Detailed information of regulator


Summary

Regulator ID REG0057 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) Bacterial secretion system (map03070); Biofilm formation - Pseudomonas aeruginosa (map02025)
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (498 a.a.); Nucleotide sequence (1494 bp)
Description p-Fha1 is critical to trigger H-T6SS.; Western blot analysis of TAP eluents and FM results shows ClpV1, and possibly other key H1-T6S components, are in complex with Fha1. Phosphorylation of Fha1 by PpkA recruits this complex to putative membrane-bound and periplasmic components of the apparatus.
Reference
External database links

Locus tag (Gene) PA0081 (Fha-1/TagH)
Coordinates (Strand) 98263..99756 (-)
NCBI ID 15595279
RefSeq NC_002516
Uniprot ID Q9I751_PSEAE
KEGG ID pae:PA0081
KEGG pathway (determined by BlastKOALA) map03070; map02025
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Unknown ↓ -
clpV ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
FHA PF00498.28 1.2e-14 29..97
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-497 MPLRLTITSYHKLTPGQCSEKVLDQGQLTIGRGPDNDWVLPDPERLVSSRHCTILNRDGVYYLTDTSTNGVLLVNAGHRLRRGNSEPLQDGETVRLGEYDILVQLGHDIALPGSGNPQTDPFTSFDALMSRQAAGSAPAFAEPAPTPHPAVTAHFQGGSPLDTKPDLFDFLTPPPPGAAPRPDHVPAEQHDFRPPEPVIPPPPATTPAPPPAGGAPLIPADWDPFAELLGNTPAPSATPVAQPLPTAEPTPLAMPFADPGITQQPQPQPQPQPQPQPQPQPQPQPASVAAPTPPASAAASAGGDLLQAFLRGAGMTQLKVDPAGAEAQMEAIGRSYRGLVEGLVDVLRARASLKGEFRMAQTMIQPVQNNPLKFAPNVDEAMLLLLRRDNQAFMAPDRAVADSFEDLKAHQLAVMAGVQAAIRHLLARFEPAALEARFGKPAGLSGLLPGARQAQNWDSFTELYAKILREAEDDFQELFGREFSRAYEEHSARLRRS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9466 - -


  Protein sequence of regulator: 498 a.a.    .

>REG0057 NC_002516:c99756-98263 [Pseudomonas aeruginosa PAO1]
MPLRLTITSYHKLTPGQCSEKVLDQGQLTIGRGPDNDWVLPDPERLVSSRHCTILNRDGVYYLTDTSTNGVLLVNAGHRL
RRGNSEPLQDGETVRLGEYDILVQLGHDIALPGSGNPQTDPFTSFDALMSRQAAGSAPAFAEPAPTPHPAVTAHFQGGSP
LDTKPDLFDFLTPPPPGAAPRPDHVPAEQHDFRPPEPVIPPPPATTPAPPPAGGAPLIPADWDPFAELLGNTPAPSATPV
AQPLPTAEPTPLAMPFADPGITQQPQPQPQPQPQPQPQPQPQPQPASVAAPTPPASAAASAGGDLLQAFLRGAGMTQLKV
DPAGAEAQMEAIGRSYRGLVEGLVDVLRARASLKGEFRMAQTMIQPVQNNPLKFAPNVDEAMLLLLRRDNQAFMAPDRAV
ADSFEDLKAHQLAVMAGVQAAIRHLLARFEPAALEARFGKPAGLSGLLPGARQAQNWDSFTELYAKILREAEDDFQELFG
REFSRAYEEHSARLRRS*

  Nucleotide sequence of regulator: 1494 bp    .

>REG0057 NC_002516:c99756-98263 [Pseudomonas aeruginosa PAO1]
ATGCCGCTGCGATTGACCATCACCAGCTACCACAAGCTGACCCCCGGTCAGTGTTCGGAAAAGGTACTGGACCAGGGACA
GCTGACCATCGGTCGGGGCCCGGACAACGACTGGGTCCTGCCGGACCCGGAGCGCCTGGTCTCCAGCCGCCACTGCACCA
TCCTCAACCGCGACGGGGTGTACTACCTCACCGACACCAGCACCAACGGCGTGCTGCTGGTCAATGCCGGGCACCGCCTG
CGCCGCGGCAACAGCGAGCCGCTGCAGGACGGCGAGACCGTCCGCCTCGGCGAATACGACATCCTCGTCCAGCTCGGCCA
TGACATCGCCCTGCCCGGCAGCGGCAATCCGCAGACCGATCCGTTCACCAGCTTCGACGCCCTGATGAGCCGGCAGGCCG
CCGGATCGGCGCCTGCCTTCGCCGAACCGGCGCCCACCCCGCACCCCGCGGTGACGGCGCATTTCCAGGGCGGCTCGCCG
CTGGACACCAAGCCCGACCTGTTCGACTTCCTCACCCCGCCGCCGCCCGGCGCGGCGCCGCGTCCGGACCATGTACCGGC
CGAACAGCACGACTTCCGCCCGCCGGAACCGGTGATCCCGCCGCCCCCCGCGACCACCCCGGCGCCACCGCCCGCTGGCG
GCGCACCGCTGATTCCGGCCGACTGGGACCCGTTCGCCGAACTGCTCGGCAACACGCCCGCGCCAAGCGCCACGCCCGTC
GCCCAGCCGCTGCCGACGGCCGAACCGACGCCGCTGGCGATGCCCTTCGCCGACCCAGGGATCACCCAACAGCCACAGCC
ACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCAGCGAGCGTGGCGGCGCCGACGCCGC
CGGCAAGCGCCGCCGCGTCGGCCGGCGGCGACCTGCTGCAAGCGTTCCTGCGCGGCGCCGGCATGACCCAGTTGAAGGTC
GACCCGGCCGGCGCCGAAGCGCAGATGGAAGCCATCGGCCGCAGCTATCGCGGCCTGGTGGAAGGACTGGTGGACGTGCT
GCGCGCGCGGGCCAGCCTGAAGGGCGAGTTCCGCATGGCGCAGACCATGATCCAGCCGGTGCAGAACAACCCGCTGAAGT
TCGCGCCGAACGTCGACGAGGCCATGCTGCTCCTGCTGCGCCGCGACAACCAGGCGTTCATGGCGCCGGATCGCGCGGTG
GCCGACAGCTTCGAAGACCTCAAGGCGCACCAGCTGGCGGTGATGGCCGGCGTGCAGGCCGCCATCCGCCACCTGCTGGC
GCGCTTCGAACCGGCCGCGCTGGAAGCCCGCTTCGGCAAGCCGGCCGGGCTTTCCGGCCTGCTGCCGGGGGCCCGCCAGG
CACAGAACTGGGACAGTTTCACCGAGCTGTACGCGAAGATCCTGCGCGAGGCGGAGGACGACTTCCAGGAGCTTTTCGGC
CGCGAATTCAGCCGCGCCTACGAGGAACACAGCGCTCGACTACGGCGTTCCTGA