Detailed information of regulator
Summary
| Regulator ID | REG0259  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00794 (Type i5) |
| Strain | Vibrio alginolyticus strain EPJS |
| Replicon | chromosome |
| Sequence | Protein sequence (362 a.a.); Nucleotide sequence (1086 bp) |
| Description | Fha2 interacts DotU and is critical to T6SS activation |
| Reference |
External database links
| Locus tag (Gene) | VEPGS_0607 (Fha2) |
| Coordinates (Strand) | 5690..6775 (+) |
| NCBI ID | 225382173 |
| GenBank | FJ790777 |
| Uniprot ID | C0LZP7_VIBAL |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| VEPGS_0610 (DotU2) | ↓ | 225382176 |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FHA | PF00498.28 | 4.1e-14 | 32..101 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-361 | MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVVSGTHGELIKFGDKYLIKDLSTNGTFVNNAVTPVGQGNEIALSDDDMVSLGDYQISVSIQTDTDVFSLETSKSLSSAPPEVVSSPSEAEFVDLGLSASELLNESVPSDFQLDIGLVDDFIDIPKPEKQSTVSETLPKVNRQSEHLSTPNFDTNGGELAAFVRGVGIDPMMIPSDNREEWFEQLGESFSLMLVGLMETLHNRAVFKQTNRLSHTAFRKSENNPLKFSANLEDAIHNLYNRQTASFLKPNAAIKEAFNDIESHEQALMKGVEGTVSSVMSLVEPTSIYSDALAKDTVINKVIPARNMLKLEEV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9668 | - | - |
Protein sequence of regulator: 362 a.a. .
>REG0259 FJ790777:5690-6775 [Vibrio alginolyticus]
MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVVSGTHGELIKFGDKYLIKDLSTNGTFVNNA
VTPVGQGNEIALSDDDMVSLGDYQISVSIQTDTDVFSLETSKSLSSAPPEVVSSPSEAEFVDLGLSASELLNESVPSDFQ
LDIGLVDDFIDIPKPEKQSTVSETLPKVNRQSEHLSTPNFDTNGGELAAFVRGVGIDPMMIPSDNREEWFEQLGESFSLM
LVGLMETLHNRAVFKQTNRLSHTAFRKSENNPLKFSANLEDAIHNLYNRQTASFLKPNAAIKEAFNDIESHEQALMKGVE
GTVSSVMSLVEPTSIYSDALAKDTVINKVIPARNMLKLEEV*
MKLVLTITSFHKFTPEIESEFVFESSDEHAKVTFGRSEQCDWTLPDPERVVSGTHGELIKFGDKYLIKDLSTNGTFVNNA
VTPVGQGNEIALSDDDMVSLGDYQISVSIQTDTDVFSLETSKSLSSAPPEVVSSPSEAEFVDLGLSASELLNESVPSDFQ
LDIGLVDDFIDIPKPEKQSTVSETLPKVNRQSEHLSTPNFDTNGGELAAFVRGVGIDPMMIPSDNREEWFEQLGESFSLM
LVGLMETLHNRAVFKQTNRLSHTAFRKSENNPLKFSANLEDAIHNLYNRQTASFLKPNAAIKEAFNDIESHEQALMKGVE
GTVSSVMSLVEPTSIYSDALAKDTVINKVIPARNMLKLEEV*
Nucleotide sequence of regulator: 1086 bp .
>REG0259 FJ790777:5690-6775 [Vibrio alginolyticus]
ATGAAGTTGGTTTTAACCATAACAAGTTTTCACAAATTTACTCCAGAGATTGAGAGTGAGTTCGTGTTTGAATCAAGTGA
TGAACATGCCAAGGTTACATTTGGTAGGTCTGAACAATGTGATTGGACACTACCAGATCCTGAAAGAGTGGTATCCGGAA
CCCACGGAGAACTGATTAAGTTTGGTGACAAATACTTGATAAAAGACCTCTCAACTAATGGTACTTTTGTAAATAATGCT
GTAACTCCTGTGGGTCAAGGAAATGAAATAGCACTAAGCGATGACGATATGGTTAGCTTAGGGGATTATCAAATTTCAGT
TTCTATTCAAACAGATACAGATGTATTCAGTCTTGAGACAAGTAAAAGTCTTTCTAGCGCACCCCCTGAAGTAGTCTCAA
GTCCATCGGAAGCAGAATTTGTTGACCTAGGCCTTAGTGCTAGTGAACTCTTAAACGAAAGTGTTCCTTCTGACTTTCAG
CTAGATATTGGTTTAGTCGATGATTTTATTGATATCCCAAAGCCCGAAAAACAGAGCACCGTATCTGAAACTTTACCGAA
AGTTAACCGACAATCAGAACACCTATCGACTCCCAACTTTGATACCAATGGGGGTGAACTTGCGGCTTTTGTTAGAGGGG
TGGGTATAGATCCAATGATGATTCCCAGCGATAACCGAGAGGAATGGTTCGAACAACTGGGGGAGTCATTTTCTTTGATG
CTAGTTGGTTTAATGGAAACACTGCATAACAGAGCAGTGTTTAAACAAACAAATCGACTTAGTCATACCGCATTTCGTAA
GTCAGAAAATAACCCTTTAAAGTTTTCTGCAAACTTAGAAGATGCGATTCATAACTTATATAACCGTCAAACCGCTAGCT
TCTTAAAGCCAAATGCAGCCATTAAAGAAGCATTTAATGACATCGAAAGTCATGAGCAAGCCTTAATGAAAGGTGTTGAA
GGTACGGTTTCGAGTGTTATGTCACTTGTTGAACCTACTTCTATATACTCAGATGCTTTAGCGAAAGACACGGTTATTAA
TAAGGTTATTCCAGCTAGAAATATGCTCAAGTTGGAAGAGGTTTGA
ATGAAGTTGGTTTTAACCATAACAAGTTTTCACAAATTTACTCCAGAGATTGAGAGTGAGTTCGTGTTTGAATCAAGTGA
TGAACATGCCAAGGTTACATTTGGTAGGTCTGAACAATGTGATTGGACACTACCAGATCCTGAAAGAGTGGTATCCGGAA
CCCACGGAGAACTGATTAAGTTTGGTGACAAATACTTGATAAAAGACCTCTCAACTAATGGTACTTTTGTAAATAATGCT
GTAACTCCTGTGGGTCAAGGAAATGAAATAGCACTAAGCGATGACGATATGGTTAGCTTAGGGGATTATCAAATTTCAGT
TTCTATTCAAACAGATACAGATGTATTCAGTCTTGAGACAAGTAAAAGTCTTTCTAGCGCACCCCCTGAAGTAGTCTCAA
GTCCATCGGAAGCAGAATTTGTTGACCTAGGCCTTAGTGCTAGTGAACTCTTAAACGAAAGTGTTCCTTCTGACTTTCAG
CTAGATATTGGTTTAGTCGATGATTTTATTGATATCCCAAAGCCCGAAAAACAGAGCACCGTATCTGAAACTTTACCGAA
AGTTAACCGACAATCAGAACACCTATCGACTCCCAACTTTGATACCAATGGGGGTGAACTTGCGGCTTTTGTTAGAGGGG
TGGGTATAGATCCAATGATGATTCCCAGCGATAACCGAGAGGAATGGTTCGAACAACTGGGGGAGTCATTTTCTTTGATG
CTAGTTGGTTTAATGGAAACACTGCATAACAGAGCAGTGTTTAAACAAACAAATCGACTTAGTCATACCGCATTTCGTAA
GTCAGAAAATAACCCTTTAAAGTTTTCTGCAAACTTAGAAGATGCGATTCATAACTTATATAACCGTCAAACCGCTAGCT
TCTTAAAGCCAAATGCAGCCATTAAAGAAGCATTTAATGACATCGAAAGTCATGAGCAAGCCTTAATGAAAGGTGTTGAA
GGTACGGTTTCGAGTGTTATGTCACTTGTTGAACCTACTTCTATATACTCAGATGCTTTAGCGAAAGACACGGTTATTAA
TAAGGTTATTCCAGCTAGAAATATGCTCAAGTTGGAAGAGGTTTGA