Detailed information of immunity protein


Summary

Immunity protein ID IMU00315 experimental
Cognate effector EFF01450; EFF01496 insolico; EFF01450; EFF01496 insolico
Related T6SS (Type) T6SS00004 experimental (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (166 a.a.); Nucleotide sequence (498 bp)
Description PA0261 neutralizes VgrG2bC-ter through complex formation, as has been shown for other biochemically characterized T6SS toxin- immunity pairs and directly interacts with Tle3 then neutralizes Tle3 toxicity.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) PA0261 (Tli3)
Coordinates (Strand) 293301..293798 (-)
NCBI ID 15595458
RefSeq NC_002516
Uniprot ID Q9I6M8_PSEAE
KEGG ID pae:PA0261
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-165 MKCKTLLIACLFGLGSAQALAVSKLPPQIPVHAGSGRVTLDLRPLLAETNDVEITRVSVCRRVGSHCQETLWRIELPPGWRAGEIEVFGDYPGSSVLLRRPERLQPDGSYNAFIHFNERSRRHRQTVSSIAVEFCLAGEPGNWMLLDEATCLARRNAEERQGEKP



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9933 pos: 21-22. ALA-VS. MKCKTLLIACLFGLGSAQALA



  Protein sequence: 166 a.a.    .

>IMU00315 NC_002516:c293798-293301 [Pseudomonas aeruginosa PAO1]
MKCKTLLIACLFGLGSAQALAVSKLPPQIPVHAGSGRVTLDLRPLLAETNDVEITRVSVCRRVGSHCQETLWRIELPPGW
RAGEIEVFGDYPGSSVLLRRPERLQPDGSYNAFIHFNERSRRHRQTVSSIAVEFCLAGEPGNWMLLDEATCLARRNAEER
QGEKP*

  Nucleotide sequence: 498 bp    .

>IMU00315 NC_002516:c293798-293301 [Pseudomonas aeruginosa PAO1]
ATGAAATGCAAGACCTTGCTCATTGCCTGCCTGTTCGGCCTGGGAAGCGCCCAGGCCCTGGCCGTTTCCAAACTGCCACC
GCAGATCCCGGTGCACGCCGGCTCCGGGCGCGTGACACTGGATCTACGCCCCTTGCTGGCGGAAACCAACGACGTCGAGA
TCACCCGTGTTTCGGTATGTCGGCGGGTCGGCAGCCATTGCCAGGAAACGCTGTGGCGGATCGAGCTCCCGCCCGGGTGG
CGTGCCGGGGAGATCGAGGTCTTCGGCGACTATCCCGGCTCCAGCGTGCTCCTGCGCCGGCCGGAGCGCTTGCAGCCAGA
TGGGAGCTACAACGCATTCATTCATTTCAACGAGCGTAGCCGTCGGCACCGGCAAACGGTTTCCAGCATTGCCGTCGAAT
TCTGCCTGGCCGGCGAACCTGGCAACTGGATGCTGCTCGACGAAGCGACCTGCCTGGCACGACGCAACGCCGAGGAGCGG
CAAGGAGAGAAGCCATGA