Detailed information of accessory protein


Summary

Accessory protein ID ACP00044 experimental
Type chaperone
Related T6SS (Type) T6SS00004 (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Related structure protein -
Related effector PA1863 (ModA)
Description ModA binds to molybdate in the periplasm and ModBC harbors two integral membrane permeases.
Reference [1] Wang T, Du X, Ji L, et al. Pseudomonas aeruginosa T6SS-mediated molybdate transport contributes to bacterial competition during anaerobiosis. Cell Rep. 2021 Apr 13;35(2):108957. doi: 10.1016/j.celrep.2021.108957. PMID: 33852869; [2] Pederick VG, Eijkelkamp BA, Ween MP, et al. Acquisition and role of molybdate in Pseudomonas aeruginosa. Appl Environ Microbiol. 2014 Nov;80(21):6843-52. doi: 10.1128/AEM.02465-14. Epub 2014 Aug 29. PMID: 25172858; [3] Périnet S, Jeukens J, Kukavica-Ibrulj I, et al. Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection. BMC Res Notes. 2016 Jan 12;9:23. doi: 10.1186/s13104-016-1840-x. PMID: 26758577
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) PA1862 (ModB)
Coordinates (Strand) 2021712..2022398 (-)
NCBI ID 15597059
RefSeq NC_002516
Uniprot ID Q9I2N3_PSEAE
KEGG ID pae:PA1862
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
BPD_transp_1 PF00528.24 4.5e-18 31..217
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-14 MALPLDSTDLAAIW
Transmembrane helix 15-37 LTIELASLTTLLLLLIGTPIAWW
Inside 38-48 LARTRSWLKGP
Transmembrane helix 49-71 IGAVVALPLVLPPTVIGFYLLVS
Outside 72-85 MGPNGFIGQLTQSL
Transmembrane helix 86-108 GLGTLPFTFAGLVVGSVFYSLPF
Inside 109-138 VVQPLQNAFEAIGEKPLEAAATLRASPVDR
Transmembrane helix 139-161 FFSVVLPLARPGFVTAAILGFAH
Outside 162-196 TVGEFGVVLMIGGNIPEKTRVVSVQIFDHVEAMEY
Transmembrane helix 197-219 AQAHWLAGGMVAFSFVVLLALYS
Inside 220-228 GRRRRPGWA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9988 - -


  Protein sequence: 229 a.a.    

>ACP00044 NC_002516:c2022398-2021712 [Pseudomonas aeruginosa PAO1]
MALPLDSTDLAAIWLTIELASLTTLLLLLIGTPIAWWLARTRSWLKGPIGAVVALPLVLPPTVIGFYLLVSMGPNGFIGQ
LTQSLGLGTLPFTFAGLVVGSVFYSLPFVVQPLQNAFEAIGEKPLEAAATLRASPVDRFFSVVLPLARPGFVTAAILGFA
HTVGEFGVVLMIGGNIPEKTRVVSVQIFDHVEAMEYAQAHWLAGGMVAFSFVVLLALYSGRRRRPGWA*

  Nucleotide sequence: 687 bp    

>ACP00044 NC_002516:c2022398-2021712 [Pseudomonas aeruginosa PAO1]
ATGGCGTTGCCACTCGACAGTACGGACCTGGCGGCCATCTGGCTGACCATCGAGCTGGCTTCGCTCACCACCCTCCTCCT
GCTGCTGATCGGCACGCCCATCGCCTGGTGGCTGGCGCGCACCCGCTCCTGGCTGAAGGGGCCGATCGGCGCGGTGGTGG
CCCTGCCGCTGGTGCTGCCGCCGACGGTGATCGGCTTCTACCTGCTGGTCAGCATGGGTCCCAACGGTTTCATCGGCCAG
CTCACCCAGAGCCTCGGTCTCGGCACCCTGCCCTTCACCTTCGCCGGTCTGGTGGTCGGTTCGGTGTTCTACTCGCTGCC
CTTCGTCGTGCAACCGTTGCAGAACGCCTTCGAGGCGATCGGCGAGAAACCGCTGGAGGCTGCCGCGACCCTGCGCGCGA
GCCCCGTCGACCGTTTCTTCAGCGTGGTCCTGCCGCTGGCCCGGCCGGGTTTCGTCACCGCCGCGATCCTCGGCTTCGCC
CATACCGTCGGCGAGTTCGGCGTGGTGCTGATGATCGGCGGCAACATCCCGGAGAAAACCCGCGTGGTCTCGGTGCAGAT
ATTCGATCACGTCGAAGCCATGGAATACGCCCAGGCCCACTGGCTGGCCGGCGGCATGGTGGCGTTCTCCTTCGTCGTCC
TCCTCGCCCTCTATTCGGGCCGGCGCCGCCGGCCGGGCTGGGCCTGA