Detailed information of regulator
Summary
| Regulator ID | REG0047  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (400 a.a.); Nucleotide sequence (1200 bp) |
| Description | Individual isogenic PAO1 mutants (ΔtagT, ΔtagS and ΔtagQ) were impaired in Hcp1 export, co-production of TagS and TagT resulted in formation of a stable protein complex. TagT, TagS and TagQ act upstream of PpkA in the TPP. |
| Reference |
External database links
| Locus tag (Gene) | PA0072 (TagS) |
| Coordinates (Strand) | 85085..86284 (-) |
| NCBI ID | 15595270 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I760_PSEAE |
| KEGG ID | pae:PA0072 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| PA0073 (TagT) | ↓ | 15595271 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MacB_PCD | PF12704.9 | 4.2e-06 | 21..195 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-19 | MRAGLLLSLAWADYRGEAR |
| Transmembrane helix | 20-39 | LSLCAIFALAAVITPLLVLF |
| Outside | 40-271 | GLKYGLVSTLTERLERAPSVREIIPVGGARYRAEDIAALAARADVAFAVPRTRQIAATADLSGGGETALSVEMIPSAAGDPLLAGLPQPDRPTRVVLSHGAAEKLGAQPGERIVARIGRRMDGQAQSQRLELEVLAVLPQERFARDALFAPLALLEAAEDYRDGRAVAAYGWPGKAGETPRARIYPGFRLYARDLDAVEGLRRHFVASGVEVATQAEAIAQVRSLSRNLGLV |
| Transmembrane helix | 272-294 | FWIVAALAIGGAFAAIAASSLAA |
| Inside | 295-300 | VERKRR |
| Transmembrane helix | 301-323 | ALAVLRLLGFPTAALVGFVMLLA |
| Outside | 324-326 | LFS |
| Transmembrane helix | 327-349 | AVFGLFLAGLLYAATAGALNHLF |
| Inside | 350-360 | DNQSGEFVCRL |
| Transmembrane helix | 361-383 | LPSHYLTALLATLLCSVLAAASG |
| Outside | 384-399 | GWRAARIEAAEGLRDV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9725 | - | - |
Protein sequence of regulator: 400 a.a. .
>REG0047 NC_002516:c86284-85085 [Pseudomonas aeruginosa PAO1]
MRAGLLLSLAWADYRGEARLSLCAIFALAAVITPLLVLFGLKYGLVSTLTERLERAPSVREIIPVGGARYRAEDIAALAA
RADVAFAVPRTRQIAATADLSGGGETALSVEMIPSAAGDPLLAGLPQPDRPTRVVLSHGAAEKLGAQPGERIVARIGRRM
DGQAQSQRLELEVLAVLPQERFARDALFAPLALLEAAEDYRDGRAVAAYGWPGKAGETPRARIYPGFRLYARDLDAVEGL
RRHFVASGVEVATQAEAIAQVRSLSRNLGLVFWIVAALAIGGAFAAIAASSLAAVERKRRALAVLRLLGFPTAALVGFVM
LLALFSAVFGLFLAGLLYAATAGALNHLFDNQSGEFVCRLLPSHYLTALLATLLCSVLAAASGGWRAARIEAAEGLRDV*
MRAGLLLSLAWADYRGEARLSLCAIFALAAVITPLLVLFGLKYGLVSTLTERLERAPSVREIIPVGGARYRAEDIAALAA
RADVAFAVPRTRQIAATADLSGGGETALSVEMIPSAAGDPLLAGLPQPDRPTRVVLSHGAAEKLGAQPGERIVARIGRRM
DGQAQSQRLELEVLAVLPQERFARDALFAPLALLEAAEDYRDGRAVAAYGWPGKAGETPRARIYPGFRLYARDLDAVEGL
RRHFVASGVEVATQAEAIAQVRSLSRNLGLVFWIVAALAIGGAFAAIAASSLAAVERKRRALAVLRLLGFPTAALVGFVM
LLALFSAVFGLFLAGLLYAATAGALNHLFDNQSGEFVCRLLPSHYLTALLATLLCSVLAAASGGWRAARIEAAEGLRDV*
Nucleotide sequence of regulator: 1200 bp .
>REG0047 NC_002516:c86284-85085 [Pseudomonas aeruginosa PAO1]
ATGCGCGCCGGCCTGCTGCTCTCGCTGGCCTGGGCCGACTACCGCGGCGAGGCGCGCCTGTCGCTCTGCGCGATCTTCGC
CCTGGCGGCGGTGATCACCCCGCTGCTGGTGCTGTTCGGCCTCAAGTACGGCCTGGTCAGCACCCTCACCGAGCGGCTGG
AGCGCGCGCCTTCGGTGCGCGAGATCATTCCGGTCGGCGGCGCCCGCTACCGTGCCGAGGACATCGCCGCCCTGGCCGCA
CGCGCGGACGTCGCCTTCGCCGTGCCGCGCACCCGGCAGATCGCCGCCACCGCCGACCTCAGCGGCGGCGGCGAGACCGC
CCTGAGCGTCGAGATGATTCCCAGCGCCGCCGGCGACCCGCTGCTGGCCGGCCTGCCGCAACCCGACCGGCCGACCCGCG
TGGTGCTCAGCCACGGCGCCGCGGAAAAACTCGGCGCCCAGCCTGGCGAGCGCATCGTCGCGCGCATCGGCCGGCGCATG
GATGGCCAGGCACAGAGCCAGCGCCTGGAGCTGGAAGTGCTCGCGGTGCTGCCGCAGGAGCGTTTCGCCCGCGACGCGCT
GTTCGCCCCGCTGGCCCTGCTGGAAGCCGCCGAGGACTATCGCGACGGTCGCGCGGTGGCCGCCTACGGCTGGCCGGGAA
AGGCCGGCGAGACCCCGCGCGCACGCATCTACCCGGGCTTCCGCCTGTACGCCCGAGACCTCGATGCGGTGGAAGGGCTG
CGCCGGCACTTCGTCGCGAGTGGCGTGGAAGTCGCCACCCAGGCCGAGGCCATCGCCCAGGTGCGCAGCCTCAGCCGCAA
CCTCGGCCTGGTGTTCTGGATCGTCGCCGCGCTGGCCATCGGCGGTGCCTTCGCCGCCATCGCCGCCAGCAGCCTGGCCG
CCGTCGAACGCAAGCGCCGGGCCCTGGCGGTGCTACGTCTGCTGGGCTTCCCGACCGCCGCGCTGGTCGGCTTCGTCATG
CTCCTGGCGCTGTTCAGCGCCGTCTTCGGCCTGTTCCTCGCCGGGCTGCTGTACGCCGCCACGGCCGGCGCGCTGAACCA
CCTGTTCGACAACCAGAGCGGCGAGTTCGTCTGCCGCCTGCTGCCCAGCCACTACCTGACGGCGCTGCTCGCCACGCTGC
TGTGCAGCGTCCTGGCCGCCGCCAGCGGCGGCTGGCGCGCGGCGCGGATCGAAGCCGCAGAGGGATTACGCGATGTTTGA
ATGCGCGCCGGCCTGCTGCTCTCGCTGGCCTGGGCCGACTACCGCGGCGAGGCGCGCCTGTCGCTCTGCGCGATCTTCGC
CCTGGCGGCGGTGATCACCCCGCTGCTGGTGCTGTTCGGCCTCAAGTACGGCCTGGTCAGCACCCTCACCGAGCGGCTGG
AGCGCGCGCCTTCGGTGCGCGAGATCATTCCGGTCGGCGGCGCCCGCTACCGTGCCGAGGACATCGCCGCCCTGGCCGCA
CGCGCGGACGTCGCCTTCGCCGTGCCGCGCACCCGGCAGATCGCCGCCACCGCCGACCTCAGCGGCGGCGGCGAGACCGC
CCTGAGCGTCGAGATGATTCCCAGCGCCGCCGGCGACCCGCTGCTGGCCGGCCTGCCGCAACCCGACCGGCCGACCCGCG
TGGTGCTCAGCCACGGCGCCGCGGAAAAACTCGGCGCCCAGCCTGGCGAGCGCATCGTCGCGCGCATCGGCCGGCGCATG
GATGGCCAGGCACAGAGCCAGCGCCTGGAGCTGGAAGTGCTCGCGGTGCTGCCGCAGGAGCGTTTCGCCCGCGACGCGCT
GTTCGCCCCGCTGGCCCTGCTGGAAGCCGCCGAGGACTATCGCGACGGTCGCGCGGTGGCCGCCTACGGCTGGCCGGGAA
AGGCCGGCGAGACCCCGCGCGCACGCATCTACCCGGGCTTCCGCCTGTACGCCCGAGACCTCGATGCGGTGGAAGGGCTG
CGCCGGCACTTCGTCGCGAGTGGCGTGGAAGTCGCCACCCAGGCCGAGGCCATCGCCCAGGTGCGCAGCCTCAGCCGCAA
CCTCGGCCTGGTGTTCTGGATCGTCGCCGCGCTGGCCATCGGCGGTGCCTTCGCCGCCATCGCCGCCAGCAGCCTGGCCG
CCGTCGAACGCAAGCGCCGGGCCCTGGCGGTGCTACGTCTGCTGGGCTTCCCGACCGCCGCGCTGGTCGGCTTCGTCATG
CTCCTGGCGCTGTTCAGCGCCGTCTTCGGCCTGTTCCTCGCCGGGCTGCTGTACGCCGCCACGGCCGGCGCGCTGAACCA
CCTGTTCGACAACCAGAGCGGCGAGTTCGTCTGCCGCCTGCTGCCCAGCCACTACCTGACGGCGCTGCTCGCCACGCTGC
TGTGCAGCGTCCTGGCCGCCGCCAGCGGCGGCTGGCGCGCGGCGCGGATCGAAGCCGCAGAGGGATTACGCGATGTTTGA