SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01889
Effector type	Tie
Cognate immunity protein	-
Related T6SS (Type)	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (252 a.a.); Nucleotide sequence (756 bp)
Description	H2-T6SS and the molybdate binding protein ModA are critical for bacterial virulence and T6SS-dependent molybdate acquisition in bacterial competition under anaerobic conditions.
Reference	[1] Wang T, Du X, Ji L, et al. Pseudomonas aeruginosa T6SS-mediated molybdate transport contributes to bacterial competition during anaerobiosis. Cell Rep. 2021 Apr 13;35(2):108957. doi: 10.1016/j.celrep.2021.108957. PMID: 33852869

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA1863 (ModA)
Coordinates (Strand)	2022411..2023166 (-)
NCBI ID	15597060
RefSeq	NC_002516
Uniprot ID	Q9I2N2_PSEAE
KEGG ID	pae:PA1863
PDB ID	-

Pfam domain hit(s)

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Domain	Pfam ID	E-value	Aligned region
SBP_bac_11	PF13531.8	3.4e-44	26..250

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Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction	Region	Sequence
Outside	1-251	MTTRLPQLLLALLASAVSLAASADEVQVAVAANFTAPIQAIAKEFEKDTGHRLVAAYGATGQFYTQIKNGAPFQVFLSADDSTPAKLEQEGEVVPGSRFTYAIGTLALWSPKAGYVDAEGEVLKSGSFRHLSIANPKTAPYGLAATQAMDKLGLAATLGPKLVEGQNISQAYQFVSSGNAELGFVALSQIYKDGKVATGSAWIVPTELHDPIRQDAVILNKGKDNAAAKALVDYLKGAKAAALIKSYGYEL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

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Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9919	pos: 23-24. ASA-DE.	MTTRLPQLLLALLASAVSLAASA

Protein sequence: 252 a.a. .

                                    >EFF01889 NC_002516:c2023166-2022411 Type VI Metal Ion acquisition Effector (Tie) [Pseudomonas aeruginosa PAO1]
MTTRLPQLLLALLASAVSLAASADEVQVAVAANFTAPIQAIAKEFEKDTGHRLVAAYGATGQFYTQIKNGAPFQVFLSAD
DSTPAKLEQEGEVVPGSRFTYAIGTLALWSPKAGYVDAEGEVLKSGSFRHLSIANPKTAPYGLAATQAMDKLGLAATLGP
KLVEGQNISQAYQFVSSGNAELGFVALSQIYKDGKVATGSAWIVPTELHDPIRQDAVILNKGKDNAAAKALVDYLKGAKA
AALIKSYGYEL*

                                

Nucleotide sequence: 756 bp .

                                    >EFF01889 NC_002516:c2023166-2022411 Type VI Metal Ion acquisition Effector (Tie) [Pseudomonas aeruginosa PAO1]
ATGACCACCCGCCTGCCCCAGCTATTGCTCGCCCTCCTCGCCAGCGCCGTATCCCTGGCCGCCAGCGCCGACGAGGTGCA
GGTCGCCGTCGCGGCCAACTTCACCGCGCCGATCCAGGCCATCGCCAAGGAATTCGAGAAAGACACCGGGCACAGGCTGG
TCGCCGCCTACGGCGCCACCGGCCAGTTCTATACGCAGATCAAGAACGGCGCGCCGTTCCAGGTGTTCCTCTCCGCCGAC
GACAGCACTCCGGCGAAACTGGAGCAGGAAGGCGAGGTCGTGCCCGGCTCGCGCTTCACCTATGCCATCGGCACCCTGGC
ACTCTGGTCGCCCAAGGCCGGCTATGTCGACGCCGAGGGCGAGGTGCTGAAGAGCGGCAGCTTCAGGCACCTGTCCATCG
CCAACCCGAAGACCGCGCCCTACGGCCTCGCCGCCACCCAGGCGATGGACAAGCTCGGCCTCGCCGCCACGCTCGGGCCG
AAGCTGGTGGAAGGCCAGAACATCAGCCAGGCCTACCAGTTCGTTTCCAGCGGCAACGCCGAACTGGGCTTCGTCGCTCT
GTCGCAGATCTACAAGGATGGTAAAGTCGCAACCGGCTCGGCCTGGATCGTTCCCACCGAGCTGCACGACCCGATCCGCC
AGGACGCGGTCATCCTCAACAAGGGCAAGGACAACGCCGCCGCCAAGGCCCTGGTCGACTACCTGAAGGGCGCCAAGGCC
GCCGCGCTGATCAAGTCCTACGGCTACGAACTCTAA

                                