Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00794 experimental
T6SS type Type i5
Strain Vibrio alginolyticus EPJS
Replicon (GenBank) chromosome (FJ790777)
Location 1..31102
Function anti-bacterial
Comment In-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability. The interbacterial killing ability of V. alginolyticus EPGS is mediated by T6SS2.
References
[1] Sheng L et al (2012) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 162(2-4):652-62. [PudMed:23021863]
[2] Liu H et al (2012) Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 55(1):73-81. [PudMed:22563695]
[3] Zhen Yang, Xiaohui Zhou , Yue Ma, Mian Zhou, Matthew K Waldor, Yuanxing Zhang, Qiyao Wang et al (2018) Serine/threonine Kinase PpkA Coordinates the Interplay Between T6SS2 Activation and Quorum Sensing in the Marine Pathogen Vibrio Alginolyticus. Environ Microbiol. 20(2):903-919. [PudMed:29314504]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentFHATagJTssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number21112111112111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
VEPGS_0601 192..452 + 261 225382167 hypothetical protein
VEPGS_0602 482..1288 + 807 225382168 putative two-component response regulator
VEPGS_0603 1687..2148 + 462 225382169 Ser/Thr protein kinase
VEPGS_0604 2219..2416 - 198 225382170 hypothetical protein
VEPGS_0605 2445..2780 - 336 225382171 hypothetical protein
VEPGS_0606 3227..5377 - 2151 225382172 serine/threonine protein kinase
T6CP012278 FHA VEPGS_0607 5690..6775 + 1086 225382173 type VI secretion protein FHA-domain containing protein
T6CP012279 FHA VEPGS_0608 6921..7685 + 765 225791094 hypothetical protein
T6CP010938 TssK VEPGS_0609 8163..9452 + 1290 225791095 hypothetical protein
T6CP010939 TssL VEPGS_0610 9464..10756 + 1293 225382176 hypothetical protein
T6CP010940 TssM VEPGS_0611 10773..14291 + 3519 225382177 type VI secretion system IcmF-related protein
VEPGS_0612 14273..14968 + 696 225382178 hypothetical protein
VEPGS_0613 14978..15772 + 795 225382179 serine/threonine protein phosphatase
T6CP010941 TssA VEPGS_0614 15765..16880 + 1116 225382180 hypothetical protein
T6CP010942 TssB VEPGS_0615 16896..17426 + 531 225382181 hypothetical protein
T6CP010943 TssC VEPGS_0616 17426..18919 + 1494 225382182 hypothetical protein
T6CP010944 TssC VEPGS_0617 18984..20483 + 1500 225382183 hypothetical protein
T6CP012878 TagJ VEPGS_0618 20494..21288 + 795 225382184 hypothetical protein
T6CP010945 TssE VEPGS_0619 21296..21760 + 465 225382185 hypothetical protein
T6CP010946 TssF VEPGS_0620 21757..23580 + 1824 225382186 hypothetical protein
T6CP010947 TssG VEPGS_0621 23577..24245 + 669 225382187 hypothetical protein
T6CP010948 TssH VEPGS_0622 24552..27119 + 2568 225382188 putative ClpA/B-type chaperone
T6CP010949 TssD VEPGS_0623 27328..27807 + 480 225382189 uncharacterized hemolysin-coregulated protein
T6CP010950 TssI VEPGS_0624 27857..28789 + 933 225382190 Rhs element Vgr protein
T6CP010951 TssI VEPGS_0625 28759..29784 + 1026 225791096 hypothetical protein
VEPGS_0626 30087..30926 + 840 225382192 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0257 experimental VEPGS_0614 (PppA) protein Tpp Vibrio alginolyticus strain EPJS chromosome (FJ790777) 15765..16880 (+) 225382180 LuxR (↓)
REG0258 experimental VEPGS_0606 (PpkA2) protein Tpp Vibrio alginolyticus strain EPJS chromosome (FJ790777) 3227..5377 (-) 225382172 LuxR (↓); VEPGS_0610 (DotU2) (↓)
REG0259 experimental VEPGS_0607 (Fha2) protein Tpp Vibrio alginolyticus strain EPJS chromosome (FJ790777) 5690..6775 (+) 225382173 VEPGS_0610 (DotU2) (↓)
REG0260 experimental EPGS_00471 (VtsR) protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790777) - - LuxO (↓)
REG0262 experimental luxR protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790777) - - T6SS2 transcript and expression (↓)
REG0267 experimental LuxO protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790777) - - Unknown (↑)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01824 experimental VEPGS_0623 (hcp2) - - Vibrio alginolyticus strain EPJS chromosome (FJ790777) 27328..27807 (+) 225382189

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00348 experimental EPGS_03541 (rhsI1) - Vibrio alginolyticus strain EPJS chromosome (FJ790777) - -

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector