Detailed information of accessory protein


Summary

Accessory protein ID ACP00005 experimental
Type adaptor
Related T6SS (Type) T6SS00004 (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Related structure protein PA0262 (VgrG2b)
Related effector PA0260 (Tle3)
Description Tla3 is appropriate with a recruiting role of Tle3 to the H2-T6SS machinery through an interaction with the TTR domain of VgrG2b
Reference [1] Berni B, Soscia C, Djermoun S, et al. A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa. Front Microbiol. 2019 Jun 5;10:1218. doi: 10.3389/fmicb.2019.01218. eCollection 2019. PMID: 31231326
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) PA0259 (tla3)
Coordinates (Strand) 289562..291004 (-)
NCBI ID 15595456
RefSeq NC_002516
Uniprot ID Q9I6N0_PSEAE
KEGG ID pae:PA0259
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF2875 PF11394.10 3.2e-30 252..417
DUF2875 PF11394.10 1.9e-11 17..240
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-480 MSADAYHPPTTSPRLETLDVLSIGMSLDVFRQGQVWKALQEQNAAQAEALHVGSILPMDPKKYPTSADDKDMAYEKRQADALELGLKNFLEKWPIPTVTVVRGWDPSTPNLRFTPEETRESLSVKVNDLRVPAGLHWHRIANLQDGIICNDTPEGVLEALFSLFERNPDLPAVLVYANEGISMAGALSSRDVTLKSLGAVSGPRIPGTLTDAMVALIVGRPERVDWLRQFAPYTKVNENRIDPEFRGWGWRKPPVEFRPTPFIPQPWTERALEQWDALPVLARLHRPVSVPLTHPDTGERLKREALTAQLAAAWKTASAGLRPAPARLFYDGGLNATPLAELTPALGAAQSSLDLLDSRESYDLTQRLGDTGAASPFVGITLATMASYLNGDSSMVMPLRRKDQATLIGISSPTPGKKPVHDPFGVDLLPQTASGDGPPPSADPVAPASRLTTRLPPGEDYALEEFLSSLKPSTDWQDDL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9922 - -


  Protein sequence: 481 a.a.    

>ACP00005 NC_002516:c291004-289562 [Pseudomonas aeruginosa PAO1]
MSADAYHPPTTSPRLETLDVLSIGMSLDVFRQGQVWKALQEQNAAQAEALHVGSILPMDPKKYPTSADDKDMAYEKRQAD
ALELGLKNFLEKWPIPTVTVVRGWDPSTPNLRFTPEETRESLSVKVNDLRVPAGLHWHRIANLQDGIICNDTPEGVLEAL
FSLFERNPDLPAVLVYANEGISMAGALSSRDVTLKSLGAVSGPRIPGTLTDAMVALIVGRPERVDWLRQFAPYTKVNENR
IDPEFRGWGWRKPPVEFRPTPFIPQPWTERALEQWDALPVLARLHRPVSVPLTHPDTGERLKREALTAQLAAAWKTASAG
LRPAPARLFYDGGLNATPLAELTPALGAAQSSLDLLDSRESYDLTQRLGDTGAASPFVGITLATMASYLNGDSSMVMPLR
RKDQATLIGISSPTPGKKPVHDPFGVDLLPQTASGDGPPPSADPVAPASRLTTRLPPGEDYALEEFLSSLKPSTDWQDDL
*

  Nucleotide sequence: 1443 bp    

>ACP00005 NC_002516:c291004-289562 [Pseudomonas aeruginosa PAO1]
ATGTCCGCTGATGCTTATCACCCACCGACAACTTCGCCGCGCCTGGAAACCCTGGACGTGCTCAGCATAGGCATGAGCCT
GGATGTGTTTCGCCAGGGGCAAGTGTGGAAGGCCTTGCAGGAGCAGAATGCCGCACAGGCCGAGGCGTTGCATGTCGGCA
GCATCCTGCCGATGGACCCGAAGAAGTATCCGACGTCTGCGGATGACAAGGATATGGCGTATGAAAAGCGCCAAGCGGAC
GCCTTGGAACTAGGGCTGAAAAACTTCCTGGAGAAATGGCCGATACCGACGGTGACGGTGGTGCGTGGTTGGGATCCGAG
CACTCCTAACCTACGCTTCACTCCGGAGGAGACGCGGGAAAGCCTGTCAGTAAAAGTCAATGATCTCCGTGTTCCGGCTG
GACTGCATTGGCATCGGATTGCCAATTTGCAGGATGGCATTATCTGTAACGATACGCCTGAAGGAGTACTGGAGGCGTTG
TTCAGTTTATTCGAACGCAACCCTGACTTGCCAGCAGTGTTGGTGTATGCGAATGAAGGCATCAGTATGGCGGGGGCGCT
TTCCAGTAGAGATGTAACATTGAAGTCACTAGGCGCTGTGTCAGGCCCTCGGATACCTGGAACGCTTACGGATGCAATGG
TCGCCCTGATCGTCGGCCGCCCCGAGCGGGTCGACTGGCTACGCCAGTTCGCCCCCTACACCAAGGTCAACGAAAACCGG
ATCGACCCCGAGTTCCGTGGCTGGGGCTGGCGCAAGCCGCCGGTGGAGTTCCGCCCCACGCCGTTCATTCCCCAGCCCTG
GACCGAGCGGGCTCTGGAGCAGTGGGATGCGTTGCCGGTGCTGGCCAGGCTGCATCGGCCGGTGAGTGTGCCGCTGACCC
ACCCGGATACCGGCGAACGGCTCAAGCGCGAAGCTCTCACCGCACAACTGGCCGCTGCCTGGAAGACGGCCAGTGCCGGG
CTCAGGCCGGCGCCCGCCCGGCTGTTCTACGACGGCGGTTTGAACGCCACGCCGCTGGCCGAACTCACCCCGGCGCTGGG
CGCCGCGCAGAGTTCGCTGGACTTGCTGGACTCGCGGGAAAGCTACGACCTGACCCAGCGCCTGGGCGACACCGGCGCGG
CCTCGCCCTTCGTTGGCATCACCCTGGCGACCATGGCCAGCTACCTCAACGGCGACAGCAGCATGGTGATGCCGTTGCGG
CGCAAGGACCAGGCGACCCTGATCGGCATCAGCTCGCCGACGCCGGGGAAGAAGCCCGTTCACGACCCGTTCGGCGTCGA
CCTGCTGCCGCAGACCGCCAGCGGCGACGGTCCGCCGCCATCGGCCGACCCGGTGGCGCCCGCCAGTCGGCTCACGACCC
GGTTGCCGCCCGGCGAAGACTATGCCCTGGAAGAGTTCCTGAGCAGCCTCAAACCCAGCACCGACTGGCAGGACGATTTA
TAG