Detailed information of regulator


Summary

Regulator ID REG0258 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus strain EPJS
Replicon chromosome
Sequence Protein sequence (717 a.a.); Nucleotide sequence (2151 bp)
Description PppA could be directly or indirectly phosphorylated by PpkA2 at T253 and Phosphorylation of PppA modulates the expression of Hcp2 by regulating LuxR; T6SS2 secretion and killing is controlled by PpkA2 and PpkA2 autophosphorylation at residue Thr 59 as well as PpkA2 phosphorylated DotU2 at two threonine residues, T4 and T28 is required for T6SS2 function
Reference
External database links

Locus tag (Gene) VEPGS_0606 (PpkA2)
Coordinates (Strand) 3227..5377 (-)
NCBI ID 225382172
GenBank FJ790777
Uniprot ID C0LZP6_VIBAL
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
LuxR ↓ -
VEPGS_0610 (DotU2) ↓ 225382176
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Pkinase PF00069.27 3.4e-39 93..336
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-716 MEDSNKNNSINNEESGDKTKIVKTQKRDNAIKSTKLTNNETSVKNKNETNTKLSQKENTPLVNKKKNNKDDSLNNNKDVINESLIGTTVKNRYEIESVIGHGGLCDVYLAKDKILESSGSETPYVALKILQKEFASQPETARMLIREAQQTQRLSHPNIIRVFDFGVDGEIYFLVMEYINGETLESLIQRSRPHGLKFNATLSILNQVLDALSYAHSLGVVHADLKPANVILTSEGDVKLLDFGVSKTHQLKQDQYAAKRKSENVETLGYTPNYASLNLLSGKEPNLSDDMFALFCIAYELLSCKHPYARTPVNEALKKKIKVSKPTNMPASKWPIFSSVFSTGIIPYELTPEQLKRKLNMRYWPIASGAAASILLVSGIVFGYQQQQETIATLETVISKQNDQIAGQEWQLDTPAEKSLALIDSEKSLHPVVKAGLLRLHKTYILSEFESEIDQVLNSDANNYPNYDEIESILDKAKTYYPDSHKIEVLALDIQSSKHSTLLSIARQINSQLEKSRYSKEDGSKSIYELKDELNQIHQGYPFTPSSLSSDVFGQHLTEALKKKDAAALVALIKVGNTFFSNVEEHQENLQISNAMKDAVLEMKLYETALDSNSPLPFPEDAARILFQEEFENFYSRLEKARTTTHLDNLVKDMDLFSKNFPIGFQDVNDLRFKTADKYLRFSDILLNKRRTSSARRAMKKANELMKQIEQDSKQS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9520 - -


  Protein sequence of regulator: 717 a.a.    .

>REG0258 FJ790777:c5377-3227 [Vibrio alginolyticus]
MEDSNKNNSINNEESGDKTKIVKTQKRDNAIKSTKLTNNETSVKNKNETNTKLSQKENTPLVNKKKNNKDDSLNNNKDVI
NESLIGTTVKNRYEIESVIGHGGLCDVYLAKDKILESSGSETPYVALKILQKEFASQPETARMLIREAQQTQRLSHPNII
RVFDFGVDGEIYFLVMEYINGETLESLIQRSRPHGLKFNATLSILNQVLDALSYAHSLGVVHADLKPANVILTSEGDVKL
LDFGVSKTHQLKQDQYAAKRKSENVETLGYTPNYASLNLLSGKEPNLSDDMFALFCIAYELLSCKHPYARTPVNEALKKK
IKVSKPTNMPASKWPIFSSVFSTGIIPYELTPEQLKRKLNMRYWPIASGAAASILLVSGIVFGYQQQQETIATLETVISK
QNDQIAGQEWQLDTPAEKSLALIDSEKSLHPVVKAGLLRLHKTYILSEFESEIDQVLNSDANNYPNYDEIESILDKAKTY
YPDSHKIEVLALDIQSSKHSTLLSIARQINSQLEKSRYSKEDGSKSIYELKDELNQIHQGYPFTPSSLSSDVFGQHLTEA
LKKKDAAALVALIKVGNTFFSNVEEHQENLQISNAMKDAVLEMKLYETALDSNSPLPFPEDAARILFQEEFENFYSRLEK
ARTTTHLDNLVKDMDLFSKNFPIGFQDVNDLRFKTADKYLRFSDILLNKRRTSSARRAMKKANELMKQIEQDSKQS*

  Nucleotide sequence of regulator: 2151 bp    .

>REG0258 FJ790777:c5377-3227 [Vibrio alginolyticus]
ATGGAAGACAGTAATAAAAACAATTCTATAAATAACGAAGAAAGCGGAGATAAAACTAAAATTGTAAAGACTCAAAAACG
AGACAATGCAATTAAATCAACCAAACTCACTAATAATGAAACCTCCGTTAAAAATAAAAATGAAACAAATACAAAACTGT
CTCAAAAAGAAAACACTCCGTTAGTTAATAAGAAAAAAAACAATAAAGATGACTCATTAAATAATAACAAAGATGTTATT
AACGAAAGCTTAATAGGAACGACCGTTAAAAATCGGTATGAAATTGAGTCGGTTATTGGGCATGGTGGGCTTTGCGATGT
TTACTTAGCTAAAGATAAGATTCTTGAATCTTCAGGTTCAGAAACACCCTACGTTGCGTTAAAAATATTACAGAAAGAGT
TTGCTTCTCAACCAGAGACTGCACGCATGTTAATACGTGAAGCTCAACAAACTCAAAGGTTAAGCCACCCTAATATCATT
AGGGTTTTTGATTTTGGTGTTGATGGCGAAATATACTTTTTAGTTATGGAGTATATTAACGGAGAAACATTAGAGAGTTT
AATACAGCGTTCTAGGCCCCATGGGCTGAAGTTTAATGCAACTTTATCAATCCTAAATCAGGTACTCGATGCACTCTCAT
ATGCTCACTCTCTTGGTGTAGTGCACGCCGACCTAAAGCCCGCTAATGTGATTTTAACGTCTGAGGGTGACGTTAAGCTG
CTTGATTTTGGTGTATCTAAAACTCATCAATTAAAGCAGGATCAATATGCGGCTAAACGTAAATCCGAAAATGTTGAAAC
GCTTGGTTATACTCCCAACTATGCATCACTCAACCTGTTATCCGGTAAGGAACCGAACTTAAGCGACGATATGTTTGCTC
TATTTTGTATTGCTTACGAGTTATTGAGTTGCAAACACCCTTACGCAAGAACTCCAGTTAATGAAGCTCTCAAAAAGAAG
ATCAAGGTCTCTAAACCAACTAACATGCCTGCATCTAAGTGGCCAATATTTTCAAGCGTATTTTCAACTGGCATCATTCC
GTACGAGCTGACCCCTGAGCAGTTGAAACGCAAATTGAATATGCGCTATTGGCCTATTGCTAGCGGCGCGGCAGCGAGTA
TATTATTAGTTAGCGGCATTGTATTTGGTTATCAGCAGCAACAAGAAACCATCGCCACTTTGGAAACCGTTATTAGCAAG
CAAAATGATCAAATTGCCGGACAAGAGTGGCAGCTTGATACGCCAGCAGAAAAAAGTTTGGCACTGATTGATAGTGAAAA
AAGCTTACATCCAGTCGTTAAGGCTGGCTTATTACGCTTACACAAGACCTATATTCTCAGCGAGTTTGAATCTGAAATTG
ATCAGGTTTTGAATTCTGATGCCAACAACTACCCGAATTATGATGAAATTGAGTCCATACTTGATAAAGCAAAAACCTAC
TACCCTGACTCACATAAAATAGAAGTTCTTGCCTTAGATATTCAGTCAAGCAAGCACTCCACGTTACTTTCGATAGCAAG
GCAAATCAACAGTCAACTTGAAAAGTCTCGGTACAGTAAAGAAGACGGCAGTAAATCCATCTATGAGCTCAAGGATGAGT
TAAATCAGATTCATCAAGGTTATCCTTTCACGCCAAGTTCGTTATCTAGCGATGTTTTCGGTCAGCACCTCACTGAAGCA
CTCAAAAAGAAAGACGCAGCTGCACTTGTTGCTCTTATTAAGGTTGGGAACACCTTCTTTTCAAACGTAGAAGAGCATCA
AGAGAACTTACAGATAAGTAATGCGATGAAAGATGCAGTGCTGGAAATGAAATTATATGAGACAGCGCTAGATTCCAACA
GCCCTCTACCGTTTCCAGAAGATGCCGCTAGAATCCTGTTCCAAGAAGAGTTTGAAAACTTTTATTCTCGCCTAGAAAAG
GCAAGGACGACAACTCATCTCGATAACTTGGTGAAGGATATGGATCTGTTCTCGAAAAACTTTCCTATTGGTTTTCAGGA
TGTTAACGATTTAAGATTCAAAACTGCTGATAAATACTTACGCTTCTCCGATATATTACTTAATAAGAGAAGGACTTCAA
GCGCAAGACGCGCTATGAAGAAAGCCAACGAGCTTATGAAGCAAATAGAACAGGACTCTAAGCAAAGCTAG