Detailed information of component protein


Summary

Component ID T6CP010941
Component protein type TssA
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (372 a.a.); Nucleotide sequence (1116 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0614
Coordinate (Strand) 15765..16880 (+)
NCBI ID 225382180
GenBank FJ790777
Uniprot ID C0LZQ4_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2.5e-32 13..142
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-371 MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTNQSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDDDGIETRISAIIGLNGIDSEGTLIFPIACIPLTDLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEAVGDVTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAEKSYFRLVGIAEQNEN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9876 - -


  Protein sequence: 372 a.a.    

>T6CP010941 FJ790777:15765-16880 [Vibrio alginolyticus] [TssA]
MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTN
QSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDDDGIETRISAIIGLNGIDSEGTLIFPIACIPLT
DLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEAVGD
VTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRE
SEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAEKSYFRLVGIAEQNEN*

  Nucleotide sequence: 1116 bp    

>T6CP010941 FJ790777:15765-16880 [Vibrio alginolyticus] [TssA]
ATGAGTAATGAAATGTTTGATTGGTATGAACAAGCGTTGGCTCCTATATCTCAAGAAAATCCTACAGGTATTGATCCTAG
AGAGGATGTATCACCACAGTCTGCTTACTATCGGTTGAAAGATCAGCGTATGGCCGCCCGCAACGCTGAACGTAATGCTC
TTATAGAAGAAGAAAGTATCTATACCCACAGTAATTTATGGCGAGTATTTATTGAAGATGTCCCAGAAATACTCACGAAT
CAGTCTAAAGATTTGGAATTTGTTGCCTGGCTTATAGAAGCCCTAACTCGATTGTATGGTTTTCGTGGTATGGGCGTTGG
CTACAAACTCGCAACCAGTTTAATTGAGAACTTTTGGGATGATCTGTACCCAATGCCTGATGATGATGGTATTGAGACAA
GAATATCGGCGATTATTGGCCTCAATGGTATCGATAGTGAAGGAACACTTATTTTTCCAATTGCTTGTATACCATTGACT
GACTTAGGCGTGGAGCAAGCTTATGCCTATTGGGAATATCAGCAAGCTATAGAGCTTGAGCGCTTAGACGAAGATAAGCG
CCGAAATCGAGTCGAGCTTGGGGCTGTTGAGCTATCTGAAATTATAACTACGGTTAAGTCGACTTCTGATACGTTTTATC
AAGAATTAATCACGGATTTGGAGTTCGCTACAGAATCATTTAATGAATTCAGTCAGCGATTAGACGAAGCGGTCGGTGAT
GTGACTCCTAGCTCTTATATATCAAAAAGACTCGAATCAATACACTCGGCATTAAAGCACCTTCTAGGAGATCGTTTCAA
TCAACCACAAGCCGTGTTTGAAGAGCTAGAGGATCTTGATGAATCTTCTTCTGAACAACCGTTAAGTTCACAAAGTTGTG
ATGCATTATTAGCGACAAACATGAAATCACGAGAGCAGGCAATTAGACAATTGCAGCAAGTAGCTGATTTCTTCCGAGAG
AGTGAACCTCACTCACCAGTTTCTTACTCCATCGAACAGATCATTCGTTGGTGTGGGATGCCTCTGCCGGAGTTACTGGC
GGAACTTATCTCCGACGGGGATGCAGAGAAAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATTAA