Detailed information of regulator
Summary
| Regulator ID | REG0257  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | Biofilm formation - Pseudomonas aeruginosa (map02025) |
| Related T6SS | T6SS00794 (Type i5) |
| Strain | Vibrio alginolyticus strain EPJS |
| Replicon | chromosome |
| Sequence | Protein sequence (372 a.a.); Nucleotide sequence (1116 bp) |
| Description | Phosphorylation of PppA modulates the expression of Hcp2 by regulating LuxR |
| Reference |
External database links
| Locus tag (Gene) | VEPGS_0614 (PppA) |
| Coordinates (Strand) | 15765..16880 (+) |
| NCBI ID | 225382180 |
| GenBank | FJ790777 |
| Uniprot ID | C0LZQ4_VIBAL |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | map02025 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| LuxR | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ImpA_N | PF06812.14 | 2.5e-32 | 13..142 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-371 | MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTNQSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDDDGIETRISAIIGLNGIDSEGTLIFPIACIPLTDLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEAVGDVTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRESEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAEKSYFRLVGIAEQNEN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9876 | - | - |
Protein sequence of regulator: 372 a.a. .
>REG0257 FJ790777:15765-16880 [Vibrio alginolyticus]
MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTN
QSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDDDGIETRISAIIGLNGIDSEGTLIFPIACIPLT
DLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEAVGD
VTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRE
SEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAEKSYFRLVGIAEQNEN*
MSNEMFDWYEQALAPISQENPTGIDPREDVSPQSAYYRLKDQRMAARNAERNALIEEESIYTHSNLWRVFIEDVPEILTN
QSKDLEFVAWLIEALTRLYGFRGMGVGYKLATSLIENFWDDLYPMPDDDGIETRISAIIGLNGIDSEGTLIFPIACIPLT
DLGVEQAYAYWEYQQAIELERLDEDKRRNRVELGAVELSEIITTVKSTSDTFYQELITDLEFATESFNEFSQRLDEAVGD
VTPSSYISKRLESIHSALKHLLGDRFNQPQAVFEELEDLDESSSEQPLSSQSCDALLATNMKSREQAIRQLQQVADFFRE
SEPHSPVSYSIEQIIRWCGMPLPELLAELISDGDAEKSYFRLVGIAEQNEN*
Nucleotide sequence of regulator: 1116 bp .
>REG0257 FJ790777:15765-16880 [Vibrio alginolyticus]
ATGAGTAATGAAATGTTTGATTGGTATGAACAAGCGTTGGCTCCTATATCTCAAGAAAATCCTACAGGTATTGATCCTAG
AGAGGATGTATCACCACAGTCTGCTTACTATCGGTTGAAAGATCAGCGTATGGCCGCCCGCAACGCTGAACGTAATGCTC
TTATAGAAGAAGAAAGTATCTATACCCACAGTAATTTATGGCGAGTATTTATTGAAGATGTCCCAGAAATACTCACGAAT
CAGTCTAAAGATTTGGAATTTGTTGCCTGGCTTATAGAAGCCCTAACTCGATTGTATGGTTTTCGTGGTATGGGCGTTGG
CTACAAACTCGCAACCAGTTTAATTGAGAACTTTTGGGATGATCTGTACCCAATGCCTGATGATGATGGTATTGAGACAA
GAATATCGGCGATTATTGGCCTCAATGGTATCGATAGTGAAGGAACACTTATTTTTCCAATTGCTTGTATACCATTGACT
GACTTAGGCGTGGAGCAAGCTTATGCCTATTGGGAATATCAGCAAGCTATAGAGCTTGAGCGCTTAGACGAAGATAAGCG
CCGAAATCGAGTCGAGCTTGGGGCTGTTGAGCTATCTGAAATTATAACTACGGTTAAGTCGACTTCTGATACGTTTTATC
AAGAATTAATCACGGATTTGGAGTTCGCTACAGAATCATTTAATGAATTCAGTCAGCGATTAGACGAAGCGGTCGGTGAT
GTGACTCCTAGCTCTTATATATCAAAAAGACTCGAATCAATACACTCGGCATTAAAGCACCTTCTAGGAGATCGTTTCAA
TCAACCACAAGCCGTGTTTGAAGAGCTAGAGGATCTTGATGAATCTTCTTCTGAACAACCGTTAAGTTCACAAAGTTGTG
ATGCATTATTAGCGACAAACATGAAATCACGAGAGCAGGCAATTAGACAATTGCAGCAAGTAGCTGATTTCTTCCGAGAG
AGTGAACCTCACTCACCAGTTTCTTACTCCATCGAACAGATCATTCGTTGGTGTGGGATGCCTCTGCCGGAGTTACTGGC
GGAACTTATCTCCGACGGGGATGCAGAGAAAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATTAA
ATGAGTAATGAAATGTTTGATTGGTATGAACAAGCGTTGGCTCCTATATCTCAAGAAAATCCTACAGGTATTGATCCTAG
AGAGGATGTATCACCACAGTCTGCTTACTATCGGTTGAAAGATCAGCGTATGGCCGCCCGCAACGCTGAACGTAATGCTC
TTATAGAAGAAGAAAGTATCTATACCCACAGTAATTTATGGCGAGTATTTATTGAAGATGTCCCAGAAATACTCACGAAT
CAGTCTAAAGATTTGGAATTTGTTGCCTGGCTTATAGAAGCCCTAACTCGATTGTATGGTTTTCGTGGTATGGGCGTTGG
CTACAAACTCGCAACCAGTTTAATTGAGAACTTTTGGGATGATCTGTACCCAATGCCTGATGATGATGGTATTGAGACAA
GAATATCGGCGATTATTGGCCTCAATGGTATCGATAGTGAAGGAACACTTATTTTTCCAATTGCTTGTATACCATTGACT
GACTTAGGCGTGGAGCAAGCTTATGCCTATTGGGAATATCAGCAAGCTATAGAGCTTGAGCGCTTAGACGAAGATAAGCG
CCGAAATCGAGTCGAGCTTGGGGCTGTTGAGCTATCTGAAATTATAACTACGGTTAAGTCGACTTCTGATACGTTTTATC
AAGAATTAATCACGGATTTGGAGTTCGCTACAGAATCATTTAATGAATTCAGTCAGCGATTAGACGAAGCGGTCGGTGAT
GTGACTCCTAGCTCTTATATATCAAAAAGACTCGAATCAATACACTCGGCATTAAAGCACCTTCTAGGAGATCGTTTCAA
TCAACCACAAGCCGTGTTTGAAGAGCTAGAGGATCTTGATGAATCTTCTTCTGAACAACCGTTAAGTTCACAAAGTTGTG
ATGCATTATTAGCGACAAACATGAAATCACGAGAGCAGGCAATTAGACAATTGCAGCAAGTAGCTGATTTCTTCCGAGAG
AGTGAACCTCACTCACCAGTTTCTTACTCCATCGAACAGATCATTCGTTGGTGTGGGATGCCTCTGCCGGAGTTACTGGC
GGAACTTATCTCCGACGGGGATGCAGAGAAAAGCTACTTCCGTTTGGTTGGCATAGCAGAACAAAATGAAAATTAA