Detailed information of regulator
Summary
| Regulator ID | REG0371  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Unknown |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS17506 (Type i1) |
| Strain | Aliivibrio fischeri strain ES401 |
| Replicon | chromosome |
| Sequence | Protein sequence (150 a.a.); Nucleotide sequence (450 bp) |
| Description | T6SS expression requires conserved structural proteins, in addition to the essential regulator TasR. |
| Reference |
External database links
| Locus tag (Gene) | VFES401_RS15770 (tasR) |
| Coordinates (Strand) | 373862..374311 (+) |
| NCBI ID | WP_012535053.1 |
| RefSeq | NZ_SRJG01000005 |
| Uniprot ID | B5EUJ9_ALIFM, A0A1B9P6J0_ALIFS |
| KEGG ID | vfm:VFMJ11_A0818 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| hcp promotor | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| HTH_AsnC-type | PF13404.8 | 1.2e-13 | 9..50 |
| AsnC_trans_reg | PF01037.23 | 6e-09 | 76..144 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-149 | MSNMNLQGLDKLDRRIISNLLSNGRESIANLSRNVGLSRTAVAERINRLEKTGIIKGYTAQIRMGNKDENASSYLLISCNKGQKDSVSDGLKEIPEVKMTSIVGGTYDIICLIEAPDLHRIHYLCSEIESLSGIKEVKNTIVLHQPVCR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9544 | - | - |
Protein sequence of regulator: 150 a.a. .
>REG0371 NZ_SRJG01000005:373862-374311 [Aliivibrio fischeri]
MSNMNLQGLDKLDRRIISNLLSNGRESIANLSRNVGLSRTAVAERINRLEKTGIIKGYTAQIRMGNKDENASSYLLISCN
KGQKDSVSDGLKEIPEVKMTSIVGGTYDIICLIEAPDLHRIHYLCSEIESLSGIKEVKNTIVLHQPVCR*
MSNMNLQGLDKLDRRIISNLLSNGRESIANLSRNVGLSRTAVAERINRLEKTGIIKGYTAQIRMGNKDENASSYLLISCN
KGQKDSVSDGLKEIPEVKMTSIVGGTYDIICLIEAPDLHRIHYLCSEIESLSGIKEVKNTIVLHQPVCR*
Nucleotide sequence of regulator: 450 bp .
>REG0371 NZ_SRJG01000005:373862-374311 [Aliivibrio fischeri]
ATGTCAAACATGAATCTACAAGGCTTGGATAAATTAGATAGAAGAATTATCTCTAATCTATTATCAAACGGTAGAGAGTC
TATTGCGAATCTTTCGCGTAACGTAGGTTTATCACGAACAGCGGTTGCTGAACGTATAAACCGACTTGAGAAAACGGGGA
TCATTAAAGGATATACAGCGCAAATCCGCATGGGAAATAAAGATGAAAATGCATCATCTTATTTACTTATCTCATGTAAT
AAAGGACAAAAAGACAGTGTTTCTGATGGATTAAAAGAAATTCCAGAAGTGAAAATGACGAGTATTGTTGGAGGAACTTA
CGATATTATCTGTTTAATTGAAGCGCCCGATTTACACCGAATTCATTACTTATGTAGCGAAATCGAATCGTTATCGGGCA
TAAAAGAAGTGAAAAATACCATCGTGTTGCACCAGCCAGTGTGTCGATAA
ATGTCAAACATGAATCTACAAGGCTTGGATAAATTAGATAGAAGAATTATCTCTAATCTATTATCAAACGGTAGAGAGTC
TATTGCGAATCTTTCGCGTAACGTAGGTTTATCACGAACAGCGGTTGCTGAACGTATAAACCGACTTGAGAAAACGGGGA
TCATTAAAGGATATACAGCGCAAATCCGCATGGGAAATAAAGATGAAAATGCATCATCTTATTTACTTATCTCATGTAAT
AAAGGACAAAAAGACAGTGTTTCTGATGGATTAAAAGAAATTCCAGAAGTGAAAATGACGAGTATTGTTGGAGGAACTTA
CGATATTATCTGTTTAATTGAAGCGCCCGATTTACACCGAATTCATTACTTATGTAGCGAAATCGAATCGTTATCGGGCA
TAAAAGAAGTGAAAAATACCATCGTGTTGCACCAGCCAGTGTGTCGATAA