Detailed information of regulator


Summary

Regulator ID REG0066 experimental
Regulator type protein
Regulatory pathway (from literatures) QS regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Cysteine and methionine metabolism (map00270); Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00004 experimental (Type i1)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (202 a.a.); Nucleotide sequence (606 bp)
Description The addition of the C4-HSL (10 μm) to the rhlI mutant restored induction of the expression at the transition phase of growth, and the expression was 4-fold higher than in the mutant. And upon RhlR and RhlI production induced by IPTG, a 3.3-fold up-regulation of the H2-T6SS promoter region was observed.
Reference
External database links

Locus tag (Gene) PA3476 (rhlI)
Coordinates (Strand) 3889139..3889744 (-)
NCBI ID 15598672
RefSeq NC_002516
Uniprot ID RHLI_PSEAE
KEGG ID pae:PA3476
KEGG pathway (determined by BlastKOALA) map01100; map00270; map02020; map02024; map02025
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
PA3477 (RhlR)↓15598673
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
Autoind_synthPF00765.191e-1912..179
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-201MIELLSESLEGLSAAMIAELGRYRHQVFIEKLGWDVVSTSRVRDQEFDQFDHPQTRYIVAMSRQGICGCARLLPTTDAYLLKDVFAYLCSETPPSDPSVWELSRYAASAADDPQLAMKIFWSSLQCAWYLGASSVVAVTTTAMERYFVRNGVILQRLGPPQKVKGETLVAISFPAYQERGLEMLLRYHPEWLQGVPLSMAV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9887--


  Protein sequence of regulator: 202 a.a.    .

>REG0066 NC_002516:c3889744-3889139 [Pseudomonas aeruginosa PAO1]
MIELLSESLEGLSAAMIAELGRYRHQVFIEKLGWDVVSTSRVRDQEFDQFDHPQTRYIVAMSRQGICGCARLLPTTDAYL
LKDVFAYLCSETPPSDPSVWELSRYAASAADDPQLAMKIFWSSLQCAWYLGASSVVAVTTTAMERYFVRNGVILQRLGPP
QKVKGETLVAISFPAYQERGLEMLLRYHPEWLQGVPLSMAV*

  Nucleotide sequence of regulator: 606 bp    .

>REG0066 NC_002516:c3889744-3889139 [Pseudomonas aeruginosa PAO1]
ATGATCGAATTGCTCTCTGAATCGCTGGAAGGGCTTTCCGCCGCCATGATCGCCGAGCTGGGACGCTACCGGCATCAGGT
CTTCATCGAGAAGCTGGGCTGGGACGTGGTCTCCACCTCCAGGGTCCGCGACCAGGAATTCGACCAGTTCGACCATCCGC
AAACCCGCTACATCGTCGCCATGAGCCGCCAGGGCATCTGCGGTTGCGCCCGCCTGCTGCCGACGACCGACGCCTACCTG
CTCAAGGACGTCTTCGCCTACCTGTGCAGCGAAACCCCGCCGAGCGATCCGTCGGTCTGGGAGCTTTCGCGCTACGCCGC
CAGCGCGGCGGACGATCCGCAGCTGGCGATGAAGATATTCTGGTCCAGCCTGCAATGCGCCTGGTACCTGGGCGCCAGTT
CGGTGGTGGCGGTGACCACCACGGCCATGGAGCGCTATTTCGTTCGCAACGGCGTGATCCTCCAGCGCCTCGGCCCGCCG
CAGAAGGTCAAGGGCGAGACGCTGGTCGCGATCAGCTTCCCGGCCTACCAGGAGCGCGGCCTGGAGATGCTGCTGCGCTA
CCACCCGGAATGGCTGCAGGGCGTACCGCTGTCGATGGCGGTGTGA