Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00274  |
| Cognate effector | EFF01037 |
| Related T6SS (Type) | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (146 a.a.); Nucleotide sequence (438 bp) |
| Description | Tsi4 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA2775 (Tsi4) |
| Coordinates (Strand) | 3132821..3133258 (-) |
| NCBI ID | 15597971 |
| RefSeq | NC_002516 |
| Uniprot ID | TSI4_PSEAE |
| KEGG ID | pae:PA2775 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF3592 | PF12158.10 | 1.1e-15 | 9..133 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-8 | MTTSSGSR |
| Transmembrane helix | 9-31 | IGGLLHASLFSLLGLGLLLAGGF |
| Outside | 32-106 | KTVERYHFLRTAQEAQGTVSALNAGGSHPQIDFTSVSGERISYPQGGFIFGYQVGEPVRVLYEAGRPAASAIVDD |
| Transmembrane helix | 107-129 | AGALWGTGAFLCGFGALFGIVGF |
| Inside | 130-145 | RGLLGLRSSQPTSKGH |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7578 | - | - |
Protein sequence: 146 a.a. .
>IMU00274 NC_002516:c3133258-3132821 [Pseudomonas aeruginosa PAO1]
MTTSSGSRIGGLLHASLFSLLGLGLLLAGGFKTVERYHFLRTAQEAQGTVSALNAGGSHPQIDFTSVSGERISYPQGGFI
FGYQVGEPVRVLYEAGRPAASAIVDDAGALWGTGAFLCGFGALFGIVGFRGLLGLRSSQPTSKGH*
MTTSSGSRIGGLLHASLFSLLGLGLLLAGGFKTVERYHFLRTAQEAQGTVSALNAGGSHPQIDFTSVSGERISYPQGGFI
FGYQVGEPVRVLYEAGRPAASAIVDDAGALWGTGAFLCGFGALFGIVGFRGLLGLRSSQPTSKGH*
Nucleotide sequence: 438 bp .
>IMU00274 NC_002516:c3133258-3132821 [Pseudomonas aeruginosa PAO1]
ATGACGACCTCTTCCGGTTCGCGTATCGGCGGACTTCTTCACGCTTCCCTTTTTTCCCTGCTCGGACTCGGTCTCCTGCT
CGCCGGCGGTTTCAAGACCGTCGAGCGGTACCATTTCCTGCGTACTGCGCAGGAAGCCCAGGGGACGGTCAGCGCCTTGA
ATGCCGGCGGTTCGCATCCGCAGATCGATTTCACCAGCGTTTCCGGCGAGCGGATTTCCTATCCCCAGGGCGGCTTCATC
TTCGGCTACCAGGTGGGCGAGCCGGTTCGCGTCCTCTACGAGGCTGGGCGGCCGGCGGCCAGCGCGATAGTCGACGATGC
CGGCGCGCTCTGGGGAACCGGCGCTTTCCTCTGCGGCTTCGGCGCACTCTTCGGTATTGTCGGGTTCAGGGGATTGCTGG
GCCTGCGTTCTTCCCAACCGACCTCAAAAGGACATTGA
ATGACGACCTCTTCCGGTTCGCGTATCGGCGGACTTCTTCACGCTTCCCTTTTTTCCCTGCTCGGACTCGGTCTCCTGCT
CGCCGGCGGTTTCAAGACCGTCGAGCGGTACCATTTCCTGCGTACTGCGCAGGAAGCCCAGGGGACGGTCAGCGCCTTGA
ATGCCGGCGGTTCGCATCCGCAGATCGATTTCACCAGCGTTTCCGGCGAGCGGATTTCCTATCCCCAGGGCGGCTTCATC
TTCGGCTACCAGGTGGGCGAGCCGGTTCGCGTCCTCTACGAGGCTGGGCGGCCGGCGGCCAGCGCGATAGTCGACGATGC
CGGCGCGCTCTGGGGAACCGGCGCTTTCCTCTGCGGCTTCGGCGCACTCTTCGGTATTGTCGGGTTCAGGGGATTGCTGG
GCCTGCGTTCTTCCCAACCGACCTCAAAAGGACATTGA