Detailed information of component protein


Summary

Component ID T6CP010940
Component protein type TssM
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (1173 a.a.); Nucleotide sequence (3519 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0611
Coordinate (Strand) 10773..14291 (+)
NCBI ID 225382177
GenBank FJ790777
Uniprot ID C0LZQ1_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 2.6e-86 193..450
IcmF-related PF06761.14 2.5e-75 503..803
IcmF_C PF06744.14 1.5e-18 1049..1149
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MRIKEIGRIFTQ
Transmembrane helix 13-32 RWFLGLIGVAACSIFIWVVG
Outside 33-46 PLITIAGYEPLKSD
Transmembrane helix 47-64 FQRLVTILVLVLLWAIFN
Inside 65-445 VTKQHKQKVKEDESIQTLLEVDSQSDKEAASEIDLMRERIEQAVKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLGDSLGSDSIAGVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASVMSQTRTERSLHARAIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDARMLRILETEDDLDKRALIFEFPKQLRVLQANLDEFLAEIFAQNSFEEPALIRGVFMLSSVQEGLPVDKLMNESTNGLGLNRLALAKSQTGSHSYFVKNLFDKVIFKEQLLGTVNRHYQKQSGWMRR
Transmembrane helix 446-463 GVYIGCTTLLLGASALWF
Outside 464-1172 MSYKWNSRLIVDTNEQASHIDAMIGAQSLDFEADIVSAVETLDRLMTLPLGKQSNYGDADSVKRFGLYQGDKVSQAASNAYSDALTQYYAPLLLDSLVSEMESNQQHREYLYETLKTYLMLFNPDKYQQDEVLAWFNFYFERQYPGELNVDLRRRLLQHTQYLLGNDGQGFTYNTAAVTSAREVLTQMSLPERAYQRMKIQFSKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTVKSLMEENWVYGDDLKAHEIDQDSAIQGVQSRYYQDYVNEWKTLIEDIQLKQAPSLELATEQARVLSGVERPIESLLRAIQKEVALSKVSLSENQKAASEVVGKVAKVKFSNTADRLDMYLPEENGFNVALPGKEVESHFSEILRLGEQDFDDIQQAMVNLRSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKELLPRPFNQWLGELSQESVKFAESGSKDHLNQLWVTQVVRPYQRTIAGRYPFEPNASREVRLKDFKRFFGYGGTLDAFFQEYLEAFVDTSKSRWRLEKEIGVRPETLMVFQRAQRIRQSFFESDNSLRVEFGMKPVYLDQHITRFVLELGGQDLVYKHGPARAKSLSWPAGQDQTRIVFTPPESKREIAHTYEGEWGIFKLLDQSLKARPESRKDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPRTL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.5466 - -


  Protein sequence: 1173 a.a.    

>T6CP010940 FJ790777:10773-14291 [Vibrio alginolyticus] [TssM]
MRIKEIGRIFTQRWFLGLIGVAACSIFIWVVGPLITIAGYEPLKSDFQRLVTILVLVLLWAIFNVTKQHKQKVKEDESIQ
TLLEVDSQSDKEAASEIDLMRERIEQAVKVVTKTHKGKRSLYDLPWYVLIGPPGTGKTTVLKQSGLEFPLGDSLGSDSIA
GVGGTRHCDWWFANKAVLIDTAGRYTTQDSQEKVDSKAWHGFLGLLKKYRTQRPINGAIVTISLASVMSQTRTERSLHAR
AIKSRLQELKNQLGMQFPIYVLLTKMDLVAGFNEFFADLSKEEREELFGFMFPREVDDERGVISLFNKEFHGMLERLDAR
MLRILETEDDLDKRALIFEFPKQLRVLQANLDEFLAEIFAQNSFEEPALIRGVFMLSSVQEGLPVDKLMNESTNGLGLNR
LALAKSQTGSHSYFVKNLFDKVIFKEQLLGTVNRHYQKQSGWMRRGVYIGCTTLLLGASALWFMSYKWNSRLIVDTNEQA
SHIDAMIGAQSLDFEADIVSAVETLDRLMTLPLGKQSNYGDADSVKRFGLYQGDKVSQAASNAYSDALTQYYAPLLLDSL
VSEMESNQQHREYLYETLKTYLMLFNPDKYQQDEVLAWFNFYFERQYPGELNVDLRRRLLQHTQYLLGNDGQGFTYNTAA
VTSAREVLTQMSLPERAYQRMKIQFSKSHVPSFRLTDVLGPKGLEQFERASGKPLSQGISGFYTYNGFHSIFQIQINRTV
KSLMEENWVYGDDLKAHEIDQDSAIQGVQSRYYQDYVNEWKTLIEDIQLKQAPSLELATEQARVLSGVERPIESLLRAIQ
KEVALSKVSLSENQKAASEVVGKVAKVKFSNTADRLDMYLPEENGFNVALPGKEVESHFSEILRLGEQDFDDIQQAMVNL
RSYLSDLSSSGNNQKIAYKSILDGTVTQDVAASFARAKELLPRPFNQWLGELSQESVKFAESGSKDHLNQLWVTQVVRPY
QRTIAGRYPFEPNASREVRLKDFKRFFGYGGTLDAFFQEYLEAFVDTSKSRWRLEKEIGVRPETLMVFQRAQRIRQSFFE
SDNSLRVEFGMKPVYLDQHITRFVLELGGQDLVYKHGPARAKSLSWPAGQDQTRIVFTPPESKREIAHTYEGEWGIFKLL
DQSLKARPESRKDNIVMIDLKGNKVQLELIPSSAINPFWSNEMERFRCPRTL*

  Nucleotide sequence: 3519 bp    

>T6CP010940 FJ790777:10773-14291 [Vibrio alginolyticus] [TssM]
ATGCGTATTAAAGAAATTGGCAGAATATTTACTCAAAGATGGTTTCTCGGTCTTATCGGTGTCGCTGCTTGTTCTATTTT
CATTTGGGTTGTTGGTCCACTTATTACGATAGCGGGGTATGAGCCCCTAAAGAGTGACTTTCAACGGCTAGTCACTATTC
TTGTGTTAGTGTTGCTTTGGGCGATATTTAACGTTACCAAGCAGCATAAGCAGAAGGTGAAAGAAGACGAAAGTATTCAA
ACGTTGCTTGAGGTTGATAGCCAATCAGATAAAGAAGCGGCAAGTGAAATTGACTTAATGCGCGAGCGTATTGAGCAAGC
TGTCAAAGTAGTGACGAAAACACACAAAGGCAAGAGAAGTTTGTATGACTTACCGTGGTATGTATTGATTGGCCCTCCTG
GAACGGGGAAAACAACCGTATTAAAGCAGTCGGGGTTAGAGTTTCCATTAGGAGACAGCCTTGGCTCTGACTCTATTGCC
GGTGTAGGTGGCACTCGCCACTGTGATTGGTGGTTTGCAAATAAAGCAGTTCTTATAGATACGGCTGGCCGGTATACGAC
ACAAGACAGTCAAGAAAAGGTCGACTCTAAAGCGTGGCATGGTTTCTTAGGATTGTTGAAAAAGTATAGAACGCAAAGAC
CAATTAATGGTGCGATTGTTACGATAAGCTTAGCCAGCGTGATGTCTCAGACTCGAACCGAGCGAAGTCTTCATGCTCGA
GCAATTAAGTCGAGACTTCAAGAACTGAAAAACCAGTTAGGCATGCAGTTTCCGATTTACGTATTGCTCACCAAAATGGA
TTTAGTTGCCGGTTTCAATGAGTTCTTTGCCGATTTAAGTAAGGAAGAGCGAGAGGAATTATTTGGTTTCATGTTCCCTC
GTGAAGTGGATGATGAAAGAGGCGTTATTTCTCTATTCAACAAAGAATTTCATGGCATGTTGGAGCGCCTTGATGCCCGT
ATGCTTAGAATACTTGAAACAGAGGACGATTTAGATAAACGCGCGCTTATCTTTGAATTTCCAAAGCAATTAAGAGTGCT
ACAAGCTAATCTTGACGAGTTTTTAGCTGAAATTTTTGCACAAAACAGTTTTGAAGAGCCTGCCTTGATTCGTGGTGTGT
TTATGTTGAGTTCGGTTCAAGAAGGCTTACCAGTCGATAAGCTGATGAATGAATCAACCAATGGACTTGGGTTAAATCGT
TTAGCTCTAGCAAAGAGTCAGACTGGTTCTCATAGTTATTTCGTAAAGAATCTTTTTGACAAGGTTATTTTCAAAGAGCA
ACTGTTAGGAACAGTTAATCGTCACTATCAAAAGCAGTCAGGTTGGATGCGTCGAGGTGTTTATATTGGATGTACAACGC
TACTGCTAGGAGCTTCTGCGCTTTGGTTTATGAGTTATAAGTGGAACTCACGGTTAATTGTCGACACCAATGAACAAGCT
AGCCATATTGATGCAATGATTGGCGCTCAAAGTTTAGATTTTGAAGCGGATATTGTTTCAGCTGTAGAAACATTAGATAG
GTTGATGACGCTTCCTCTCGGAAAGCAAAGCAACTACGGTGATGCCGACTCTGTAAAGAGGTTTGGTCTCTATCAAGGAG
ACAAAGTATCTCAGGCGGCAAGCAATGCATATTCTGATGCTTTGACCCAATATTACGCCCCTTTGCTTCTTGATAGCCTG
GTGTCAGAAATGGAGAGCAATCAACAGCATCGTGAGTATTTGTACGAAACGTTAAAAACGTATTTGATGTTATTCAATCC
AGACAAATATCAACAAGATGAAGTGTTGGCTTGGTTTAACTTTTATTTCGAACGTCAATATCCGGGTGAGCTAAACGTAG
ACTTAAGAAGACGCTTGCTACAGCACACTCAATATTTATTAGGCAACGATGGACAAGGCTTTACTTACAACACTGCTGCT
GTGACGAGTGCTCGAGAAGTGTTGACTCAAATGTCTCTACCTGAACGTGCTTATCAGCGAATGAAAATACAATTTTCTAA
GAGTCATGTACCTTCATTTCGCTTGACTGATGTATTGGGACCTAAAGGGCTTGAGCAGTTCGAGCGAGCGAGTGGAAAAC
CGCTATCACAAGGCATTTCGGGCTTCTACACGTACAATGGTTTTCATAGCATTTTTCAGATTCAGATCAACCGTACAGTA
AAAAGCCTAATGGAAGAGAACTGGGTTTATGGTGACGATCTTAAGGCCCATGAAATTGATCAAGATAGTGCCATCCAAGG
CGTTCAGTCTCGATACTATCAAGATTATGTTAATGAATGGAAAACGTTGATCGAAGATATTCAATTGAAGCAGGCTCCGA
GCCTAGAACTTGCGACAGAGCAAGCGAGGGTTTTATCTGGAGTCGAAAGACCAATCGAATCTTTGTTGAGAGCAATCCAA
AAAGAGGTTGCTCTATCAAAAGTATCATTGTCAGAAAATCAAAAAGCCGCTTCTGAAGTTGTTGGTAAAGTTGCCAAAGT
TAAGTTTTCAAATACGGCAGATAGACTAGACATGTATTTACCTGAAGAAAACGGTTTCAATGTTGCTCTGCCAGGTAAAG
AAGTCGAATCGCATTTTTCAGAGATCTTACGCCTTGGTGAGCAAGACTTTGATGATATTCAACAAGCAATGGTTAACTTG
CGTTCGTATTTAAGTGACTTATCAAGCTCTGGTAACAATCAGAAAATTGCCTACAAAAGTATTTTAGATGGAACCGTTAC
ACAAGATGTCGCAGCTTCCTTTGCTCGTGCTAAAGAACTTTTGCCGAGACCATTTAATCAATGGCTAGGTGAGCTTTCTC
AAGAGTCGGTTAAGTTTGCGGAGAGCGGTTCGAAGGATCATCTAAATCAACTTTGGGTAACACAGGTGGTCCGCCCTTAT
CAGCGGACTATTGCTGGCAGATATCCATTCGAGCCCAACGCGAGCAGAGAAGTTAGATTGAAAGACTTTAAGCGATTCTT
TGGTTATGGAGGTACGCTTGACGCATTCTTCCAAGAATACCTCGAAGCGTTTGTGGATACTTCGAAAAGTCGTTGGCGAT
TAGAAAAAGAAATTGGAGTTCGTCCAGAAACACTAATGGTCTTTCAAAGAGCGCAACGTATCAGGCAGTCTTTCTTTGAA
TCCGATAATTCCTTACGCGTAGAGTTTGGGATGAAGCCTGTTTATCTAGACCAGCACATTACTCGATTTGTCTTAGAGTT
GGGTGGACAAGATTTGGTTTATAAGCATGGACCAGCAAGAGCTAAATCGTTGAGTTGGCCTGCCGGTCAAGATCAGACTC
GAATTGTGTTCACACCACCTGAATCTAAACGCGAAATTGCGCATACCTATGAAGGTGAGTGGGGCATATTCAAGCTTTTG
GATCAATCACTTAAAGCAAGGCCAGAATCGCGTAAAGACAATATAGTTATGATTGATTTAAAGGGAAATAAGGTGCAACT
AGAGCTTATTCCTTCTAGTGCCATTAATCCGTTTTGGTCAAATGAAATGGAGAGGTTCAGATGTCCACGAACCTTATAA