Detailed information of regulator
Summary
| Regulator ID | REG0068  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (135 a.a.); Nucleotide sequence (405 bp) |
| Description | The expression of the plasmid borne transcriptional fusion was studied in an E. coli WT and isogenic fur mutant and the activity of the fusion increased by 2.6-fold in the Fur mutant. |
| Reference |
External database links
| Locus tag (Gene) | PA4764 (Fur) |
| Coordinates (Strand) | 5351675..5352079 (-) |
| NCBI ID | 15599958 |
| RefSeq | NC_002516 |
| Uniprot ID | FUR_PSEAE |
| KEGG ID | pae:PA4764 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 6H1C, 1MZB |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| H2-T6SS Transcription | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FUR | PF01475.21 | 2.3e-46 | 9..129 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-134 | MVENSELRKAGLKVTLPRVKILQMLDSAEQRHMSAEDVYKALMEAGEDVGLATVYRVLTQFEAAGLVVRHNFDGGHAVFELADSGHHDHMVCVDTGEVIEFMDAEIEKRQKEIVRERGFELVDHNLVLYVRKKK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9978 | - | - |
Protein sequence of regulator: 135 a.a. .
>REG0068 NC_002516:c5352079-5351675 [Pseudomonas aeruginosa PAO1]
MVENSELRKAGLKVTLPRVKILQMLDSAEQRHMSAEDVYKALMEAGEDVGLATVYRVLTQFEAAGLVVRHNFDGGHAVFE
LADSGHHDHMVCVDTGEVIEFMDAEIEKRQKEIVRERGFELVDHNLVLYVRKKK*
MVENSELRKAGLKVTLPRVKILQMLDSAEQRHMSAEDVYKALMEAGEDVGLATVYRVLTQFEAAGLVVRHNFDGGHAVFE
LADSGHHDHMVCVDTGEVIEFMDAEIEKRQKEIVRERGFELVDHNLVLYVRKKK*
Nucleotide sequence of regulator: 405 bp .
>REG0068 NC_002516:c5352079-5351675 [Pseudomonas aeruginosa PAO1]
ATGGTTGAAAATAGCGAACTTCGAAAAGCCGGCCTTAAAGTGACCCTGCCGCGGGTCAAGATCCTGCAGATGCTCGACTC
GGCCGAGCAACGCCACATGAGCGCCGAAGACGTGTACAAGGCGCTGATGGAAGCAGGCGAGGACGTGGGCCTGGCAACCG
TCTATCGGGTGCTGACCCAGTTCGAGGCCGCCGGCCTGGTGGTGCGTCACAACTTCGATGGCGGCCATGCCGTGTTCGAG
CTCGCCGATAGCGGCCACCACGACCACATGGTCTGCGTCGATACCGGCGAGGTGATCGAGTTCATGGATGCGGAAATCGA
GAAGCGCCAGAAGGAAATCGTCCGCGAGCGCGGCTTCGAGCTGGTCGATCACAATCTGGTGCTCTACGTGCGCAAGAAGA
AGTAG
ATGGTTGAAAATAGCGAACTTCGAAAAGCCGGCCTTAAAGTGACCCTGCCGCGGGTCAAGATCCTGCAGATGCTCGACTC
GGCCGAGCAACGCCACATGAGCGCCGAAGACGTGTACAAGGCGCTGATGGAAGCAGGCGAGGACGTGGGCCTGGCAACCG
TCTATCGGGTGCTGACCCAGTTCGAGGCCGCCGGCCTGGTGGTGCGTCACAACTTCGATGGCGGCCATGCCGTGTTCGAG
CTCGCCGATAGCGGCCACCACGACCACATGGTCTGCGTCGATACCGGCGAGGTGATCGAGTTCATGGATGCGGAAATCGA
GAAGCGCCAGAAGGAAATCGTCCGCGAGCGCGGCTTCGAGCTGGTCGATCACAATCTGGTGCTCTACGTGCGCAAGAAGA
AGTAG