Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00045  |
| Type | chaperone |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | PA1863 (ModA) |
| Description | ModA binds to molybdate in the periplasm and ModBC harbors two integral membrane permeases. |
| Reference | [1] Wang T, Du X, Ji L, et al. Pseudomonas aeruginosa T6SS-mediated molybdate transport contributes to bacterial competition during anaerobiosis. Cell Rep. 2021 Apr 13;35(2):108957. doi: 10.1016/j.celrep.2021.108957. PMID: 33852869; [2] Pederick VG, Eijkelkamp BA, Ween MP, et al. Acquisition and role of molybdate in Pseudomonas aeruginosa. Appl Environ Microbiol. 2014 Nov;80(21):6843-52. doi: 10.1128/AEM.02465-14. Epub 2014 Aug 29. PMID: 25172858; [3] Périnet S, Jeukens J, Kukavica-Ibrulj I, et al. Molybdate transporter ModABC is important for Pseudomonas aeruginosa chronic lung infection. BMC Res Notes. 2016 Jan 12;9:23. doi: 10.1186/s13104-016-1840-x. PMID: 26758577 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA1861 (ModC) |
| Coordinates (Strand) | 2020625..2021710 (-) |
| NCBI ID | 15597058 |
| RefSeq | NC_002516 |
| Uniprot ID | MODC_PSEAE |
| KEGG ID | pae:PA1861 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ABC_tran | PF00005.29 | 8.1e-32 | 19..162 |
| TOBE | PF03459.19 | 2.8e-18 | 297..360 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-361 | MDGLRLRFRRAYPGFELDIDLALPGRGVTALFGHSGSGKSTCLRCIAGLEKAAEGEVTINGETWQDSRRNLFVAPHRRALGYVFQDANLFRHLTVRRNLAFGLKRIAAAERRVELEQACALLGIEHLLERMPERLSGGEQQRVGIARALLTSPRLLLMDEPLASLDLKRKGEILPYLERLHEELDIPVLYVSHSPDEVARLADHLVLLENGKVRASGPIGETLARVDLPLALDDDAGVVIEGTVSDHDPAYGLLTLVLPGSALQMRVAHAPLAPGKRLRFKVQARDVSLNLRDDAQSSILNRLPVRVLELVDTDTAAHVLVRLDAGGNPLLARITRYSRDQLQLRPGQLLWAQIKSVAVLA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9411 | - | - |
Protein sequence: 362 a.a.
>ACP00045 NC_002516:c2021710-2020625 [Pseudomonas aeruginosa PAO1]
MDGLRLRFRRAYPGFELDIDLALPGRGVTALFGHSGSGKSTCLRCIAGLEKAAEGEVTINGETWQDSRRNLFVAPHRRAL
GYVFQDANLFRHLTVRRNLAFGLKRIAAAERRVELEQACALLGIEHLLERMPERLSGGEQQRVGIARALLTSPRLLLMDE
PLASLDLKRKGEILPYLERLHEELDIPVLYVSHSPDEVARLADHLVLLENGKVRASGPIGETLARVDLPLALDDDAGVVI
EGTVSDHDPAYGLLTLVLPGSALQMRVAHAPLAPGKRLRFKVQARDVSLNLRDDAQSSILNRLPVRVLELVDTDTAAHVL
VRLDAGGNPLLARITRYSRDQLQLRPGQLLWAQIKSVAVLA*
MDGLRLRFRRAYPGFELDIDLALPGRGVTALFGHSGSGKSTCLRCIAGLEKAAEGEVTINGETWQDSRRNLFVAPHRRAL
GYVFQDANLFRHLTVRRNLAFGLKRIAAAERRVELEQACALLGIEHLLERMPERLSGGEQQRVGIARALLTSPRLLLMDE
PLASLDLKRKGEILPYLERLHEELDIPVLYVSHSPDEVARLADHLVLLENGKVRASGPIGETLARVDLPLALDDDAGVVI
EGTVSDHDPAYGLLTLVLPGSALQMRVAHAPLAPGKRLRFKVQARDVSLNLRDDAQSSILNRLPVRVLELVDTDTAAHVL
VRLDAGGNPLLARITRYSRDQLQLRPGQLLWAQIKSVAVLA*
Nucleotide sequence: 1086 bp
>ACP00045 NC_002516:c2021710-2020625 [Pseudomonas aeruginosa PAO1]
ATGGACGGCCTGCGCCTGCGCTTCCGCCGCGCCTACCCCGGCTTCGAACTGGACATCGACCTGGCTCTTCCCGGTCGTGG
CGTGACCGCTCTGTTCGGCCATTCAGGCTCCGGCAAGAGCACTTGCCTGCGCTGTATCGCCGGCCTGGAAAAGGCCGCCG
AGGGCGAGGTGACGATCAACGGCGAGACCTGGCAGGACAGCCGCCGCAACCTCTTCGTCGCGCCCCACCGGCGTGCCCTC
GGCTACGTGTTCCAGGATGCCAACCTGTTCCGCCACCTGACGGTGCGGCGCAACCTGGCCTTCGGTCTCAAGCGCATCGC
CGCCGCCGAACGCCGCGTCGAGCTGGAACAGGCCTGCGCCCTGCTGGGCATCGAGCATCTGCTGGAACGCATGCCGGAAC
GCCTCTCCGGCGGCGAGCAGCAACGCGTCGGCATCGCCCGGGCCCTCCTCACCAGCCCGCGCCTGCTGCTGATGGACGAG
CCTCTGGCATCCCTCGACCTGAAGCGCAAGGGCGAGATCCTGCCCTATCTCGAGCGCCTGCACGAGGAACTGGACATCCC
GGTGCTCTATGTCAGCCACTCGCCAGACGAAGTGGCGCGCCTGGCCGATCACCTGGTGCTGCTGGAGAACGGCAAGGTCC
GCGCCAGCGGGCCGATCGGCGAAACCCTGGCGCGAGTCGACCTGCCGCTGGCGCTGGACGACGACGCCGGGGTGGTGATC
GAGGGCACGGTCAGCGACCACGACCCGGCCTACGGCCTGCTGACCCTGGTCCTGCCCGGCAGTGCGCTGCAAATGCGCGT
CGCCCACGCCCCGCTGGCCCCCGGCAAGCGCCTGCGCTTCAAGGTACAGGCGCGCGACGTCAGCCTGAACCTGCGCGACG
ACGCCCAGAGCAGCATCCTCAACCGCCTGCCGGTACGGGTCCTGGAATTGGTCGATACCGACACCGCGGCCCACGTCCTG
GTGCGCCTGGATGCCGGCGGCAATCCGCTGCTGGCGCGGATCACCCGTTACTCGCGGGACCAGTTGCAACTACGACCCGG
CCAGCTGTTGTGGGCGCAGATCAAGTCGGTGGCGGTGCTCGCCTGA
ATGGACGGCCTGCGCCTGCGCTTCCGCCGCGCCTACCCCGGCTTCGAACTGGACATCGACCTGGCTCTTCCCGGTCGTGG
CGTGACCGCTCTGTTCGGCCATTCAGGCTCCGGCAAGAGCACTTGCCTGCGCTGTATCGCCGGCCTGGAAAAGGCCGCCG
AGGGCGAGGTGACGATCAACGGCGAGACCTGGCAGGACAGCCGCCGCAACCTCTTCGTCGCGCCCCACCGGCGTGCCCTC
GGCTACGTGTTCCAGGATGCCAACCTGTTCCGCCACCTGACGGTGCGGCGCAACCTGGCCTTCGGTCTCAAGCGCATCGC
CGCCGCCGAACGCCGCGTCGAGCTGGAACAGGCCTGCGCCCTGCTGGGCATCGAGCATCTGCTGGAACGCATGCCGGAAC
GCCTCTCCGGCGGCGAGCAGCAACGCGTCGGCATCGCCCGGGCCCTCCTCACCAGCCCGCGCCTGCTGCTGATGGACGAG
CCTCTGGCATCCCTCGACCTGAAGCGCAAGGGCGAGATCCTGCCCTATCTCGAGCGCCTGCACGAGGAACTGGACATCCC
GGTGCTCTATGTCAGCCACTCGCCAGACGAAGTGGCGCGCCTGGCCGATCACCTGGTGCTGCTGGAGAACGGCAAGGTCC
GCGCCAGCGGGCCGATCGGCGAAACCCTGGCGCGAGTCGACCTGCCGCTGGCGCTGGACGACGACGCCGGGGTGGTGATC
GAGGGCACGGTCAGCGACCACGACCCGGCCTACGGCCTGCTGACCCTGGTCCTGCCCGGCAGTGCGCTGCAAATGCGCGT
CGCCCACGCCCCGCTGGCCCCCGGCAAGCGCCTGCGCTTCAAGGTACAGGCGCGCGACGTCAGCCTGAACCTGCGCGACG
ACGCCCAGAGCAGCATCCTCAACCGCCTGCCGGTACGGGTCCTGGAATTGGTCGATACCGACACCGCGGCCCACGTCCTG
GTGCGCCTGGATGCCGGCGGCAATCCGCTGCTGGCGCGGATCACCCGTTACTCGCGGGACCAGTTGCAACTACGACCCGG
CCAGCTGTTGTGGGCGCAGATCAAGTCGGTGGCGGTGCTCGCCTGA