Detailed information of effector
Summary
| Effector ID | EFF01823  |
| Effector type | Tie |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (149 a.a.); Nucleotide sequence (447 bp) |
| Description | PA4922 (Azu) and ClpV2 are involved in Cu2+ uptake |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA4922 (Azu) |
| Coordinates (Strand) | 5521664..5522110 (-) |
| NCBI ID | 15600115 |
| RefSeq | NC_002516 |
| Uniprot ID | AZUR_PSEAE |
| KEGG ID | pae:PA4922 |
| PDB ID | 1XB8, 2AZU, 3FQY, 1E67, 3IN0, 3OQR, 4HZ1, 3FQ2, 3IN2, 1JZG, 1AZU, 3N2J, 1JZH, 1XB3, 3FS9, 3JTB, 2XV2, 2XV3, 4JKN, 1R1C, 1JVO, 3UGE, 1JZE, 4HHW, 1ETJ, 2FT6, 3FPY, 3IBO, 3U25, 1E5Z, 2GHZ, 4KOC, 1JZI, 4KO9, 6MJS, 2I7S, 6MJR, 2HX9, 4AZU, 2OJ1, 3NP3, 4HIP, 1E5Y, 2FT8, 2GI0, 1BEX, 1GR7, 1ILS, 2FT7, 5YT7, 1CC3, 2TSA, 1I53, 2HXA, 6GYI, 1JVL, 6MJT, 5I26, 1NZR, 2XV0, 3FSZ, 4KO7, 4QLW, 1JZJ, 3AZU, 6IAV, 2HX7, 3NP4, 4MFH, 4BWW, 1AZN, 4K9J, 2TSB, 2IDF, 3FQ1, 4HHG, 4WKX, 1AG0, 1JZF, 2FNW, 2IWE, 5I28, 1ILU, 3FSV, 1AZR, 5SYD, 4KOB, 5AZU, 1VLX, 1XB6, 1E65, 1EZL, 3FSA, 4KO6, 2FTA, 2HX8, 3FT0, 3FSW, 4KO5, 4QKT, 3JT2, 2I7O |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Copper-bind | PF00127.22 | 3e-32 | 21..148 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-148 | MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIAHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9368 | pos: 20-21. LLA-AE. | MLRKLAAVSLLSLLSAPLLA |
Protein sequence: 149 a.a. .
>EFF01823 NC_002516:c5522110-5521664 Type VI Metal Ion acquisition Effector (Tie) [Pseudomonas aeruginosa PAO1]
MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVV
TDGMASGLDKDYLKPDDSRVIAHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK*
MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDKSCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVV
TDGMASGLDKDYLKPDDSRVIAHTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK*
Nucleotide sequence: 447 bp .
>EFF01823 NC_002516:c5522110-5521664 Type VI Metal Ion acquisition Effector (Tie) [Pseudomonas aeruginosa PAO1]
ATGCTACGTAAACTCGCTGCGGTATCCCTGCTGTCCCTGCTCAGTGCGCCACTGCTGGCTGCCGAGTGCTCGGTGGACAT
CCAGGGTAACGACCAGATGCAGTTCAACACCAATGCCATCACCGTCGACAAGAGCTGCAAGCAGTTCACCGTCAACCTGT
CCCACCCCGGCAACCTGCCGAAGAACGTCATGGGCCACAACTGGGTACTGAGCACCGCCGCCGACATGCAGGGCGTGGTC
ACCGACGGCATGGCTTCCGGCCTGGACAAGGATTACCTGAAGCCCGACGACAGCCGTGTCATCGCCCACACCAAGCTGAT
CGGCTCGGGCGAGAAGGACTCGGTGACCTTCGACGTCTCCAAGCTGAAGGAAGGCGAGCAGTACATGTTCTTCTGCACCT
TCCCGGGCCACTCCGCGCTGATGAAGGGCACCCTGACCCTGAAGTGA
ATGCTACGTAAACTCGCTGCGGTATCCCTGCTGTCCCTGCTCAGTGCGCCACTGCTGGCTGCCGAGTGCTCGGTGGACAT
CCAGGGTAACGACCAGATGCAGTTCAACACCAATGCCATCACCGTCGACAAGAGCTGCAAGCAGTTCACCGTCAACCTGT
CCCACCCCGGCAACCTGCCGAAGAACGTCATGGGCCACAACTGGGTACTGAGCACCGCCGCCGACATGCAGGGCGTGGTC
ACCGACGGCATGGCTTCCGGCCTGGACAAGGATTACCTGAAGCCCGACGACAGCCGTGTCATCGCCCACACCAAGCTGAT
CGGCTCGGGCGAGAAGGACTCGGTGACCTTCGACGTCTCCAAGCTGAAGGAAGGCGAGCAGTACATGTTCTTCTGCACCT
TCCCGGGCCACTCCGCGCTGATGAAGGGCACCCTGACCCTGAAGTGA