Detailed information of component protein


Summary

Component ID T6CP010948
Component protein type TssH
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (856 a.a.); Nucleotide sequence (2568 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0622
Coordinate (Strand) 24552..27119 (+)
NCBI ID 225382188
GenBank FJ790777
Uniprot ID C0LZR2_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.7e-38 587..753
AAA_lid_9 PF17871.3 7e-28 354..444
ClpB_D2-small PF10431.11 2.5e-10 760..834
AAA PF00004.31 7.1e-09 213..326
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-855 MASLSLSALVERLSPQAKETLEQAAAMASSRTHHSIEMSHWLMMIIRHPDDIYSEVFDEFDVDKIKIETDIQKALEKYKTGCQSVPGLSAHTVTVMQNALLSATIEFNQEQLGCIHLLFAYLSDDTLVNMAGSISPELNKIEPSRLLNFKESNAPQSRSNDLAQSNANSALNQFTTDLTEQAREGKIDPVIGRDNEIRLMIDILLRRRQNNPILTGEAGVGKTAVVEGLAQKIIDGDVPPALQNVAIKSLDLALLQAGAGMKGEFENRLKNLVKEVNDAPQPIILFIDEAHGLIGGGGQAGQGDAANILKPALARGELRTIAATTWAEYKKYFEKDAALTRRFQVVKVDEPAPELAIEMLRGLLDVMAQHHDVHITNQALNAAVQLSARYINGRQLPDKAVSVLDTACSRVTLSQSSTPGGLDAKRNKLKAKQAEYEHLSQENRLGASLDVQLQGVRKEIDALSNDIIELEVLWEKEKEWVSEAKSLRTQIIQEGSETARDKLQELENNLSANSQPLVFSHVNEALIAEVISDWTGIPLGKLQDDEIQAVLGLKESLCKRVVGQDHALELMSEVIKTASAQLTDETRPNGVFLLTGPSGVGKTESALAIAEKVYGSEENVTTINMSEFKEEHKVSLLLGSPPGYVGYGEGGVLTEAVRRKPYSVILLDEMEKAHPGVQDIFYQVFDKGSIKDGEGRDIDFKNTIIIMTANTGTETTMSLYSDPDLAPEPSALKDALRDDLLVDFKPAFLGRVNVLPYLPLSRKVLIEITKLKLDKITTRIRKHYNADLFFDEELINDIVDNSNESGSGARVVQTTIENTLLPKISHEILHAILNDKTFDEIIVKGTTSGLDVSMV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9723 - -


  Protein sequence: 856 a.a.    

>T6CP010948 FJ790777:24552-27119 [Vibrio alginolyticus] [TssH]
MASLSLSALVERLSPQAKETLEQAAAMASSRTHHSIEMSHWLMMIIRHPDDIYSEVFDEFDVDKIKIETDIQKALEKYKT
GCQSVPGLSAHTVTVMQNALLSATIEFNQEQLGCIHLLFAYLSDDTLVNMAGSISPELNKIEPSRLLNFKESNAPQSRSN
DLAQSNANSALNQFTTDLTEQAREGKIDPVIGRDNEIRLMIDILLRRRQNNPILTGEAGVGKTAVVEGLAQKIIDGDVPP
ALQNVAIKSLDLALLQAGAGMKGEFENRLKNLVKEVNDAPQPIILFIDEAHGLIGGGGQAGQGDAANILKPALARGELRT
IAATTWAEYKKYFEKDAALTRRFQVVKVDEPAPELAIEMLRGLLDVMAQHHDVHITNQALNAAVQLSARYINGRQLPDKA
VSVLDTACSRVTLSQSSTPGGLDAKRNKLKAKQAEYEHLSQENRLGASLDVQLQGVRKEIDALSNDIIELEVLWEKEKEW
VSEAKSLRTQIIQEGSETARDKLQELENNLSANSQPLVFSHVNEALIAEVISDWTGIPLGKLQDDEIQAVLGLKESLCKR
VVGQDHALELMSEVIKTASAQLTDETRPNGVFLLTGPSGVGKTESALAIAEKVYGSEENVTTINMSEFKEEHKVSLLLGS
PPGYVGYGEGGVLTEAVRRKPYSVILLDEMEKAHPGVQDIFYQVFDKGSIKDGEGRDIDFKNTIIIMTANTGTETTMSLY
SDPDLAPEPSALKDALRDDLLVDFKPAFLGRVNVLPYLPLSRKVLIEITKLKLDKITTRIRKHYNADLFFDEELINDIVD
NSNESGSGARVVQTTIENTLLPKISHEILHAILNDKTFDEIIVKGTTSGLDVSMV*

  Nucleotide sequence: 2568 bp    

>T6CP010948 FJ790777:24552-27119 [Vibrio alginolyticus] [TssH]
ATGGCTTCACTCTCACTTTCAGCGTTGGTCGAAAGATTGTCTCCTCAGGCTAAGGAAACACTTGAACAGGCAGCTGCGAT
GGCAAGCTCCCGAACTCATCACAGTATTGAAATGTCTCACTGGTTAATGATGATTATCCGCCATCCTGACGATATTTACT
CTGAAGTTTTTGACGAATTTGATGTCGATAAAATCAAGATTGAAACCGATATTCAAAAGGCATTAGAAAAATACAAAACA
GGATGCCAGTCGGTGCCTGGGCTATCAGCTCATACGGTGACTGTTATGCAAAATGCTTTGTTATCTGCAACCATTGAATT
CAATCAAGAACAGTTGGGGTGCATCCATCTTTTATTTGCCTACTTGTCTGATGATACGTTAGTCAACATGGCTGGTTCGA
TTAGTCCTGAACTAAACAAGATAGAACCATCTCGCTTATTGAACTTTAAAGAAAGTAATGCGCCTCAATCAAGATCGAAT
GACTTAGCGCAGTCAAACGCGAATTCCGCCTTGAATCAATTTACAACAGACCTTACTGAGCAAGCTAGGGAAGGTAAAAT
AGATCCGGTGATTGGTAGAGATAATGAAATTCGCCTAATGATCGATATTTTATTAAGACGCCGACAAAACAACCCGATTC
TTACGGGAGAAGCTGGTGTTGGAAAAACAGCCGTTGTTGAAGGTTTGGCGCAAAAGATCATTGATGGCGATGTACCTCCC
GCTCTGCAAAATGTGGCAATTAAGTCTCTGGATTTGGCGTTACTGCAAGCCGGCGCAGGCATGAAAGGTGAGTTTGAAAA
CAGGCTTAAGAACCTTGTTAAGGAAGTAAACGACGCTCCACAGCCTATTATATTGTTCATTGATGAAGCTCATGGCCTAA
TTGGCGGTGGTGGGCAAGCGGGCCAAGGTGATGCGGCGAATATTCTGAAGCCAGCATTAGCGAGAGGTGAACTCAGAACG
ATCGCAGCAACAACGTGGGCAGAGTATAAAAAGTATTTTGAAAAAGATGCAGCTTTAACTAGGCGTTTTCAAGTCGTAAA
GGTTGATGAGCCTGCCCCTGAACTCGCGATCGAAATGTTACGAGGCTTACTTGATGTAATGGCACAACACCACGATGTTC
ATATCACTAACCAAGCTCTCAATGCAGCGGTCCAACTCTCAGCTCGATACATTAATGGTCGTCAACTGCCAGATAAAGCG
GTTTCTGTTTTGGATACGGCCTGCTCTCGAGTTACGTTAAGTCAGTCATCTACACCGGGAGGCCTAGATGCGAAAAGAAA
TAAACTTAAAGCAAAGCAAGCTGAGTATGAGCATCTTAGCCAAGAGAATCGATTAGGAGCATCTCTTGATGTGCAGCTCC
AAGGTGTCCGAAAAGAGATTGATGCGCTATCTAATGACATTATCGAATTAGAGGTACTTTGGGAAAAAGAAAAAGAATGG
GTAAGTGAAGCTAAGTCACTTAGAACCCAAATAATCCAGGAGGGTTCTGAAACTGCTAGAGACAAGTTACAAGAGCTTGA
AAATAACCTTTCCGCTAACTCACAGCCGTTAGTTTTTTCGCATGTAAATGAAGCGCTAATCGCAGAAGTTATTTCGGATT
GGACCGGTATACCGCTGGGTAAACTTCAGGATGATGAAATCCAAGCAGTATTGGGTTTGAAAGAAAGTTTGTGTAAGCGA
GTTGTCGGGCAGGATCACGCTTTAGAGCTCATGTCTGAAGTGATAAAAACCGCGAGTGCGCAGCTCACGGATGAGACACG
ACCAAATGGTGTATTTCTCCTAACCGGACCAAGCGGAGTGGGTAAAACCGAATCGGCTTTGGCGATTGCAGAGAAAGTGT
ATGGTAGTGAAGAAAATGTCACGACTATCAATATGTCGGAATTTAAGGAAGAGCATAAAGTCTCATTGCTATTAGGTTCT
CCTCCTGGTTATGTCGGTTATGGAGAAGGAGGTGTACTGACTGAAGCTGTTAGACGTAAACCTTATAGTGTAATTCTCCT
CGATGAAATGGAAAAAGCGCACCCTGGTGTTCAAGATATTTTTTATCAGGTATTTGATAAAGGCTCAATTAAAGACGGTG
AAGGTAGAGATATAGACTTTAAAAACACTATTATTATTATGACCGCGAACACAGGGACCGAAACGACGATGTCTTTGTAT
TCTGATCCTGATTTAGCTCCTGAGCCGTCAGCACTAAAAGATGCTTTACGCGATGATTTGTTGGTTGACTTTAAACCAGC
ATTTTTAGGGCGTGTGAATGTATTACCTTATTTGCCTCTATCAAGAAAAGTATTGATAGAAATAACAAAATTAAAGTTGG
ATAAAATTACGACTCGTATTCGTAAGCACTATAACGCTGACCTGTTTTTTGATGAGGAACTAATTAATGATATTGTAGAC
AATAGCAATGAGTCTGGGAGTGGTGCGCGTGTGGTACAAACAACCATAGAAAATACATTGCTTCCGAAGATTTCTCATGA
AATATTGCATGCAATTCTTAATGATAAGACGTTTGATGAAATTATTGTTAAAGGTACAACTAGTGGACTTGATGTCTCAA
TGGTTTAA