SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01450
Effector type	Tle
Cognate immunity protein	IMU00315
Related T6SS (Type)	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (717 a.a.); Nucleotide sequence (2151 bp)
Description	-
Reference	[1] Berni B, Soscia C, Djermoun S, et al. A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa. Front Microbiol. 2019 Jun 5;10:1218. doi: 10.3389/fmicb.2019.01218. eCollection 2019. PMID: 31231326

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA0260
Coordinates (Strand)	291154..293304 (-)
NCBI ID	15595457
RefSeq	NC_002516
Uniprot ID	Q9I6M9_PSEAE
KEGG ID	pae:PA0260
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DUF3274	PF11678.10	5.6e-109	386..685

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-716	MNDRVRSRTIVSAQSITLPKGGDVHLVPPPPKPCVTIVVHGVNDLAGCYERIERGLCQGLNERLDMPPTLPGGQANPGYLTPAGYSLPADDEGKAENPDVVYYRRKFASGAGGAAVRSVVVPFYWGFREEEQYINKTAAHGEWLDRNGNRLDKSGTKEGGQFVNATTNLPDMWGQGFNGKLFGFISLDWFGGTMTHPLFSAAGRKYMVLAAMRLAMLIKIIRKRYPDDTINVVGHSQGTLLTLLAHAFLKDDGVAPADGVIMLNSPYGLFEPLNEKLQGWSSQQTREARLATLKGILEFICGRRHPVPALSSVALRNCQGYGAIGGPGWVGGQGCQTTIDGERLSFDERDNRGSVYLYFTPQDQTVGLANVQGIGWRGIAEQVKGLPGRTGLPQGFHQRIFTVRKRNGEKEKIGGHAPPHVYPLLLAGEKTWEDTGLGGKDRFGRANFDQGDSVLLTAPRLPLPTEARFDFDGAVTAPGENSASGVYQVRDTLDPIDAAIGVSNGGWKEKDSGHAVAQQVDAALAYRYGRDARSVERALNEGKELAQQTHVFSARELGTGMVLVTRAETPYEARLRLQTAEGHLEPLSFHSAIPNNPEHNRRVLAYDLAIGAGDSVDDVVFYQYLCRVADWRLDWKASDKGIFSQGDASVDLPDEEVRALYRAEESKNSQLIDATVAYRKSGEFPVVVGNRLPSLVGTQTILDRYHEQAVRFGGTI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8490	-	-

Protein sequence: 717 a.a. .

                                    >EFF01450 NC_002516:c293304-291154 Type VI Lipase Effector (Tle) [Pseudomonas aeruginosa PAO1]
MNDRVRSRTIVSAQSITLPKGGDVHLVPPPPKPCVTIVVHGVNDLAGCYERIERGLCQGLNERLDMPPTLPGGQANPGYL
TPAGYSLPADDEGKAENPDVVYYRRKFASGAGGAAVRSVVVPFYWGFREEEQYINKTAAHGEWLDRNGNRLDKSGTKEGG
QFVNATTNLPDMWGQGFNGKLFGFISLDWFGGTMTHPLFSAAGRKYMVLAAMRLAMLIKIIRKRYPDDTINVVGHSQGTL
LTLLAHAFLKDDGVAPADGVIMLNSPYGLFEPLNEKLQGWSSQQTREARLATLKGILEFICGRRHPVPALSSVALRNCQG
YGAIGGPGWVGGQGCQTTIDGERLSFDERDNRGSVYLYFTPQDQTVGLANVQGIGWRGIAEQVKGLPGRTGLPQGFHQRI
FTVRKRNGEKEKIGGHAPPHVYPLLLAGEKTWEDTGLGGKDRFGRANFDQGDSVLLTAPRLPLPTEARFDFDGAVTAPGE
NSASGVYQVRDTLDPIDAAIGVSNGGWKEKDSGHAVAQQVDAALAYRYGRDARSVERALNEGKELAQQTHVFSARELGTG
MVLVTRAETPYEARLRLQTAEGHLEPLSFHSAIPNNPEHNRRVLAYDLAIGAGDSVDDVVFYQYLCRVADWRLDWKASDK
GIFSQGDASVDLPDEEVRALYRAEESKNSQLIDATVAYRKSGEFPVVVGNRLPSLVGTQTILDRYHEQAVRFGGTI*

                                

Nucleotide sequence: 2151 bp .

                                    >EFF01450 NC_002516:c293304-291154 Type VI Lipase Effector (Tle) [Pseudomonas aeruginosa PAO1]
ATGAACGATAGGGTTCGGTCGAGGACGATCGTCTCTGCCCAGTCCATCACCTTGCCCAAGGGCGGCGACGTGCACCTGGT
GCCGCCTCCGCCCAAGCCTTGCGTGACCATCGTGGTGCATGGCGTCAACGATCTCGCGGGTTGCTACGAACGGATCGAGC
GAGGGCTCTGCCAGGGGCTCAATGAACGCCTGGACATGCCGCCGACCTTGCCCGGCGGGCAGGCCAATCCCGGCTACCTG
ACGCCGGCGGGCTACAGCCTGCCGGCGGACGACGAAGGCAAGGCAGAGAACCCCGACGTCGTCTACTACCGGCGCAAGTT
CGCCAGTGGCGCCGGCGGGGCCGCCGTACGCAGCGTAGTCGTACCTTTCTACTGGGGCTTCCGCGAGGAAGAGCAATACA
TCAACAAGACCGCGGCCCACGGCGAATGGCTGGACCGCAACGGCAACCGGCTGGACAAGTCCGGCACCAAGGAAGGCGGG
CAGTTCGTCAATGCCACCACCAACCTGCCGGACATGTGGGGCCAGGGTTTCAACGGCAAGCTGTTCGGTTTCATCTCGCT
GGACTGGTTCGGCGGCACCATGACCCATCCGCTGTTTTCGGCGGCAGGGCGCAAGTACATGGTCCTTGCGGCCATGCGCC
TGGCCATGTTGATCAAGATCATCCGCAAGCGTTACCCGGACGACACCATCAATGTCGTCGGGCACAGCCAGGGCACCCTG
TTGACCCTGCTGGCACATGCCTTCCTCAAGGATGACGGCGTGGCGCCGGCGGACGGGGTGATCATGCTCAACTCACCCTA
CGGCCTGTTCGAACCCCTCAACGAGAAGCTCCAGGGCTGGAGCAGCCAGCAGACCAGAGAGGCGCGGCTGGCCACGCTCA
AAGGTATCCTCGAGTTCATCTGCGGCCGACGCCATCCGGTCCCCGCGCTGAGCAGCGTCGCGTTGCGCAACTGCCAAGGC
TACGGCGCGATAGGCGGCCCCGGCTGGGTGGGTGGCCAGGGCTGCCAGACGACCATCGACGGGGAACGGCTCAGCTTCGA
TGAGCGCGACAATCGCGGCAGTGTCTACCTGTATTTCACGCCCCAGGACCAGACGGTGGGACTTGCCAACGTCCAGGGTA
TCGGCTGGCGCGGCATTGCTGAGCAGGTCAAGGGGCTCCCGGGAAGGACCGGGCTGCCGCAGGGTTTTCACCAGCGCATC
TTCACCGTACGCAAGCGCAATGGCGAGAAGGAAAAGATCGGCGGGCACGCCCCTCCCCATGTCTATCCTCTTCTGCTGGC
CGGCGAGAAGACCTGGGAAGACACCGGGCTGGGCGGCAAGGACAGATTCGGCCGGGCGAACTTCGACCAGGGCGACAGCG
TGCTGCTGACTGCGCCGCGTCTGCCGTTGCCGACGGAAGCGCGATTCGATTTCGACGGAGCCGTCACGGCGCCGGGAGAG
AACTCCGCCAGCGGCGTCTATCAGGTGCGCGATACCCTCGATCCGATCGACGCCGCCATCGGCGTAAGCAACGGCGGCTG
GAAGGAAAAGGACAGCGGGCACGCCGTCGCGCAACAGGTGGACGCCGCGCTGGCGTACCGCTACGGGCGCGACGCGCGCA
GCGTGGAGAGGGCGTTGAACGAAGGCAAGGAATTGGCCCAGCAGACCCATGTGTTTTCCGCTCGTGAACTGGGCACCGGC
ATGGTTCTGGTCACCCGCGCGGAAACTCCCTACGAGGCCCGGCTGCGCCTGCAGACGGCGGAGGGCCATCTGGAGCCCTT
GTCGTTCCACTCGGCCATACCCAACAACCCGGAGCATAACCGGCGGGTACTGGCCTATGACCTGGCCATCGGGGCCGGGG
ACAGCGTGGATGATGTGGTTTTCTATCAGTATCTGTGTCGGGTGGCGGATTGGCGGTTGGATTGGAAGGCTTCGGACAAA
GGAATATTTAGTCAAGGTGATGCGTCTGTTGATCTGCCGGATGAAGAGGTGCGTGCTTTGTACCGTGCGGAAGAGTCTAA
AAACAGCCAGTTAATAGACGCCACCGTAGCTTACCGCAAGAGTGGCGAGTTCCCTGTGGTAGTAGGAAATAGACTGCCTT
CCCTAGTCGGGACACAAACCATCCTAGATCGTTACCACGAGCAGGCGGTGCGCTTTGGGGGGACAATCTAA

                                