Detailed information of regulator


Summary

Regulator ID REG0049 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (240 a.a.); Nucleotide sequence (720 bp)
Description Individual isogenic PAO1 mutants (ΔtagT, ΔtagS and ΔtagQ) were impaired in Hcp1 export,Co-production of TagS and TagT resulted in formation of a stable protein complex and The K/A mutation abolished ATPase activity of the TagTS complex show that the TagTS complex possesses ATPase activity that is dependent on the conserved Walker A motif within the TagT ATPase.TagT, TagS and TagQ act upstream of PpkA in the TPP.
Reference

External database links

Locus tag (Gene) PA0073 (TagT)
Coordinates (Strand) 86284..87003 (-)
NCBI ID 15595271
RefSeq NC_002516
Uniprot ID Q9I759_PSEAE
KEGG ID pae:PA0073
KEGG pathway (determined by BlastKOALA) -
PDB ID -

  Target
Target Regulation (↓/↑) Target sequence
PA0072 (TagS) 15595270

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
ABC_tran PF00005.29 1.1e-24 26..176

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-239 MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRDGRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIARALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEEVG



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9143 - -



  Protein sequence of regulator: 240 a.a.    .

>REG0049 NC_002516:c87003-86284 [Pseudomonas aeruginosa PAO1]
MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRD
GRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIAR
ALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEEVG*

  Nucleotide sequence of regulator: 720 bp    .

>REG0049 NC_002516:c87003-86284 [Pseudomonas aeruginosa PAO1]
ATGATCCTGCGCCTGCACGAGGTCGCCCGCAGCCACGGCGCCGGTCGCCAGCGCTACCGCCTGCGGGTGCCGGCGCTGGA
ATTGCGCGCGGGTCGCCAGCTGGCCATCGTCGGCCCCAGCGGCTGCGGCAAGAGCACCCTGCTCGACCTGCTGGCGCTGG
CCCTGGCGCCGGACCCGGGCGGGCGCTTCGAATTCCTCGCCGGGGAACGCCCGCAGGACATCGCCGCGCTCTGGCGCGAC
GGCCGCCAGGACCGCTTGGCGGCGCTGCGCCGGCAGCACTGCGGCTACGTGCTGCAAAGCGGCGGGTTGCTCGAGTTCCT
CGATGTGCGGCGCAACATCGGACTGCCCTGCGCGTTGCTCGGCGCCCCATCCGCCGAGCGCGTACAGGCGCTGGCCGAGC
GGCTGGAAATCGGCGACCAGCTGGACAAGAAACCGGCGGCGCTATCGGTCGGCCAGCGCCAGCGGGTCGCCATCGCCCGC
GCCCTGGCGCATGCGCCGCGCCTGTTGCTGGCCGACGAGCCGACCGCGGCGCTCGACCCGCTGAACGCCGAACGGGTGAT
GCGCCTGCTGGTGGAGCAGGCCCGCGAGCGCGGCGCCTGCACCGTGGTCGCCACCCACGACGAACGCCTGGCTCGCGCCG
CCGGCCTGGAAGTCCTGGCCATCCGCTGGCAACGCGAGGCCGACGACAGCGTCGGCGCCTGCCTCGAGGAAGTTGGCTGA