Detailed information of component protein


Summary

Component ID T6CP010942
Component protein type TssB
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (177 a.a.); Nucleotide sequence (531 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0615
Coordinate (Strand) 16896..17426 (+)
NCBI ID ACN89321.1
GenBank FJ790777
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VipA PF05591.14 6.2e-62 9..161
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-176 MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMNPSVKFKVANKLANDDSQFEVDLNFKSMQDFEPAAIVNQVDPLRQLMETRNKLRDLMTKVDRSEELENILEDVLNNTDNLARLAGELDLDKKAKSDSTEGAE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9966 - -


  Protein sequence: 177 a.a.    

>T6CP010942 FJ790777:16896-17426 [Vibrio alginolyticus] [TssB]
MTSIHSKLSRVRKPRVHITYDVETEGTTLKKELPFVVGVMGDFAGQNTEALKPLKDRRFIQIDRDNFDDVLKKMNPSVKF
KVANKLANDDSQFEVDLNFKSMQDFEPAAIVNQVDPLRQLMETRNKLRDLMTKVDRSEELENILEDVLNNTDNLARLAGE
LDLDKKAKSDSTEGAE*

  Nucleotide sequence: 531 bp    

>T6CP010942 FJ790777:16896-17426 [Vibrio alginolyticus] [TssB]
ATGACGAGTATCCACTCGAAACTATCACGAGTACGGAAGCCTCGTGTCCACATTACCTATGATGTTGAAACTGAAGGTAC
AACTCTTAAGAAAGAACTGCCTTTTGTTGTAGGTGTAATGGGTGATTTCGCTGGTCAAAACACAGAAGCGTTGAAGCCAT
TAAAAGACCGCCGTTTTATTCAGATCGATCGAGATAACTTTGACGATGTACTCAAAAAAATGAATCCATCGGTGAAGTTT
AAAGTTGCGAATAAGCTTGCGAATGACGATTCACAGTTTGAGGTCGATTTAAACTTTAAATCAATGCAAGACTTTGAGCC
TGCTGCGATCGTAAATCAAGTTGATCCACTACGCCAATTGATGGAAACGCGTAATAAACTGCGTGATTTGATGACAAAAG
TTGACCGCTCAGAAGAGCTTGAAAATATCCTAGAGGATGTTTTGAACAATACGGACAATCTGGCAAGACTTGCAGGCGAG
TTAGATCTTGACAAGAAAGCGAAGTCAGACAGTACTGAAGGAGCGGAATAA