Detailed information of component protein


Summary

Component ID T6CP012878
Component protein type TagJ
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (265 a.a.); Nucleotide sequence (795 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0618
Coordinate (Strand) 20494..21288 (+)
NCBI ID 225382184
GenBank FJ790777
Uniprot ID B1PDM1_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpE PF07024.15 6.2e-35 138..257
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-264 MTQWKNALSQGLLQHALEQLIDEIKATPKDASLRSSFIELLCIDGDFERADAQLMQSIKLFPEYLPGASQLRHLVKAAQARKDFINGAASAKVLGDEELTKELVRFNLALGNQEYEEASRLSHRIEEHRTIKGFNVNGTNYEDARDIDDRLGGYIELFSTAGNYFLVPISSIHTLEVKAPTSLLESIWRPVEFDIDGLGEGEGHMPLTYVDSETDAQKLGRETDWKQIGEQESFVGLGQKCWLVDDSALPICDLTTLLEQKQIA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9880 - -


  Protein sequence: 265 a.a.    

>T6CP012878 FJ790777:20494-21288 [Vibrio alginolyticus] [TagJ]
MTQWKNALSQGLLQHALEQLIDEIKATPKDASLRSSFIELLCIDGDFERADAQLMQSIKLFPEYLPGASQLRHLVKAAQA
RKDFINGAASAKVLGDEELTKELVRFNLALGNQEYEEASRLSHRIEEHRTIKGFNVNGTNYEDARDIDDRLGGYIELFST
AGNYFLVPISSIHTLEVKAPTSLLESIWRPVEFDIDGLGEGEGHMPLTYVDSETDAQKLGRETDWKQIGEQESFVGLGQK
CWLVDDSALPICDLTTLLEQKQIA*

  Nucleotide sequence: 795 bp    

>T6CP012878 FJ790777:20494-21288 [Vibrio alginolyticus] [TagJ]
ATGACTCAATGGAAAAATGCTTTGTCCCAAGGCCTATTGCAACACGCTCTAGAGCAATTAATTGATGAAATTAAAGCAAC
ACCTAAAGATGCGAGCTTGAGAAGTTCCTTCATTGAGCTTCTGTGCATTGATGGTGACTTCGAGCGTGCGGACGCTCAGC
TCATGCAATCGATTAAATTGTTTCCTGAGTATCTGCCTGGTGCAAGTCAGCTACGACATTTAGTGAAAGCAGCTCAAGCT
AGAAAAGATTTTATCAATGGTGCGGCTAGTGCCAAAGTATTAGGTGATGAGGAATTAACTAAAGAGCTTGTTCGATTTAA
TCTTGCTCTCGGAAATCAAGAGTACGAAGAAGCGTCACGTCTATCGCATCGCATTGAAGAGCATCGTACTATTAAAGGGT
TTAACGTAAACGGCACAAACTACGAAGATGCTAGAGATATTGATGATCGTTTAGGTGGTTACATTGAACTGTTTTCTACA
GCAGGTAACTATTTTCTTGTACCCATATCGAGCATCCATACGCTAGAAGTTAAAGCACCTACTTCTTTGTTAGAGAGTAT
TTGGCGACCTGTCGAGTTTGATATCGATGGACTTGGTGAAGGCGAAGGCCATATGCCGTTGACATATGTTGACTCAGAGA
CCGATGCGCAAAAGTTAGGTCGAGAAACGGATTGGAAGCAGATTGGTGAGCAAGAATCTTTTGTTGGCTTGGGTCAAAAA
TGTTGGTTAGTCGATGACTCAGCGCTTCCAATCTGTGATTTGACTACATTATTAGAACAGAAACAAATAGCGTAG