SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0067
Regulator type	protein
Regulatory pathway (from literatures)	QS regulatory pathway
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Quorum sensing (map02024); Biofilm formation - Escherichia coli (map02026)
Related T6SS	T6SS00004 (Type i1)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (242 a.a.); Nucleotide sequence (726 bp)
Description	Upon RhlR and RhlI production induced by IPTG, a 3.3-fold up-regulation of the H2-T6SS promoter region was observed.
Reference	[1] Sana TG, Hachani A, Bucior I, et al. The second type VI secretion system of Pseudomonas aeruginosa strain PAO1 is regulated by quorum sensing and Fur and modulates internalization in epithelial cells. J Biol Chem. 2012 Aug 3;287(32):27095-105. doi: 10.1074/jbc.M112.376368. Epub 2012 Jun 4. PMID: 22665491

External database links

Locus tag (Gene)	PA3477 (RhlR)
Coordinates (Strand)	3889925..3890650 (-)
NCBI ID	15598673
RefSeq	NC_002516
Uniprot ID	RHLR_PSEAE
KEGG ID	pae:PA3477
KEGG pathway (determined by BlastKOALA)	map02020; map02024; map02026
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS transcript	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Autoind_bind	PF03472.17	8.9e-36	26..164
GerE	PF00196.21	2.1e-19	181..235

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-241	MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGLI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9845	-	-

Protein sequence of regulator: 242 a.a. .

                                    >REG0067 NC_002516:c3890650-3889925 [Pseudomonas aeruginosa PAO1]
MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAV
DPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIE
LLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGL
I*

                                 

Nucleotide sequence of regulator: 726 bp .

                                    >REG0067 NC_002516:c3890650-3889925 [Pseudomonas aeruginosa PAO1]
ATGAGGAATGACGGAGGCTTTTTGCTGTGGTGGGACGGTTTGCGTAGCGAGATGCAGCCGATCCACGACAGCCAGGGCGT
GTTCGCCGTCCTGGAAAAGGAAGTGCGGCGCCTGGGCTTCGATTACTACGCCTATGGCGTGCGCCACACGATTCCCTTCA
CCCGGCCGAAGACCGAGGTCCATGGCACCTATCCCAAGGCCTGGCTGGAGCGATACCAGATGCAGAACTACGGGGCCGTG
GATCCGGCGATCCTCAACGGCCTGCGCTCCTCGGAAATGGTGGTCTGGAGCGACAGCCTGTTCGACCAGAGCCGGATGCT
CTGGAACGAGGCTCGCGATTGGGGCCTCTGTGTCGGCGCGACCTTGCCGATCCGCGCGCCGAACAATTTGCTCAGCGTGC
TTTCCGTGGCGCGCGACCAGCAGAACATCTCCAGCTTCGAGCGCGAGGAAATCCGCCTGCGGCTGCGTTGCATGATCGAG
TTGCTGACCCAGAAGCTGACCGACCTGGAGCATCCGATGCTGATGTCCAACCCGGTCTGCCTGAGCCATCGCGAACGCGA
GATCCTGCAATGGACCGCCGACGGCAAGAGTTCCGGGGAAATCGCCATCATCCTGAGCATCTCCGAGAGCACGGTGAACT
TCCACCACAAGAACATCCAGAAGAAGTTCGACGCGCCGAACAAGACGCTGGCTGCCGCCTACGCCGCGGCGCTGGGTCTC
ATCTGA

                                