Detailed information of component protein


Summary

Component ID T6CP010950
Component protein type TssI
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (311 a.a.); Nucleotide sequence (933 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0624
Coordinate (Strand) 27857..28789 (+)
NCBI ID 225382190
GenBank FJ790777
Uniprot ID C0LZR4_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 4e-64 30..308
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-310 MKRATQDNNFIRISSPFDKDAIILNSFEYREEISELFSLRAKAYFNDQRSELNEIIGKEVTITLENSEDVSSNPRYMHGYVSAARLEGKRVSNTHKGENYKNIELLIEPKLRFAEYRKNCRIFQHKDIKEIISEVLSEHSVAFSFELTKSYPKYTYKVQYEESDLEFVRRLLSEEGLSFCFTHTKSAHTLNIFDDISFYKPGTEFLVDFDTGTAKSSHISSWHETQILTTKASQKSGYNMLKPSSNPKNAAKGESDFFTVPGSEYFEYMGEMESNDQYALRNTHAIEALQQNAYLCRGEATCRTFSVNTH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9949 - -


  Protein sequence: 311 a.a.    

>T6CP010950 FJ790777:27857-28789 [Vibrio alginolyticus] [TssI]
MKRATQDNNFIRISSPFDKDAIILNSFEYREEISELFSLRAKAYFNDQRSELNEIIGKEVTITLENSEDVSSNPRYMHGY
VSAARLEGKRVSNTHKGENYKNIELLIEPKLRFAEYRKNCRIFQHKDIKEIISEVLSEHSVAFSFELTKSYPKYTYKVQY
EESDLEFVRRLLSEEGLSFCFTHTKSAHTLNIFDDISFYKPGTEFLVDFDTGTAKSSHISSWHETQILTTKASQKSGYNM
LKPSSNPKNAAKGESDFFTVPGSEYFEYMGEMESNDQYALRNTHAIEALQQNAYLCRGEATCRTFSVNTH*

  Nucleotide sequence: 933 bp    

>T6CP010950 FJ790777:27857-28789 [Vibrio alginolyticus] [TssI]
ATGAAAAGAGCAACTCAAGATAACAATTTCATCCGAATTTCTTCGCCATTCGATAAAGATGCCATCATACTAAACTCGTT
TGAGTATAGAGAAGAGATCTCAGAACTTTTTTCATTGCGTGCTAAAGCTTACTTTAATGATCAACGAAGTGAATTAAACG
AAATTATTGGTAAGGAGGTTACCATTACCCTTGAAAATAGTGAGGATGTTTCTTCAAATCCTCGTTATATGCATGGTTAT
GTTTCTGCTGCAAGATTAGAAGGGAAAAGGGTTTCTAATACACATAAAGGCGAAAATTATAAAAACATAGAATTATTAAT
AGAGCCTAAATTACGTTTCGCTGAATATCGAAAAAATTGCAGAATATTTCAGCATAAAGATATAAAGGAAATTATCTCTG
AAGTACTATCGGAGCATAGTGTTGCCTTTAGTTTTGAGTTAACCAAGTCCTATCCTAAATACACATACAAAGTACAATAT
GAAGAATCAGATCTTGAATTTGTTCGACGTCTGTTATCGGAGGAGGGGCTATCATTTTGTTTTACACATACTAAGTCTGC
CCATACGCTCAATATTTTTGATGACATCAGCTTTTATAAGCCGGGTACTGAGTTTTTAGTCGATTTTGATACAGGTACGG
CGAAATCTAGCCACATTTCTTCTTGGCATGAAACGCAAATTCTGACTACTAAAGCGAGTCAAAAGTCAGGCTATAACATG
CTCAAGCCTTCTTCGAATCCTAAAAATGCTGCAAAGGGTGAAAGTGATTTTTTTACAGTACCTGGAAGCGAGTATTTTGA
ATACATGGGTGAAATGGAGAGCAATGATCAATACGCACTGAGAAACACGCATGCAATAGAAGCACTACAGCAAAATGCAT
ATTTGTGTCGTGGCGAGGCTACGTGCAGAACATTCAGTGTCAATACGCACTGA