Detailed information of regulator
Summary
| Regulator ID | REG0063  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Transcription regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (133 a.a.); Nucleotide sequence (399 bp) |
| Description | CueR only impacts H2-T6SS activity, through binding the sequence of tssA2 and hcp2,but not the H1- and H3-T6SSs. |
| Reference |
External database links
| Locus tag (Gene) | PA4778 (CueR) |
| Coordinates (Strand) | 5366257..5366655 (+) |
| NCBI ID | 15599972 |
| RefSeq | NC_002516 |
| Uniprot ID | Y4778_PSEAE |
| KEGG ID | pae:PA4778 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| tssA2 (-173 to -140 relative to the ATG start codon) | ↓ | AATAGGAAAATTCCCAAAGAGGGAATGTCCTGTT |
| hcp2 (-208 to -159 relative to the ATG start codon) | ↓ | TCCGAGAGAGTGCGCAACTTTTTGCAAGCGGTGCGCAAAAAGTTGCGCAA |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MerR-DNA-bind | PF09278.13 | 1.1e-20 | 44..108 |
| MerR | PF00376.25 | 5.5e-16 | 2..39 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-132 | MNIGEAAKKSGLTPKMIRYYESIELLRPAGRSASGYRHYNENDLHTLAFIRRSRDLGFSLDEVGKLLTLWQDRQRASADVKALAAQHVRELNRKIEELSTLRDTLQDLVEHCQGDHRPDCPILKDLASGCCH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9924 | - | - |
Protein sequence of regulator: 133 a.a. .
>REG0063 NC_002516:5366257-5366655 [Pseudomonas aeruginosa PAO1]
MNIGEAAKKSGLTPKMIRYYESIELLRPAGRSASGYRHYNENDLHTLAFIRRSRDLGFSLDEVGKLLTLWQDRQRASADV
KALAAQHVRELNRKIEELSTLRDTLQDLVEHCQGDHRPDCPILKDLASGCCH*
MNIGEAAKKSGLTPKMIRYYESIELLRPAGRSASGYRHYNENDLHTLAFIRRSRDLGFSLDEVGKLLTLWQDRQRASADV
KALAAQHVRELNRKIEELSTLRDTLQDLVEHCQGDHRPDCPILKDLASGCCH*
Nucleotide sequence of regulator: 399 bp .
>REG0063 NC_002516:5366257-5366655 [Pseudomonas aeruginosa PAO1]
ATGAACATCGGTGAAGCGGCGAAGAAAAGCGGACTGACGCCGAAGATGATCCGTTACTACGAGTCCATCGAGTTGCTCCG
CCCAGCCGGACGCAGCGCCAGCGGCTACCGCCACTACAACGAGAACGACCTGCATACCCTGGCGTTCATCCGCCGTTCGC
GGGACCTCGGTTTCTCCCTCGACGAAGTCGGCAAGCTGCTCACCCTCTGGCAGGATCGCCAGCGTGCCAGTGCCGACGTC
AAGGCCCTGGCGGCGCAGCACGTGCGCGAGCTGAATCGCAAGATCGAGGAGCTCAGCACCCTGCGCGACACCCTGCAGGA
CCTGGTCGAGCACTGCCAGGGCGACCACCGCCCCGACTGCCCGATCCTCAAGGACCTGGCGTCTGGCTGCTGCCACTGA
ATGAACATCGGTGAAGCGGCGAAGAAAAGCGGACTGACGCCGAAGATGATCCGTTACTACGAGTCCATCGAGTTGCTCCG
CCCAGCCGGACGCAGCGCCAGCGGCTACCGCCACTACAACGAGAACGACCTGCATACCCTGGCGTTCATCCGCCGTTCGC
GGGACCTCGGTTTCTCCCTCGACGAAGTCGGCAAGCTGCTCACCCTCTGGCAGGATCGCCAGCGTGCCAGTGCCGACGTC
AAGGCCCTGGCGGCGCAGCACGTGCGCGAGCTGAATCGCAAGATCGAGGAGCTCAGCACCCTGCGCGACACCCTGCAGGA
CCTGGTCGAGCACTGCCAGGGCGACCACCGCCCCGACTGCCCGATCCTCAAGGACCTGGCGTCTGGCTGCTGCCACTGA