Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00315  |
| Cognate effector | EFF01450; EFF01496  ; EFF01450; EFF01496 |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (166 a.a.); Nucleotide sequence (498 bp) |
| Description | PA0261 neutralizes VgrG2bC-ter through complex formation, as has been shown for other biochemically characterized T6SS toxin- immunity pairs and directly interacts with Tle3 then neutralizes Tle3 toxicity. |
| Reference | [1] Berni B, Soscia C, Djermoun S, et al. A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa. Front Microbiol. 2019 Jun 5;10:1218. doi: 10.3389/fmicb.2019.01218. eCollection 2019. PMID: 31231326 [2] Wood TE, Howard SA, Förster A, et al. The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology. Cell Rep. 2019 Oct 1;29(1):187-201.e7. doi: 10.1016/j.celrep.2019.08.094. PMID: 31577948 |
Experimental investigation has been performed. Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA0261 (Tli3) |
| Coordinates (Strand) | 293301..293798 (-) |
| NCBI ID | 15595458 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I6M8_PSEAE |
| KEGG ID | pae:PA0261 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-165 | MKCKTLLIACLFGLGSAQALAVSKLPPQIPVHAGSGRVTLDLRPLLAETNDVEITRVSVCRRVGSHCQETLWRIELPPGWRAGEIEVFGDYPGSSVLLRRPERLQPDGSYNAFIHFNERSRRHRQTVSSIAVEFCLAGEPGNWMLLDEATCLARRNAEERQGEKP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9933 | pos: 21-22. ALA-VS. | MKCKTLLIACLFGLGSAQALA |
Protein sequence: 166 a.a. .
>IMU00315 NC_002516:c293798-293301 [Pseudomonas aeruginosa PAO1]
MKCKTLLIACLFGLGSAQALAVSKLPPQIPVHAGSGRVTLDLRPLLAETNDVEITRVSVCRRVGSHCQETLWRIELPPGW
RAGEIEVFGDYPGSSVLLRRPERLQPDGSYNAFIHFNERSRRHRQTVSSIAVEFCLAGEPGNWMLLDEATCLARRNAEER
QGEKP*
MKCKTLLIACLFGLGSAQALAVSKLPPQIPVHAGSGRVTLDLRPLLAETNDVEITRVSVCRRVGSHCQETLWRIELPPGW
RAGEIEVFGDYPGSSVLLRRPERLQPDGSYNAFIHFNERSRRHRQTVSSIAVEFCLAGEPGNWMLLDEATCLARRNAEER
QGEKP*
Nucleotide sequence: 498 bp .
>IMU00315 NC_002516:c293798-293301 [Pseudomonas aeruginosa PAO1]
ATGAAATGCAAGACCTTGCTCATTGCCTGCCTGTTCGGCCTGGGAAGCGCCCAGGCCCTGGCCGTTTCCAAACTGCCACC
GCAGATCCCGGTGCACGCCGGCTCCGGGCGCGTGACACTGGATCTACGCCCCTTGCTGGCGGAAACCAACGACGTCGAGA
TCACCCGTGTTTCGGTATGTCGGCGGGTCGGCAGCCATTGCCAGGAAACGCTGTGGCGGATCGAGCTCCCGCCCGGGTGG
CGTGCCGGGGAGATCGAGGTCTTCGGCGACTATCCCGGCTCCAGCGTGCTCCTGCGCCGGCCGGAGCGCTTGCAGCCAGA
TGGGAGCTACAACGCATTCATTCATTTCAACGAGCGTAGCCGTCGGCACCGGCAAACGGTTTCCAGCATTGCCGTCGAAT
TCTGCCTGGCCGGCGAACCTGGCAACTGGATGCTGCTCGACGAAGCGACCTGCCTGGCACGACGCAACGCCGAGGAGCGG
CAAGGAGAGAAGCCATGA
ATGAAATGCAAGACCTTGCTCATTGCCTGCCTGTTCGGCCTGGGAAGCGCCCAGGCCCTGGCCGTTTCCAAACTGCCACC
GCAGATCCCGGTGCACGCCGGCTCCGGGCGCGTGACACTGGATCTACGCCCCTTGCTGGCGGAAACCAACGACGTCGAGA
TCACCCGTGTTTCGGTATGTCGGCGGGTCGGCAGCCATTGCCAGGAAACGCTGTGGCGGATCGAGCTCCCGCCCGGGTGG
CGTGCCGGGGAGATCGAGGTCTTCGGCGACTATCCCGGCTCCAGCGTGCTCCTGCGCCGGCCGGAGCGCTTGCAGCCAGA
TGGGAGCTACAACGCATTCATTCATTTCAACGAGCGTAGCCGTCGGCACCGGCAAACGGTTTCCAGCATTGCCGTCGAAT
TCTGCCTGGCCGGCGAACCTGGCAACTGGATGCTGCTCGACGAAGCGACCTGCCTGGCACGACGCAACGCCGAGGAGCGG
CAAGGAGAGAAGCCATGA