Detailed information of accessory protein


Summary

Accessory protein ID ACP00004 experimental
Type structure affiliated protein
Related T6SS (Type) T6SS00003 (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Related structure protein PA0083 (HisB1/TssB1)
Related effector -
Description Coomassie staining and immunoblotting shows HsiE1 can be co-purified with HsiB1 that demonstrating that HsiB1 and HsiE1 form a stable complex
Reference [1] Lossi NS, Manoli E, Simpson P, et al. The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel subcomplex in the bacterial type VI secretion system. Mol Microbiol. 2012 Oct;86(2):437-56. doi: 10.1111/j.1365-2958.2012.08204.x. Epub 2012 Aug 29. PMID: 22906320
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) PA0086 (tagJ)
Coordinates (Strand) 104006..104851 (+)
NCBI ID 15595284
RefSeq NC_002516
Uniprot ID TAGJ_PSEAE
KEGG ID pae:PA0086
PDB ID 4UQZ, 4UQX, 4UQY
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpE PF07024.15 1.5e-43 159..274
TPR_19 PF14559.8 2.8e-06 26..82
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-281 MADPSFASGRLGSRLQGSIAMIAEELLRAGRLDDALKALQEQVRSQPSNATLRIFLFQLLAVMGQWARAQNQLKVVGELDASALPMVQTYSTAIDCEALRREVFAGRLTPVILGQPAEWIAPLLQALSLDAEGHGEAAQALREQAFDAAPAVPGRIGEAPFAWLADADTRLGPVLEVIVNGRYAWLPMSNLRSLKVEAPSDLRDLVWLPAELTLANGGATVALLPARYAETVEHGDDAARLGRKTEWLDSGLPVGQRLFVTDAGETALFDLRELDFEPTDA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9704 - -


  Protein sequence: 282 a.a.    

>ACP00004 NC_002516:104006-104851 [Pseudomonas aeruginosa PAO1]
MADPSFASGRLGSRLQGSIAMIAEELLRAGRLDDALKALQEQVRSQPSNATLRIFLFQLLAVMGQWARAQNQLKVVGELD
ASALPMVQTYSTAIDCEALRREVFAGRLTPVILGQPAEWIAPLLQALSLDAEGHGEAAQALREQAFDAAPAVPGRIGEAP
FAWLADADTRLGPVLEVIVNGRYAWLPMSNLRSLKVEAPSDLRDLVWLPAELTLANGGATVALLPARYAETVEHGDDAAR
LGRKTEWLDSGLPVGQRLFVTDAGETALFDLRELDFEPTDA*

  Nucleotide sequence: 846 bp    

>ACP00004 NC_002516:104006-104851 [Pseudomonas aeruginosa PAO1]
ATGGCCGACCCTTCATTCGCATCCGGGCGCCTGGGCTCCCGGCTACAGGGAAGTATCGCGATGATCGCCGAAGAACTGTT
GCGCGCCGGGCGCCTGGACGATGCCTTGAAAGCCTTGCAGGAACAGGTGCGCAGCCAGCCGTCGAACGCTACCCTGCGGA
TCTTCCTGTTCCAGCTGCTGGCCGTGATGGGCCAGTGGGCGCGGGCGCAGAACCAGCTCAAGGTGGTCGGCGAACTGGAT
GCCTCGGCGCTGCCGATGGTACAGACCTACTCCACCGCCATCGACTGCGAGGCGCTGCGCCGCGAGGTGTTCGCCGGGCG
CCTGACGCCGGTGATCCTCGGCCAGCCGGCGGAATGGATCGCGCCGCTGTTGCAGGCCCTGAGCCTGGACGCCGAAGGCC
ATGGCGAGGCCGCCCAGGCCCTGCGCGAGCAGGCCTTCGACGCCGCGCCGGCGGTGCCCGGTCGGATCGGCGAAGCGCCG
TTCGCCTGGCTGGCGGATGCCGACACGCGCCTCGGCCCGGTGCTCGAGGTAATCGTCAACGGTCGCTATGCCTGGTTGCC
GATGAGCAACCTGCGCAGCCTCAAGGTCGAGGCGCCGAGCGACCTGCGCGATCTGGTCTGGCTGCCGGCCGAACTGACCC
TGGCCAACGGTGGCGCCACCGTCGCGCTGCTGCCGGCGCGCTACGCGGAAACCGTCGAGCACGGCGACGACGCCGCGCGC
CTGGGACGCAAGACCGAATGGCTGGACAGCGGCCTGCCGGTCGGCCAGCGGCTGTTCGTCACCGATGCCGGCGAGACCGC
GCTGTTCGACCTGCGTGAACTGGACTTCGAGCCGACGGACGCCTGA