Detailed information of component protein


Summary

Component ID T6CP010939
Component protein type TssL
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0610
Coordinate (Strand) 9464..10756 (+)
NCBI ID 225382176
GenBank FJ790777
Uniprot ID C0LZQ0_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 3.7e-55 51..252
OmpA PF00691.22 1.4e-13 322..420
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-229 MEQTIVKPTPGGRAHASKPQSEKSLDNTVVISKSPELVNNDSVVAYGNNSLLAEANGLLSIIGQIRSTATHSDPLFLKETLAQKLRDYENRSRQHDVDLDTIDTARYCLCCSLDEVVLNTNWGGHSFWSHDSLLSSFYASSQGGEAFFKHLDKCMAKPDANLDLLELMYVCLSLGFLGQYRLEKNGLESHRKLRRQVIATLKSYGRGVPQELSNSIDQHILNGSKASDR
Transmembrane helix 230-252 APLWVVCSVTAALLVCIYMYFSY
Inside 253-430 ELNKASNRTFSQLVNLIQPQQLNQDATSESKSAPIAEKISMYLATEIGKGLINVEPLQDRVRISLKSQDLFESGSADVVAYIQPVISKLARTLESTQGKIIVTGHTDDRPIFTSKYPSNWHLSLARATAMSEQLISNSALEGRVIPEGLGDARPLVENSSDENRAINRRIEIDLLVAN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9029 - -


  Protein sequence: 431 a.a.    

>T6CP010939 FJ790777:9464-10756 [Vibrio alginolyticus] [TssL]
MEQTIVKPTPGGRAHASKPQSEKSLDNTVVISKSPELVNNDSVVAYGNNSLLAEANGLLSIIGQIRSTATHSDPLFLKET
LAQKLRDYENRSRQHDVDLDTIDTARYCLCCSLDEVVLNTNWGGHSFWSHDSLLSSFYASSQGGEAFFKHLDKCMAKPDA
NLDLLELMYVCLSLGFLGQYRLEKNGLESHRKLRRQVIATLKSYGRGVPQELSNSIDQHILNGSKASDRAPLWVVCSVTA
ALLVCIYMYFSYELNKASNRTFSQLVNLIQPQQLNQDATSESKSAPIAEKISMYLATEIGKGLINVEPLQDRVRISLKSQ
DLFESGSADVVAYIQPVISKLARTLESTQGKIIVTGHTDDRPIFTSKYPSNWHLSLARATAMSEQLISNSALEGRVIPEG
LGDARPLVENSSDENRAINRRIEIDLLVAN*

  Nucleotide sequence: 1293 bp    

>T6CP010939 FJ790777:9464-10756 [Vibrio alginolyticus] [TssL]
ATGGAACAAACGATCGTAAAACCAACACCGGGTGGAAGAGCACACGCATCGAAACCACAATCAGAAAAGTCACTGGATAA
TACCGTAGTTATCTCAAAAAGCCCTGAATTGGTGAACAATGATTCAGTGGTCGCTTATGGGAATAACTCTCTACTAGCAG
AGGCGAATGGCCTGTTATCTATTATCGGTCAAATTCGGTCGACAGCGACTCATTCCGATCCGTTGTTTCTAAAAGAGACA
TTGGCACAAAAGCTTAGAGATTATGAGAACCGATCACGTCAGCATGACGTTGATTTGGATACCATTGATACAGCTCGTTA
TTGTTTATGCTGTTCACTAGATGAAGTTGTCCTTAACACCAATTGGGGTGGGCATTCATTCTGGAGTCACGATTCATTAC
TCAGCTCCTTCTATGCTAGCAGTCAAGGTGGGGAAGCGTTCTTTAAGCATCTTGATAAGTGCATGGCGAAACCAGATGCG
AACTTAGATTTGCTTGAACTAATGTATGTGTGCTTATCTCTCGGGTTTCTGGGTCAATACCGTTTAGAAAAAAATGGACT
GGAATCGCACCGGAAATTGAGAAGACAGGTTATTGCTACTCTTAAGTCTTATGGGAGAGGAGTCCCTCAGGAGCTAAGTA
ATAGTATCGACCAACACATTTTGAATGGCTCAAAGGCTTCTGATAGAGCCCCTCTTTGGGTTGTATGTTCTGTAACGGCG
GCATTATTGGTCTGCATATATATGTATTTTAGCTATGAGCTAAATAAGGCATCCAATCGCACCTTTTCTCAATTAGTCAA
TTTGATTCAACCGCAACAACTTAACCAAGATGCAACGTCAGAATCAAAATCAGCGCCAATTGCAGAAAAGATTTCAATGT
ATCTAGCAACTGAGATCGGTAAGGGGCTAATCAATGTTGAGCCGCTGCAAGACAGGGTAAGAATTTCGTTAAAGTCTCAA
GATCTATTTGAATCAGGTAGTGCTGATGTTGTCGCTTACATCCAACCCGTTATTTCTAAGCTTGCGAGAACATTGGAGTC
GACCCAAGGCAAGATCATTGTTACTGGTCACACCGACGATAGGCCTATTTTCACGAGTAAATATCCCTCGAACTGGCACC
TATCATTAGCTCGAGCCACTGCTATGTCTGAACAACTGATCTCGAATAGTGCATTAGAAGGGCGAGTGATACCCGAGGGA
TTGGGGGATGCAAGACCATTAGTTGAAAACAGTAGTGATGAAAACCGAGCTATTAACCGCCGGATTGAAATCGACCTTCT
TGTCGCGAATTAA