Detailed information of effector
Summary
| Effector ID | EFF01892  |
| Effector type | Tse |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (213 a.a.); Nucleotide sequence (639 bp) |
| Description | Copurification experiments validated the interaction between PA2066 and Hcp2 (10-fold enrichment),PA0256 and Hcp3 (8.4-fold enrichment). PA2066 and PA0256 are bona fide Hcp cargoes and might be H2- and H3-T6SS toxins with antibacterial activity, respectively. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA2066 |
| Coordinates (Strand) | 2265126..2265764 (-) |
| NCBI ID | 15597262 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I249_PSEAE |
| KEGG ID | pae:PA2066 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF218 | PF02698.19 | 2.5e-14 | 27..131 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-212 | MSPTLQDARLLWDFLGQGRCHVPCELLVVCGSYDLRVCDHACELLERGLAERLLFTGNTGHWTRHLWVEPEAELFARHARARGVSDSLIVIEPRATNFAENIAFARELFPDLRRATFVTKPNSIRRVALTLPVRWPGLEGHVDAPDYAFPDGISNLVGVLGVIDEMVGDLDRIMRYPALGFQVACPIPAQVMDAWERLVARGFDRHLVNPPR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9726 | - | - |
Protein sequence: 213 a.a. .
>EFF01892 NC_002516:c2265764-2265126 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAO1]
MSPTLQDARLLWDFLGQGRCHVPCELLVVCGSYDLRVCDHACELLERGLAERLLFTGNTGHWTRHLWVEPEAELFARHAR
ARGVSDSLIVIEPRATNFAENIAFARELFPDLRRATFVTKPNSIRRVALTLPVRWPGLEGHVDAPDYAFPDGISNLVGVL
GVIDEMVGDLDRIMRYPALGFQVACPIPAQVMDAWERLVARGFDRHLVNPPR*
MSPTLQDARLLWDFLGQGRCHVPCELLVVCGSYDLRVCDHACELLERGLAERLLFTGNTGHWTRHLWVEPEAELFARHAR
ARGVSDSLIVIEPRATNFAENIAFARELFPDLRRATFVTKPNSIRRVALTLPVRWPGLEGHVDAPDYAFPDGISNLVGVL
GVIDEMVGDLDRIMRYPALGFQVACPIPAQVMDAWERLVARGFDRHLVNPPR*
Nucleotide sequence: 639 bp .
>EFF01892 NC_002516:c2265764-2265126 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAO1]
ATGAGCCCGACCCTGCAAGATGCCCGGCTGCTCTGGGACTTCCTCGGCCAGGGCCGCTGCCATGTGCCCTGCGAACTGCT
GGTGGTGTGCGGCTCCTACGACCTGCGGGTCTGCGACCACGCCTGCGAACTGCTCGAACGCGGCCTCGCCGAACGCCTGC
TGTTCACCGGCAACACCGGGCACTGGACGCGCCATCTCTGGGTCGAGCCGGAAGCCGAGCTGTTCGCCCGCCACGCCAGG
GCACGCGGGGTAAGCGACAGCCTCATCGTGATCGAGCCGCGGGCGACCAACTTCGCCGAGAACATCGCCTTCGCCCGCGA
GCTGTTCCCCGACCTGCGCCGCGCGACCTTCGTCACCAAGCCCAACTCGATCCGCCGCGTGGCGCTGACCCTGCCGGTCC
GCTGGCCGGGTCTGGAGGGACACGTGGATGCGCCCGACTACGCATTTCCCGACGGCATCTCGAACCTGGTCGGCGTACTC
GGGGTGATCGACGAGATGGTCGGCGACCTTGACCGCATCATGCGTTACCCGGCGCTGGGCTTCCAGGTCGCCTGCCCGAT
CCCGGCGCAGGTCATGGACGCCTGGGAACGCCTGGTGGCGCGCGGTTTCGACCGCCACCTGGTGAACCCGCCGCGCTGA
ATGAGCCCGACCCTGCAAGATGCCCGGCTGCTCTGGGACTTCCTCGGCCAGGGCCGCTGCCATGTGCCCTGCGAACTGCT
GGTGGTGTGCGGCTCCTACGACCTGCGGGTCTGCGACCACGCCTGCGAACTGCTCGAACGCGGCCTCGCCGAACGCCTGC
TGTTCACCGGCAACACCGGGCACTGGACGCGCCATCTCTGGGTCGAGCCGGAAGCCGAGCTGTTCGCCCGCCACGCCAGG
GCACGCGGGGTAAGCGACAGCCTCATCGTGATCGAGCCGCGGGCGACCAACTTCGCCGAGAACATCGCCTTCGCCCGCGA
GCTGTTCCCCGACCTGCGCCGCGCGACCTTCGTCACCAAGCCCAACTCGATCCGCCGCGTGGCGCTGACCCTGCCGGTCC
GCTGGCCGGGTCTGGAGGGACACGTGGATGCGCCCGACTACGCATTTCCCGACGGCATCTCGAACCTGGTCGGCGTACTC
GGGGTGATCGACGAGATGGTCGGCGACCTTGACCGCATCATGCGTTACCCGGCGCTGGGCTTCCAGGTCGCCTGCCCGAT
CCCGGCGCAGGTCATGGACGCCTGGGAACGCCTGGTGGCGCGCGGTTTCGACCGCCACCTGGTGAACCCGCCGCGCTGA