SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00333
Cognate effector	EFF01318
Related T6SS (Type)	T6SS00003 (Type i3)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (307 a.a.); Nucleotide sequence (921 bp)
Description	-
Reference	[1] Pissaridou P, Allsopp LP, Wettstadt S, et al. The Pseudomonas aeruginosa T6SS-VgrG1b spike is topped by a PAAR protein eliciting DNA damage to bacterial competitors. Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12519-12524. doi: 10.1073/pnas.1814181115. Epub 2018 Nov 19. PMID: 30455305

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA0100 (Tsi7)
Coordinates (Strand)	121346..122266 (+)
NCBI ID	15595299
RefSeq	NC_002516
Uniprot ID	Q9I731_PSEAE
KEGG ID	pae:PA0101
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-306	MLKKLSPIFSNITGVVRYQDLAYVASVSDEIQEQNIAHSYVTEWDCGTWCVAGEDDDMLPWEIVSATVVHEPVEQALFLGARGQVFCMGSGDIHEEQLPDGDDAIGGRGNMRGVACIDGVAYACGMDRQVYRRFDENDWRAIDTGARPPAGSEAVVGFEAIGGFGAREIYAVGWDGEIWQYDGKRWQPRESPTNLILTAICCAEDGSVYACGQAGTLLRGRNDHWEIIAQDDVDEDLWSLAWFDGALYVSSATAVYTLVGGHLKEVDFGDEQPQRCFHLSAADGVLWSIAAKDIFSFDGQQWTRID

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9517	-	-

Protein sequence: 307 a.a. .

                                    >IMU00333 NC_002516:121346-122266 [Pseudomonas aeruginosa PAO1]
MLKKLSPIFSNITGVVRYQDLAYVASVSDEIQEQNIAHSYVTEWDCGTWCVAGEDDDMLPWEIVSATVVHEPVEQALFLG
ARGQVFCMGSGDIHEEQLPDGDDAIGGRGNMRGVACIDGVAYACGMDRQVYRRFDENDWRAIDTGARPPAGSEAVVGFEA
IGGFGAREIYAVGWDGEIWQYDGKRWQPRESPTNLILTAICCAEDGSVYACGQAGTLLRGRNDHWEIIAQDDVDEDLWSL
AWFDGALYVSSATAVYTLVGGHLKEVDFGDEQPQRCFHLSAADGVLWSIAAKDIFSFDGQQWTRID*

                                

Nucleotide sequence: 921 bp .

                                    >IMU00333 NC_002516:121346-122266 [Pseudomonas aeruginosa PAO1]
ATGTTGAAGAAACTCTCGCCGATTTTCTCGAACATCACCGGCGTCGTCCGCTATCAGGACCTGGCGTACGTGGCCTCGGT
ATCCGACGAGATCCAGGAGCAGAACATAGCGCACAGCTATGTCACGGAGTGGGACTGCGGAACCTGGTGTGTTGCTGGCG
AGGATGACGACATGCTGCCCTGGGAGATCGTGTCGGCCACGGTAGTGCACGAGCCGGTGGAACAAGCATTGTTTCTGGGC
GCTCGGGGCCAGGTATTCTGCATGGGCTCCGGCGACATCCATGAAGAGCAGTTGCCCGACGGCGACGATGCCATCGGTGG
CCGGGGCAATATGCGCGGCGTTGCCTGTATCGACGGGGTGGCCTATGCCTGCGGGATGGATCGACAGGTCTACCGGCGCT
TCGACGAGAACGACTGGCGTGCCATCGACACCGGCGCTCGGCCTCCGGCAGGCAGCGAAGCGGTGGTCGGATTCGAGGCC
ATCGGCGGCTTCGGTGCCAGGGAAATCTATGCCGTCGGCTGGGACGGGGAGATCTGGCAGTACGACGGCAAGCGCTGGCA
GCCCCGGGAGAGCCCGACGAACTTGATCCTGACGGCGATCTGCTGCGCGGAAGATGGCTCGGTTTACGCCTGTGGACAGG
CGGGAACGCTGCTGCGTGGACGCAACGACCACTGGGAGATCATCGCCCAGGACGATGTCGATGAAGACTTGTGGAGCCTC
GCGTGGTTCGACGGCGCACTCTACGTGTCGAGCGCAACGGCGGTCTATACGCTGGTCGGTGGCCATCTCAAGGAGGTGGA
CTTCGGTGATGAGCAACCCCAACGCTGCTTCCACCTTAGCGCTGCCGACGGCGTGTTGTGGAGCATCGCCGCGAAAGACA
TCTTCAGCTTCGACGGCCAGCAATGGACGCGCATAGATTGA

                                