTTGCAGTCGCGGGGGGAGACCAGCGCGATGCTGGCGTTCCGGGGGTGGTTGCTGGAGATGGCTGCCGTTGAGGCGC
GGGGGAGATAA

                                