SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00012
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Description	Hcp
Reference	[1] Han Y, Wang T, Chen G, et al. A Pseudomonas aeruginosa type VI secretion system regulated by CueR facilitates copper acquisition. PLoS Pathog. 2019 Dec 2;15(12):e1008198. doi: 10.1371/journal.ppat.1008198. eCollection 2019 Dec. PMID: 31790504

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA0263 (hcp2)
Coordinates (Strand)	297043..297561 (-)
NCBI ID	15595460
RefSeq	NC_002516
Uniprot ID	MAJE_PSEAE
KEGG ID	pae:PA0263, pae:PA1512, pae:PA5267
PDB ID	3HE1

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	6.5e-39	6..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MATPAYMSITGTKQGLITAGAFTEDSVGNTYQEGHEDQVMVQGFNHEVIIPRDPQSGQPTGQRVHKPVVITKVFDKASPLLLAALTSGERLTKVEIQWYRTSAAGTQEHYYTTVLEDAIIVDIKDYMHNCQDPGNAHFTHLEDVHFTYRKITWTHEVSGTSGSDDWRSPVAG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9168	-	-

Protein sequence: 173 a.a. .

                                    >EFF00012 NC_002516:c297561-297043  (Unknown) [Pseudomonas aeruginosa PAO1]
MATPAYMSITGTKQGLITAGAFTEDSVGNTYQEGHEDQVMVQGFNHEVIIPRDPQSGQPTGQRVHKPVVITKVFDKASPL
LLAALTSGERLTKVEIQWYRTSAAGTQEHYYTTVLEDAIIVDIKDYMHNCQDPGNAHFTHLEDVHFTYRKITWTHEVSGT
SGSDDWRSPVAG*

                                

Nucleotide sequence: 519 bp .

                                    >EFF00012 NC_002516:c297561-297043  (Unknown) [Pseudomonas aeruginosa PAO1]
ATGGCAACCCCCGCCTATATGTCCATCACCGGCACCAAGCAGGGCCTGATCACCGCCGGCGCCTTCACCGAGGACTCGGT
CGGCAACACCTATCAGGAAGGCCACGAGGACCAGGTGATGGTCCAGGGCTTCAACCACGAAGTGATCATCCCGCGCGACC
CGCAGTCCGGCCAGCCGACCGGCCAGCGCGTGCACAAGCCGGTGGTCATCACCAAGGTCTTCGACAAGGCCTCGCCGCTG
CTGCTGGCCGCCCTGACCTCCGGCGAGCGCCTGACCAAGGTCGAGATCCAGTGGTACCGCACCTCCGCCGCCGGCACCCA
GGAGCACTACTACACCACCGTCCTGGAAGACGCGATCATCGTCGACATCAAGGACTACATGCACAACTGCCAGGACCCGG
GCAACGCCCACTTCACCCACCTGGAAGACGTGCACTTCACCTACCGCAAGATCACCTGGACCCACGAAGTCTCCGGCACC
TCCGGCTCCGACGACTGGCGCTCGCCGGTGGCAGGGTGA

                                