SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0132
Regulator type	protein
Regulatory pathway (from literatures)	Unknown
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00076 (Type i3); T6SS00077 (Type i4b)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Description	BpsΔppiB strain increases in multiple components of the T6SS-3s.; TctR positively regulates expression of the T6SS-6 gene cluster.
Reference	[1] Bzdyl NM, Scott NE, Norville IH, et al. Peptidyl-Prolyl Isomerase ppiB Is Essential for Proteome Homeostasis and Virulence in Burkholderia pseudomallei. Infect Immun. 2019 Sep 19;87(10). pii: e00528-19. doi: 10.1128/IAI.00528-19. Print 2019 Oct. PMID: 31331957 [2] Losada L, Shea AA, DeShazer D. A MarR family transcriptional regulator and subinhibitory antibiotics regulate type VI secretion gene clusters in Burkholderia pseudomallei. Microbiology (Reading). 2018 Sep;164(9):1196-1211. doi: 10.1099/mic.0.000697. Epub 2018 Jul 27. PMID: 30052173

External database links

Locus tag (Gene)	BPSL2246 (PpiB)
Coordinates (Strand)	2708622..2709113 (+)
NCBI ID	53719855
RefSeq	NC_006350
Uniprot ID	Q63SS5_BURPS
KEGG ID	bps:BPSL2246
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

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Target	Regulation (↓/↑)	Target sequence
T6SS-3 (BPSS2099/TssCIII and BPSS2098/HcpIII) expression	↑	-
T6SS-6 (hcp6) transcript	↓	-

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Pfam domain hit(s) of regulator

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Domain	Pfam ID	E-value	Aligned region
Pro_isomerase	PF00160.23	1.8e-47	1..160

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Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction	Region	Sequence
Outside	1-163	MVELHTNHGVIKLELDEAKAPKTVENFLNYVKKGHYDGTIFHRVINGFMIQGGGFEPGLKQKPTDAPIANEANNGLKNDTYTIAMARTNDPHSATAQFFINVNDNEFLNHSSPTPQGWGYAVFGKVVEGQDIVDKIKAVKTGSKGFHQDVPNDDVVIEKAVVV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

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Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9883	-	-

Protein sequence of regulator: 164 a.a. .

                                    >REG0132 NC_006350:2708622-2709113 [Burkholderia pseudomallei K96243]
MVELHTNHGVIKLELDEAKAPKTVENFLNYVKKGHYDGTIFHRVINGFMIQGGGFEPGLKQKPTDAPIANEANNGLKNDT
YTIAMARTNDPHSATAQFFINVNDNEFLNHSSPTPQGWGYAVFGKVVEGQDIVDKIKAVKTGSKGFHQDVPNDDVVIEKA
VVV*

                                 

Nucleotide sequence of regulator: 492 bp .

                                    >REG0132 NC_006350:2708622-2709113 [Burkholderia pseudomallei K96243]
ATGGTCGAACTGCATACGAACCACGGCGTCATCAAGCTCGAACTCGACGAAGCGAAGGCGCCGAAGACGGTCGAGAACTT
CCTGAACTACGTGAAGAAGGGCCACTACGACGGCACGATCTTCCATCGCGTGATCAACGGTTTCATGATCCAGGGCGGCG
GCTTCGAGCCGGGCCTGAAGCAAAAGCCGACCGACGCGCCGATCGCGAACGAAGCGAACAACGGCCTGAAGAACGATACG
TATACGATCGCGATGGCGCGCACGAACGATCCGCACTCGGCGACCGCGCAGTTCTTCATCAACGTGAACGACAACGAATT
CCTGAACCACTCGTCGCCGACCCCGCAAGGCTGGGGCTACGCGGTGTTCGGCAAGGTCGTCGAAGGCCAGGACATCGTCG
ACAAGATCAAGGCCGTCAAGACGGGCAGCAAGGGCTTCCATCAGGACGTGCCGAACGACGACGTCGTGATCGAGAAGGCC
GTCGTGGTCTGA

                                