Detailed information of component protein


Summary

Component ID T6CP010946
Component protein type TssF
T6SS ID (Type) T6SS00794 experimental (Type i5)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (608 a.a.); Nucleotide sequence (1824 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
[3] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0620
Coordinate (Strand) 21757..23580 (+)
NCBI ID 225382186
GenBank FJ790777
Uniprot ID B1A3G8_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 1.1e-184 1..603
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-607 MSEEFLKYYNRELAYLRHKGQEFGEQYPKIAARLRISEEQVDDPHVERLLEGCAFLTARIRQSLDNSYPQFTESLMGQLYPDFHAPIPSMSVIKMSCSNSTSSYFDVEEGERVVIGATGYHECEFRTCYPVRMYPVDVTSGVFKNAPFKPVGSRWESNAHSVLKVQLTAQDEETPLGEMGIERLRFYLNGQSQLTFKLYQMLFQNLIGLSVVVDGKEVRPLTQRHLSEVGFSEDEKVVPYSKRSFSGARLLVEYLHFPEKFMFFDITELGLSELDLGEQVELYFYFDTNDDWLPKQVDEESLMVGCVPVINLFKSIMEPKRILPTEYDYHLSPQYQEAESNEVVSISDVTLRNWNKTYHNLPCYHAGEHTHYTNTSEIYWLMRREDKNWAGGYDEPGRETYISFVDKKHQLFSPDSRENWLMYVEAECCNRNLAQKLPFGGGLPQVSLPEVNDNFESVRCLVSLSETIRPEMDESTRWQLTKLLTLNHFTEEDGLATLKQTLNLYAFSGTAETKAVIDALTKLEFEPTTGRISQKGKVGFAHGIKIILTVSDQILPKEQIFLLGSVLSVYFSQYAEINIFTQLEIRLKSTGKSFHVWPALTGDKVLL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9953 - -


  Protein sequence: 608 a.a.    

>T6CP010946 FJ790777:21757-23580 [Vibrio alginolyticus] [TssF]
MSEEFLKYYNRELAYLRHKGQEFGEQYPKIAARLRISEEQVDDPHVERLLEGCAFLTARIRQSLDNSYPQFTESLMGQLY
PDFHAPIPSMSVIKMSCSNSTSSYFDVEEGERVVIGATGYHECEFRTCYPVRMYPVDVTSGVFKNAPFKPVGSRWESNAH
SVLKVQLTAQDEETPLGEMGIERLRFYLNGQSQLTFKLYQMLFQNLIGLSVVVDGKEVRPLTQRHLSEVGFSEDEKVVPY
SKRSFSGARLLVEYLHFPEKFMFFDITELGLSELDLGEQVELYFYFDTNDDWLPKQVDEESLMVGCVPVINLFKSIMEPK
RILPTEYDYHLSPQYQEAESNEVVSISDVTLRNWNKTYHNLPCYHAGEHTHYTNTSEIYWLMRREDKNWAGGYDEPGRET
YISFVDKKHQLFSPDSRENWLMYVEAECCNRNLAQKLPFGGGLPQVSLPEVNDNFESVRCLVSLSETIRPEMDESTRWQL
TKLLTLNHFTEEDGLATLKQTLNLYAFSGTAETKAVIDALTKLEFEPTTGRISQKGKVGFAHGIKIILTVSDQILPKEQI
FLLGSVLSVYFSQYAEINIFTQLEIRLKSTGKSFHVWPALTGDKVLL*

  Nucleotide sequence: 1824 bp    

>T6CP010946 FJ790777:21757-23580 [Vibrio alginolyticus] [TssF]
ATGAGTGAAGAGTTTCTCAAGTACTATAATCGCGAGTTAGCTTATTTGCGTCACAAAGGGCAAGAGTTTGGTGAGCAGTA
TCCCAAGATTGCCGCGAGACTTCGTATTAGTGAGGAACAGGTTGACGATCCACACGTAGAAAGATTGTTAGAAGGCTGCG
CATTTCTAACAGCAAGGATTAGACAAAGCTTAGACAATAGTTACCCTCAGTTTACAGAGTCTTTGATGGGACAGTTATAT
CCGGATTTTCACGCCCCAATCCCGTCAATGTCAGTCATTAAAATGAGCTGTTCTAACTCCACTTCCTCTTATTTCGATGT
AGAAGAAGGCGAGCGAGTTGTTATCGGTGCTACTGGATATCATGAATGCGAGTTTAGAACTTGTTATCCGGTCAGAATGT
ATCCTGTTGATGTGACCTCTGGCGTTTTTAAAAATGCGCCTTTCAAGCCAGTTGGCTCACGATGGGAAAGCAATGCTCAT
TCAGTTTTGAAAGTACAGCTCACGGCTCAAGATGAAGAGACTCCTCTTGGTGAAATGGGGATAGAACGGCTGCGCTTCTA
TTTAAACGGTCAATCTCAATTGACTTTTAAGCTCTACCAAATGCTGTTTCAAAACCTAATTGGTTTATCTGTTGTTGTGG
ATGGCAAAGAAGTTCGTCCTTTAACCCAAAGACATCTTTCTGAAGTAGGATTTTCAGAAGATGAGAAAGTTGTGCCATAC
AGTAAAAGAAGCTTTTCAGGAGCAAGATTACTTGTTGAGTATTTGCACTTTCCTGAGAAGTTTATGTTCTTTGATATCAC
CGAACTTGGATTATCTGAGCTAGATCTTGGAGAGCAAGTCGAGCTGTATTTTTACTTTGATACCAACGATGACTGGCTTC
CAAAGCAAGTGGACGAAGAAAGCTTAATGGTAGGTTGTGTGCCTGTAATCAACTTATTTAAAAGTATTATGGAGCCTAAG
CGTATTCTACCAACTGAATACGATTACCACTTGTCTCCACAATACCAAGAAGCTGAAAGTAATGAGGTTGTCTCAATTTC
TGACGTAACGTTGAGAAACTGGAACAAAACGTACCACAATTTGCCATGTTATCACGCTGGTGAGCACACGCATTATACGA
ACACTAGTGAGATTTATTGGTTGATGAGACGTGAGGATAAGAACTGGGCTGGTGGGTATGATGAGCCAGGTAGAGAAACT
TACATTTCTTTTGTTGATAAAAAACATCAATTGTTCAGCCCGGATAGCCGGGAAAACTGGTTAATGTACGTGGAAGCTGA
GTGCTGTAACCGCAACTTAGCGCAGAAACTTCCATTTGGAGGTGGGCTGCCGCAAGTATCTTTACCGGAGGTAAATGACA
ATTTCGAATCTGTTCGTTGTTTAGTTTCTTTATCAGAAACCATTCGGCCAGAAATGGATGAAAGCACACGATGGCAGTTA
ACTAAGCTTTTAACTTTAAACCATTTTACCGAGGAAGATGGTTTAGCAACTTTGAAGCAGACCCTTAATCTCTATGCATT
TTCTGGGACAGCAGAAACGAAAGCGGTAATCGATGCTTTAACTAAGTTAGAGTTTGAGCCTACTACAGGAAGAATTAGTC
AGAAAGGTAAAGTTGGATTTGCGCACGGTATAAAAATTATTTTGACGGTTTCTGATCAAATTTTACCTAAAGAGCAGATC
TTTCTCTTAGGTAGTGTTTTATCTGTTTACTTCTCGCAATATGCTGAAATTAACATTTTTACGCAGTTAGAAATAAGACT
TAAGTCCACGGGAAAGTCTTTCCACGTATGGCCCGCATTAACTGGCGATAAGGTGTTGCTATGA