SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0049
Regulator type	protein
Regulatory pathway (from literatures)	Tpp
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00003 (Type i3)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (240 a.a.); Nucleotide sequence (720 bp)
Description	Individual isogenic PAO1 mutants (ΔtagT, ΔtagS and ΔtagQ) were impaired in Hcp1 export,Co-production of TagS and TagT resulted in formation of a stable protein complex and The K/A mutation abolished ATPase activity of the TagTS complex show that the TagTS complex possesses ATPase activity that is dependent on the conserved Walker A motif within the TagT ATPase.TagT, TagS and TagQ act upstream of PpkA in the TPP.
Reference	[1] Ho BT, Basler M, Mekalanos JJ. Type 6 secretion system-mediated immunity to type 4 secretion system-mediated gene transfer. Science. 2013 Oct 11;342(6155):250-3. doi: 10.1126/science.1243745. PMID: 24115441 [2] Casabona MG, Silverman JM, Sall KM, et al. An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environ Microbiol. 2013 Feb;15(2):471-86. doi: 10.1111/j.1462-2920.2012.02816.x. Epub 2012 Jul 6. PMID: 22765374

External database links

Locus tag (Gene)	PA0073 (TagT)
Coordinates (Strand)	86284..87003 (-)
NCBI ID	15595271
RefSeq	NC_002516
Uniprot ID	Q9I759_PSEAE
KEGG ID	pae:PA0073
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
PA0072 (TagS)	↓	15595270

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
ABC_tran	PF00005.29	1.1e-24	26..176

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-239	MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRDGRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIARALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEEVG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9143	-	-

Protein sequence of regulator: 240 a.a. .

                                    >REG0049 NC_002516:c87003-86284 [Pseudomonas aeruginosa PAO1]
MILRLHEVARSHGAGRQRYRLRVPALELRAGRQLAIVGPSGCGKSTLLDLLALALAPDPGGRFEFLAGERPQDIAALWRD
GRQDRLAALRRQHCGYVLQSGGLLEFLDVRRNIGLPCALLGAPSAERVQALAERLEIGDQLDKKPAALSVGQRQRVAIAR
ALAHAPRLLLADEPTAALDPLNAERVMRLLVEQARERGACTVVATHDERLARAAGLEVLAIRWQREADDSVGACLEEVG*

                                 

Nucleotide sequence of regulator: 720 bp .

                                    >REG0049 NC_002516:c87003-86284 [Pseudomonas aeruginosa PAO1]
ATGATCCTGCGCCTGCACGAGGTCGCCCGCAGCCACGGCGCCGGTCGCCAGCGCTACCGCCTGCGGGTGCCGGCGCTGGA
ATTGCGCGCGGGTCGCCAGCTGGCCATCGTCGGCCCCAGCGGCTGCGGCAAGAGCACCCTGCTCGACCTGCTGGCGCTGG
CCCTGGCGCCGGACCCGGGCGGGCGCTTCGAATTCCTCGCCGGGGAACGCCCGCAGGACATCGCCGCGCTCTGGCGCGAC
GGCCGCCAGGACCGCTTGGCGGCGCTGCGCCGGCAGCACTGCGGCTACGTGCTGCAAAGCGGCGGGTTGCTCGAGTTCCT
CGATGTGCGGCGCAACATCGGACTGCCCTGCGCGTTGCTCGGCGCCCCATCCGCCGAGCGCGTACAGGCGCTGGCCGAGC
GGCTGGAAATCGGCGACCAGCTGGACAAGAAACCGGCGGCGCTATCGGTCGGCCAGCGCCAGCGGGTCGCCATCGCCCGC
GCCCTGGCGCATGCGCCGCGCCTGTTGCTGGCCGACGAGCCGACCGCGGCGCTCGACCCGCTGAACGCCGAACGGGTGAT
GCGCCTGCTGGTGGAGCAGGCCCGCGAGCGCGGCGCCTGCACCGTGGTCGCCACCCACGACGAACGCCTGGCTCGCGCCG
CCGGCCTGGAAGTCCTGGCCATCCGCTGGCAACGCGAGGCCGACGACAGCGTCGGCGCCTGCCTCGAGGAAGTTGGCTGA

                                