Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00360  |
| Cognate effector | EFF01870 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (158 a.a.); Nucleotide sequence (474 bp) |
| Description | PA0808 is the auto-immunity protein for AmpDh3 and mitigates its self-deleterious effect |
| Reference | [1] Wang T, Hu Z, Du X, et al. A type VI secretion system delivers a cell wall amidase to target bacterial competitors. Mol Microbiol. 2020 Aug;114(2):308-321. doi: 10.1111/mmi.14513. Epub 2020 Apr 22. PMID: 32279364 [2] Speare L, Smith S, Salvato F, et al. Environmental Viscosity Modulates Interbacterial Killing during Habitat Transition. mBio. 2020 Feb 4;11(1). pii: e03060-19. doi: 10.1128/mBio.03060-19. PMID: 32019799 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA0808 |
| Coordinates (Strand) | 885635..886108 (+) |
| NCBI ID | 15596005 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I5D0_PSEAE |
| KEGG ID | pae:PA0808 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| AHSA1 | PF08327.13 | 1.9e-14 | 18..139 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-157 | MNAVAQVVAKAEMLIRRPIAEVFEAFVDPAITARFWFSRGDARLEAGKRLRWHWDMYGVSQEIEVKDLQTNRRILIEWPNGDSNPSQVEWLFEELPGAGTFVSIRNSGFVGTPEEVIPRVVDATEGFTLVLAGLKACLEHGIALNLVADRFPRGLDG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9876 | - | - |
Protein sequence: 158 a.a. .
>IMU00360 NC_002516:885635-886108 [Pseudomonas aeruginosa PAO1]
MNAVAQVVAKAEMLIRRPIAEVFEAFVDPAITARFWFSRGDARLEAGKRLRWHWDMYGVSQEIEVKDLQTNRRILIEWPN
GDSNPSQVEWLFEELPGAGTFVSIRNSGFVGTPEEVIPRVVDATEGFTLVLAGLKACLEHGIALNLVADRFPRGLDG*
MNAVAQVVAKAEMLIRRPIAEVFEAFVDPAITARFWFSRGDARLEAGKRLRWHWDMYGVSQEIEVKDLQTNRRILIEWPN
GDSNPSQVEWLFEELPGAGTFVSIRNSGFVGTPEEVIPRVVDATEGFTLVLAGLKACLEHGIALNLVADRFPRGLDG*
Nucleotide sequence: 474 bp .
>IMU00360 NC_002516:885635-886108 [Pseudomonas aeruginosa PAO1]
ATGAATGCCGTCGCACAGGTCGTCGCCAAGGCGGAAATGCTGATCCGCCGCCCCATCGCCGAGGTCTTCGAAGCCTTCGT
CGATCCGGCCATCACCGCCAGGTTCTGGTTCAGCCGCGGCGATGCCCGCCTCGAAGCCGGGAAACGGCTGCGCTGGCACT
GGGACATGTACGGGGTTTCCCAGGAAATCGAAGTGAAGGACCTGCAGACGAACCGCCGCATCCTCATCGAATGGCCGAAT
GGCGACAGCAACCCTTCCCAGGTGGAGTGGCTGTTCGAGGAACTGCCCGGCGCCGGGACCTTCGTCAGCATCCGCAACAG
CGGTTTCGTCGGCACCCCGGAAGAGGTCATCCCGCGGGTGGTCGACGCCACCGAAGGTTTCACCCTGGTGCTGGCCGGGC
TCAAGGCCTGCCTGGAGCATGGCATCGCCTTGAACCTGGTGGCCGACCGTTTTCCCCGCGGTCTCGATGGCTGA
ATGAATGCCGTCGCACAGGTCGTCGCCAAGGCGGAAATGCTGATCCGCCGCCCCATCGCCGAGGTCTTCGAAGCCTTCGT
CGATCCGGCCATCACCGCCAGGTTCTGGTTCAGCCGCGGCGATGCCCGCCTCGAAGCCGGGAAACGGCTGCGCTGGCACT
GGGACATGTACGGGGTTTCCCAGGAAATCGAAGTGAAGGACCTGCAGACGAACCGCCGCATCCTCATCGAATGGCCGAAT
GGCGACAGCAACCCTTCCCAGGTGGAGTGGCTGTTCGAGGAACTGCCCGGCGCCGGGACCTTCGTCAGCATCCGCAACAG
CGGTTTCGTCGGCACCCCGGAAGAGGTCATCCCGCGGGTGGTCGACGCCACCGAAGGTTTCACCCTGGTGCTGGCCGGGC
TCAAGGCCTGCCTGGAGCATGGCATCGCCTTGAACCTGGTGGCCGACCGTTTTCCCCGCGGTCTCGATGGCTGA