Detailed information of regulator
Summary
| Regulator ID | REG0062  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (114 a.a.); Nucleotide sequence (342 bp) |
| Description | The expression of H2-T6SS machinery genes was controlled at a transcriptional level by QslA. |
| Reference |
External database links
| Locus tag (Gene) | PA1244 (QslA) |
| Coordinates (Strand) | 1348060..1348401 (-) |
| NCBI ID | 15596441 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I494_PSEAE |
| KEGG ID | pae:PA1244 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 4NG2 |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| H2-T6SS (PA1656) promotor | ↑ | 15596853 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| QslA_E | PF18226.3 | 1.5e-37 | 36..106 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-113 | VTLRNGVPSMTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAFGRDSLPTPMQKTAFEVAFLTRLQQRLVAARRSG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9867 | - | - |
Protein sequence of regulator: 114 a.a. .
>REG0062 NC_002516:c1348401-1348060 [Pseudomonas aeruginosa PAO1]
VTLRNGVPSMTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAF
GRDSLPTPMQKTAFEVAFLTRLQQRLVAARRSG*
VTLRNGVPSMTKDEKEKTHVDAIIERYKDLMVEIPPADRQPGLSLLWPVPAQPAIDKGVRQAENWLADQIEGQLWTAFAF
GRDSLPTPMQKTAFEVAFLTRLQQRLVAARRSG*
Nucleotide sequence of regulator: 342 bp .
>REG0062 NC_002516:c1348401-1348060 [Pseudomonas aeruginosa PAO1]
GTGACTCTCAGAAACGGAGTACCCAGCATGACGAAGGATGAAAAGGAAAAGACCCACGTCGACGCAATCATCGAACGCTA
CAAGGACCTGATGGTCGAGATTCCCCCCGCCGACCGGCAACCCGGTCTCTCCCTGCTCTGGCCGGTTCCCGCCCAGCCCG
CCATCGACAAGGGCGTCCGCCAGGCCGAGAACTGGCTTGCCGACCAGATCGAAGGCCAGCTCTGGACCGCCTTCGCCTTC
GGCCGCGACAGCCTGCCCACACCGATGCAGAAGACCGCCTTCGAAGTGGCCTTCCTGACGCGCCTGCAGCAGCGCCTGGT
AGCCGCTCGACGTTCCGGTTGA
GTGACTCTCAGAAACGGAGTACCCAGCATGACGAAGGATGAAAAGGAAAAGACCCACGTCGACGCAATCATCGAACGCTA
CAAGGACCTGATGGTCGAGATTCCCCCCGCCGACCGGCAACCCGGTCTCTCCCTGCTCTGGCCGGTTCCCGCCCAGCCCG
CCATCGACAAGGGCGTCCGCCAGGCCGAGAACTGGCTTGCCGACCAGATCGAAGGCCAGCTCTGGACCGCCTTCGCCTTC
GGCCGCGACAGCCTGCCCACACCGATGCAGAAGACCGCCTTCGAAGTGGCCTTCCTGACGCGCCTGCAGCAGCGCCTGGT
AGCCGCTCGACGTTCCGGTTGA