SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01496
Effector type	Tpe
Cognate immunity protein	IMU00315
Related T6SS (Type)	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (1020 a.a.); Nucleotide sequence (3060 bp)
Description	VgrG2b is a novel antibacterial protease active in the periplasm, and VgrG2bC-ter metallopeptidase represents a hitherto-undescribed family of antibacterial T6SS effectors that appear to dysregulate the cell division process
Reference	[1] Wood TE, Howard SA, Förster A, et al. The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology. Cell Rep. 2019 Oct 1;29(1):187-201.e7. doi: 10.1016/j.celrep.2019.08.094. PMID: 31577948 [2] Berni B, Soscia C, Djermoun S, et al. A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa. Front Microbiol. 2019 Jun 5;10:1218. doi: 10.3389/fmicb.2019.01218. eCollection 2019. PMID: 31231326

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA0262 (VgrG2b)
Coordinates (Strand)	293802..296861 (-)
NCBI ID	15595459
RefSeq	NC_002516
Uniprot ID	VGR2B_PSEAE
KEGG ID	pae:PA0262
PDB ID	6H56

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	5.6e-75	27..327
DUF2345	PF10106.11	4.8e-48	615..758
T6SS_Vgr	PF13296.8	2.2e-34	475..581
Phage_base_V	PF04717.14	9.1e-14	386..453

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1019	MRQRDLKFTFVVGEGKLAFDVVEFELEEALCEPFRLNLKLASDKNAIDFKQVLDQPGTFTLWQDGRPARYVHGIVSHFTQGSSGFRRTRYELLLEPQLARLELCCNWRIFQEKSVPEILQALLKEHRVLDYEQRIYHEHLPREYCVQAGDSDHYLHDRLAFEEGLVYYFRFDEHRHTLVCSDRLYVQERIAGGPVLFSAQPEGDNPQPVLHSFRYSENVRTARQTQRDYSFKRPTYDQEHHLAGEALEHQGSSYERYDYPGRYKRSGAGRPFTESRLRGHRRDARVASVSGDDPRLIPGHAFALEGHPRADFNAWWRPVRVVHRGTQYAGQEEESADAPLGVSYDLRAELVPEDVEWRPAPLPRPRIDGPQIATVVGPAGEEIHCDEWGRVKVQFPWDREGRHDEFSTCWIRVAQNWAGADWGHMAIPRIGQEVIVDYLDGDCDQPIVTGRTYRATNRPPYALPDHKILSTIKSKEYKGSRANELRIDDTTAQISAALMSDHGASALHLGYLTHPRPEGGKPRGEGFELRTDEHGAVRAAKGLLLSTEEQLRAGAGHLDRGVVVQVLEAALELARELGDYAGEHQGVGHDAAPQQTLQEAVRDLGHGANDESGKSNGGKPAIALSGPAGIAAATPASLTLAAGEHVDSVARQNQQVTAGQKVVINAGSDIGLFAQGGELRQITHQGPMLLQAQKNDIRLEAKQSVEVSASQQHVLVTAKEHITLMCGGAYLTLKGGNIELGMPGNFVVKAAKHSHVGAASLEAELPQFEVGETQRRFVLKQLDGQTAMPNVPYTITMANGEVIEGVTDAEGATQLLQKDAMNIAKVDMKHTKSPASAVAGIAAAVGAAVAVGKLLGGPDAEAGRALSEGEISLAKGVFGDSIDYSTVRLRDEDYVPWQGKDYVMAPNGHIYFGEELRGVADWSLESLQRQGLFIHEMTHVWQHQHGVNVLLVGAYQQARQFLLGDQYAYRLEPGKTLKDYNIEQQGDIVRDYFLEKNEFGEASANSRFAGVLKNFPTGY

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9855	-	-

Protein sequence: 1020 a.a. .

                                    >EFF01496 NC_002516:c296861-293802 Type VI Peptidase Effector (Tpe) [Pseudomonas aeruginosa PAO1]
MRQRDLKFTFVVGEGKLAFDVVEFELEEALCEPFRLNLKLASDKNAIDFKQVLDQPGTFTLWQDGRPARYVHGIVSHFTQ
GSSGFRRTRYELLLEPQLARLELCCNWRIFQEKSVPEILQALLKEHRVLDYEQRIYHEHLPREYCVQAGDSDHYLHDRLA
FEEGLVYYFRFDEHRHTLVCSDRLYVQERIAGGPVLFSAQPEGDNPQPVLHSFRYSENVRTARQTQRDYSFKRPTYDQEH
HLAGEALEHQGSSYERYDYPGRYKRSGAGRPFTESRLRGHRRDARVASVSGDDPRLIPGHAFALEGHPRADFNAWWRPVR
VVHRGTQYAGQEEESADAPLGVSYDLRAELVPEDVEWRPAPLPRPRIDGPQIATVVGPAGEEIHCDEWGRVKVQFPWDRE
GRHDEFSTCWIRVAQNWAGADWGHMAIPRIGQEVIVDYLDGDCDQPIVTGRTYRATNRPPYALPDHKILSTIKSKEYKGS
RANELRIDDTTAQISAALMSDHGASALHLGYLTHPRPEGGKPRGEGFELRTDEHGAVRAAKGLLLSTEEQLRAGAGHLDR
GVVVQVLEAALELARELGDYAGEHQGVGHDAAPQQTLQEAVRDLGHGANDESGKSNGGKPAIALSGPAGIAAATPASLTL
AAGEHVDSVARQNQQVTAGQKVVINAGSDIGLFAQGGELRQITHQGPMLLQAQKNDIRLEAKQSVEVSASQQHVLVTAKE
HITLMCGGAYLTLKGGNIELGMPGNFVVKAAKHSHVGAASLEAELPQFEVGETQRRFVLKQLDGQTAMPNVPYTITMANG
EVIEGVTDAEGATQLLQKDAMNIAKVDMKHTKSPASAVAGIAAAVGAAVAVGKLLGGPDAEAGRALSEGEISLAKGVFGD
SIDYSTVRLRDEDYVPWQGKDYVMAPNGHIYFGEELRGVADWSLESLQRQGLFIHEMTHVWQHQHGVNVLLVGAYQQARQ
FLLGDQYAYRLEPGKTLKDYNIEQQGDIVRDYFLEKNEFGEASANSRFAGVLKNFPTGY*

                                

Nucleotide sequence: 3060 bp .

                                    >EFF01496 NC_002516:c296861-293802 Type VI Peptidase Effector (Tpe) [Pseudomonas aeruginosa PAO1]
ATGCGTCAAAGGGACCTGAAATTCACCTTCGTCGTCGGCGAAGGGAAACTCGCGTTCGATGTCGTCGAGTTCGAACTGGA
AGAAGCGCTCTGCGAGCCTTTCCGCCTGAACCTGAAGCTGGCCAGCGACAAGAACGCCATCGATTTCAAGCAGGTACTCG
ACCAGCCGGGCACCTTCACCCTCTGGCAGGACGGCCGTCCGGCCCGCTACGTCCATGGCATCGTCAGCCACTTCACCCAG
GGCAGCTCGGGTTTTCGCCGGACCCGCTACGAACTGCTGCTGGAACCGCAGTTGGCGCGCCTGGAGCTGTGCTGCAACTG
GCGGATCTTCCAGGAGAAGAGTGTTCCGGAGATTCTCCAGGCGCTGCTCAAGGAACACCGGGTGCTCGACTACGAACAGC
GCATCTACCATGAGCACCTGCCGCGCGAATACTGCGTGCAGGCCGGCGACAGCGATCACTACCTGCACGACCGGCTGGCT
TTCGAGGAAGGCCTGGTCTACTACTTCCGCTTCGACGAACACCGCCACACCCTGGTCTGCAGCGACCGGCTCTACGTCCA
GGAGCGTATCGCCGGCGGCCCGGTACTGTTCAGCGCCCAGCCGGAGGGCGACAACCCGCAGCCGGTGCTTCATTCTTTCC
GCTACAGCGAAAATGTTCGTACGGCTCGACAGACCCAGCGGGACTACAGCTTCAAGCGCCCGACCTACGACCAGGAGCAT
CATCTCGCTGGCGAGGCGCTCGAGCATCAGGGCTCGTCCTACGAGCGCTACGACTACCCCGGTCGCTACAAGCGGAGCGG
CGCCGGCCGGCCTTTCACCGAAAGCCGCCTCAGAGGGCACCGGCGCGATGCGCGGGTGGCATCGGTCAGCGGCGACGACC
CGCGGCTGATCCCGGGCCACGCCTTTGCCCTGGAAGGTCATCCGCGTGCCGACTTCAACGCCTGGTGGCGGCCGGTGCGG
GTGGTCCATCGCGGCACCCAGTATGCCGGCCAGGAGGAAGAGTCCGCCGACGCCCCTCTGGGGGTGAGCTACGACCTGCG
CGCCGAACTGGTACCCGAGGACGTCGAATGGCGTCCCGCACCGCTGCCTCGGCCGCGTATCGATGGTCCGCAGATCGCGA
CGGTAGTCGGCCCGGCAGGGGAAGAGATCCATTGCGACGAGTGGGGACGGGTGAAGGTCCAGTTCCCCTGGGATCGCGAG
GGACGTCACGATGAGTTCAGTACCTGCTGGATACGGGTCGCGCAGAACTGGGCAGGTGCCGACTGGGGGCACATGGCGAT
CCCGCGGATTGGCCAGGAGGTGATCGTCGATTACCTGGACGGCGACTGCGACCAGCCGATCGTCACCGGGCGCACCTACC
GGGCGACCAACCGACCGCCCTACGCGCTGCCCGACCACAAGATCCTCAGCACCATCAAGAGCAAGGAGTACAAGGGCAGT
CGGGCCAACGAGCTGCGCATCGACGACACCACGGCGCAGATCAGTGCGGCGCTGATGAGCGACCACGGTGCCAGCGCGCT
GCACCTGGGCTACCTGACCCATCCTCGGCCCGAAGGCGGCAAGCCGCGCGGCGAGGGCTTCGAACTGCGCACCGACGAAC
ATGGCGCGGTGCGTGCGGCCAAGGGGCTGCTGCTGAGCACCGAGGAGCAGCTCAGGGCCGGCGCCGGCCATCTCGACCGA
GGCGTCGTGGTGCAGGTGCTGGAGGCCGCGCTGGAGCTGGCCCGCGAGCTGGGCGACTACGCCGGGGAGCACCAGGGCGT
GGGGCACGACGCGGCGCCGCAGCAAACCCTCCAGGAAGCCGTGCGCGACCTCGGCCACGGCGCCAACGACGAGTCCGGCA
AGAGCAACGGGGGCAAGCCGGCGATCGCCTTGAGCGGTCCGGCAGGGATCGCCGCGGCGACGCCGGCGAGCCTGACCCTG
GCCGCCGGCGAGCACGTCGACAGCGTGGCCCGGCAGAACCAGCAGGTGACCGCCGGGCAGAAGGTGGTGATCAACGCCGG
CAGCGACATCGGCCTGTTCGCCCAGGGCGGCGAGCTGCGCCAGATCACCCACCAGGGGCCGATGCTGCTGCAGGCGCAGA
AGAACGACATCCGCCTGGAGGCCAAGCAGAGCGTGGAGGTCAGCGCCAGCCAGCAGCACGTGCTGGTCACCGCGAAGGAG
CACATCACCCTGATGTGCGGCGGCGCCTACCTGACCCTCAAGGGCGGCAACATCGAGCTGGGCATGCCCGGCAACTTCGT
GGTCAAGGCGGCGAAGCACAGTCATGTCGGCGCGGCCAGCCTGGAAGCCGAGCTGCCGCAGTTCGAGGTGGGCGAGACGC
AGCGGCGGTTCGTCCTCAAGCAACTGGACGGGCAAACGGCCATGCCCAACGTGCCCTATACCATCACCATGGCCAACGGC
GAGGTCATCGAGGGCGTCACCGACGCCGAAGGCGCTACCCAGCTGCTGCAGAAGGATGCGATGAACATCGCCAAGGTCGA
TATGAAACACACGAAATCGCCCGCCTCCGCAGTTGCCGGGATAGCAGCGGCGGTTGGCGCGGCGGTTGCAGTCGGCAAGC
TCCTGGGTGGCCCCGACGCCGAGGCCGGACGTGCTCTCAGCGAAGGGGAAATCAGCCTGGCGAAAGGCGTATTCGGCGAT
TCCATCGACTATTCCACGGTCCGCCTGCGCGACGAGGACTATGTCCCCTGGCAGGGCAAGGACTACGTGATGGCGCCGAA
CGGCCACATCTACTTCGGCGAGGAGTTGCGCGGCGTGGCGGACTGGAGCCTGGAGAGCCTGCAGCGCCAGGGTCTGTTCA
TTCACGAAATGACCCATGTCTGGCAGCACCAGCATGGCGTCAACGTCCTTCTGGTGGGGGCCTACCAGCAAGCCAGGCAG
TTTCTCCTAGGCGACCAGTACGCCTACCGGTTGGAGCCGGGAAAGACGTTGAAGGACTACAACATCGAACAGCAGGGAGA
CATCGTTCGAGATTATTTCCTCGAGAAGAACGAGTTCGGGGAAGCCTCTGCGAACAGCAGGTTCGCTGGCGTACTGAAAA
ACTTCCCAACGGGATACTGA

                                