SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00314
Cognate effector	EFF01463
Related T6SS (Type)	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (381 a.a.); Nucleotide sequence (1143 bp)
Description	-
Reference	[1] Jiang F, Wang X, Wang B, et al. The Pseudomonas aeruginosa Type VI Secretion PGAP1-like Effector Induces Host Autophagy by Activating Endoplasmic Reticulum Stress. Cell Rep. 2016 Aug 9;16(6):1502-1509. doi: 10.1016/j.celrep.2016.07.012. Epub 2016 Jul 28. PMID: 27477276

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA1509 (TplEi)
Coordinates (Strand)	1638652..1639794 (-)
NCBI ID	15596706
RefSeq	NC_002516
Uniprot ID	Q9I3K3_PSEAE
KEGG ID	pae:PA1509
PDB ID	4R1D, 5H7Y, 5H7Z

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tli4_C	PF18426.3	2.7e-43	172..332
Tli4_N	PF18443.3	3.8e-09	40..136

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-380	MMHPRRLLAVLGVVVLTSMTSCTSFSSSRSSSMDKTGWITHCFGRFLIDLPPDAVINAGYYLWGDRIEYLDDKPTELAARVDRLEQEWRTQRHKSKGNMFLRKIDFGNESVGLLSWSSEVASKTYLLDTYVTSKPTWHVYRWKGKVSVDREQHAVEISRALARNLRSRAPKEIPSEPGFCIDHAYIAGDSFQVERFGVGVTFPEHPGARFEFRSSTGAELNSLLERVDGFVQNMLSTFAGMETLRKGKHPVGSLPGEEYLVAGSDKGQRGYTFMWEVQGKEESLTEPNLTAGLAVLERSNENGKPPPPAFKSDKEALELWDTIVDSIRVRPTSSSPRGGNAGPSPAPKPATPGGQTLGDHYVYEEFLSSLKPKDSWLDDL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9967	pos: 21-22. MTS-CT.	MMHPRRLLAVLGVVVLTSMTS

Protein sequence: 381 a.a. .

                                    >IMU00314 NC_002516:c1639794-1638652 [Pseudomonas aeruginosa PAO1]
MMHPRRLLAVLGVVVLTSMTSCTSFSSSRSSSMDKTGWITHCFGRFLIDLPPDAVINAGYYLWGDRIEYLDDKPTELAAR
VDRLEQEWRTQRHKSKGNMFLRKIDFGNESVGLLSWSSEVASKTYLLDTYVTSKPTWHVYRWKGKVSVDREQHAVEISRA
LARNLRSRAPKEIPSEPGFCIDHAYIAGDSFQVERFGVGVTFPEHPGARFEFRSSTGAELNSLLERVDGFVQNMLSTFAG
METLRKGKHPVGSLPGEEYLVAGSDKGQRGYTFMWEVQGKEESLTEPNLTAGLAVLERSNENGKPPPPAFKSDKEALELW
DTIVDSIRVRPTSSSPRGGNAGPSPAPKPATPGGQTLGDHYVYEEFLSSLKPKDSWLDDL*

                                

Nucleotide sequence: 1143 bp .

                                    >IMU00314 NC_002516:c1639794-1638652 [Pseudomonas aeruginosa PAO1]
ATGATGCATCCCCGTCGGTTGCTTGCTGTACTTGGAGTGGTGGTCTTGACCAGCATGACCAGTTGCACTTCGTTTTCTTC
TTCTCGGAGCTCTTCCATGGACAAGACCGGATGGATTACTCATTGCTTTGGTCGCTTTCTGATTGATCTGCCACCTGACG
CGGTAATCAATGCCGGATACTACCTATGGGGAGACAGAATTGAGTACTTAGATGATAAACCTACCGAACTGGCAGCCAGG
GTTGATCGTCTTGAACAAGAGTGGAGGACTCAGAGACATAAATCCAAAGGTAACATGTTTCTTCGTAAAATTGACTTTGG
CAATGAATCAGTAGGTTTATTGTCTTGGTCTTCAGAGGTTGCCTCTAAAACCTATTTATTGGATACCTATGTTACATCTA
AGCCTACGTGGCATGTCTATCGTTGGAAAGGGAAAGTTTCGGTAGACAGAGAGCAACATGCTGTGGAGATATCTAGGGCT
TTAGCTCGAAATCTACGTTCTCGCGCGCCAAAAGAAATCCCCAGCGAGCCGGGCTTTTGCATCGACCACGCTTATATTGC
AGGGGATAGTTTTCAAGTGGAGAGGTTTGGAGTGGGAGTCACTTTCCCTGAGCACCCCGGCGCGCGCTTTGAATTCCGCT
CCTCCACGGGAGCTGAGCTAAACAGCCTTCTTGAGCGAGTCGACGGCTTCGTGCAAAACATGCTCTCCACGTTTGCAGGT
ATGGAAACATTAAGAAAAGGAAAGCATCCTGTCGGGAGCCTTCCCGGAGAGGAGTATCTGGTTGCGGGAAGCGATAAGGG
GCAGCGCGGCTATACCTTCATGTGGGAGGTTCAAGGCAAGGAGGAATCGCTAACCGAGCCCAATCTTACTGCGGGACTGG
CGGTACTGGAACGCAGCAATGAAAACGGTAAACCACCGCCCCCTGCCTTCAAATCGGATAAAGAAGCATTGGAACTGTGG
GACACCATTGTCGATTCGATTCGCGTGCGCCCGACGTCTTCATCGCCACGTGGCGGAAATGCCGGTCCTTCCCCTGCTCC
GAAACCCGCTACGCCTGGCGGCCAAACGCTCGGTGATCACTATGTCTATGAGGAATTCCTCTCCAGCCTGAAGCCAAAAG
ACAGCTGGCTGGACGACCTATAG

                                