Detailed information of regulator
Summary
| Regulator ID | REG0378  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Tpp |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00003 (Type i3) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (433 a.a.); Nucleotide sequence (1299 bp) |
| Description | A clear induction of H1-T6SS genes could be observed for an sg-tolB knockdown. |
| Reference |
External database links
| Locus tag (Gene) | PA0972 (tolB) |
| Coordinates (Strand) | 1056049..1057347 (+) |
| NCBI ID | NP_249663.1 |
| RefSeq | NC_002516 |
| Uniprot ID | TOLB_PSEAE |
| KEGG ID | pae:PA0972 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | 6S3W |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| ClpV-GFP foci accumulate; H1-T6SS transcript | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| TolB_N | PF04052.15 | 5.9e-29 | 25..126 |
| PD40 | PF07676.14 | 2.6e-13 | 279..314 |
| PD40 | PF07676.14 | 7.9e-13 | 236..271 |
| PD40 | PF07676.14 | 4.8e-07 | 197..227 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-432 | VSTLIRIALFALALMAGAAQAADPLVISSGNDRAIPIAVVPFGFQGGNVLPEDMSNIIGNDLRNSGYFEPLPRQNMISQPAQASEVIFRDWKAVGVNYVMVGNIVPAGGRLQVQYALFDVGTEQQVLTGSVTGSTDQLRDMSHYIADQSFEKLTGIKGAFSTKMLYVTAERFSVDNTRYTLQRSDYDGARPVTLLQSREPIVSPRFSPDGRRIAYVSFEQKRPRIFIQYVDTGRREQITNFEGLNGAPAFSPDGNRLAFVLSRDGNPEIYVMDLGSRALRRLTNNLAIDTEPFWGKDGSTLYFTSDRGGKPQIYKMNVNSGAVDRVTFIGNYNANPKLSADEKTLVMVHRQQGYTNFQIAAQDLQRGNLRVLSNTTLDDSPTVAPNGTMLIYATRQQDRGVLMLVSINGRVRIPLPTAQGDVREPSWSPYLN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9983 | pos: 21-22. AQA-AD. | VSTLIRIALFALALMAGAAQA |
Protein sequence of regulator: 433 a.a. .
>REG0378 NC_002516:1056049-1057347 [Pseudomonas aeruginosa PAO1]
VSTLIRIALFALALMAGAAQAADPLVISSGNDRAIPIAVVPFGFQGGNVLPEDMSNIIGNDLRNSGYFEPLPRQNMISQP
AQASEVIFRDWKAVGVNYVMVGNIVPAGGRLQVQYALFDVGTEQQVLTGSVTGSTDQLRDMSHYIADQSFEKLTGIKGAF
STKMLYVTAERFSVDNTRYTLQRSDYDGARPVTLLQSREPIVSPRFSPDGRRIAYVSFEQKRPRIFIQYVDTGRREQITN
FEGLNGAPAFSPDGNRLAFVLSRDGNPEIYVMDLGSRALRRLTNNLAIDTEPFWGKDGSTLYFTSDRGGKPQIYKMNVNS
GAVDRVTFIGNYNANPKLSADEKTLVMVHRQQGYTNFQIAAQDLQRGNLRVLSNTTLDDSPTVAPNGTMLIYATRQQDRG
VLMLVSINGRVRIPLPTAQGDVREPSWSPYLN*
VSTLIRIALFALALMAGAAQAADPLVISSGNDRAIPIAVVPFGFQGGNVLPEDMSNIIGNDLRNSGYFEPLPRQNMISQP
AQASEVIFRDWKAVGVNYVMVGNIVPAGGRLQVQYALFDVGTEQQVLTGSVTGSTDQLRDMSHYIADQSFEKLTGIKGAF
STKMLYVTAERFSVDNTRYTLQRSDYDGARPVTLLQSREPIVSPRFSPDGRRIAYVSFEQKRPRIFIQYVDTGRREQITN
FEGLNGAPAFSPDGNRLAFVLSRDGNPEIYVMDLGSRALRRLTNNLAIDTEPFWGKDGSTLYFTSDRGGKPQIYKMNVNS
GAVDRVTFIGNYNANPKLSADEKTLVMVHRQQGYTNFQIAAQDLQRGNLRVLSNTTLDDSPTVAPNGTMLIYATRQQDRG
VLMLVSINGRVRIPLPTAQGDVREPSWSPYLN*
Nucleotide sequence of regulator: 1299 bp .
>REG0378 NC_002516:1056049-1057347 [Pseudomonas aeruginosa PAO1]
GTGAGTACCCTGATTCGCATTGCGCTGTTCGCCCTGGCCCTGATGGCAGGTGCTGCGCAGGCTGCCGACCCGCTGGTGAT
CTCCAGCGGTAACGACCGTGCCATTCCCATCGCCGTGGTGCCGTTCGGCTTCCAGGGGGGCAACGTGTTGCCGGAAGACA
TGTCCAATATCATCGGCAACGACCTGCGCAACTCCGGCTACTTCGAGCCGCTGCCCCGGCAGAACATGATCAGCCAGCCG
GCGCAGGCCAGCGAAGTGATCTTCCGTGACTGGAAGGCAGTTGGCGTCAACTACGTGATGGTCGGCAACATCGTGCCTGC
CGGCGGACGCCTGCAGGTGCAGTACGCATTGTTCGATGTCGGCACCGAGCAGCAGGTCCTGACCGGCAGCGTGACCGGCA
GCACCGATCAGCTCCGCGACATGTCGCACTACATCGCCGACCAGTCGTTCGAGAAGCTCACCGGTATCAAGGGCGCGTTC
TCCACCAAGATGCTCTATGTCACCGCCGAGCGTTTCTCGGTCGACAACACCCGCTACACCTTGCAGCGCTCCGACTACGA
CGGCGCCCGCCCGGTGACCCTGCTGCAGTCGCGCGAGCCCATCGTGTCGCCGCGCTTCTCGCCCGATGGCCGCCGTATCG
CCTATGTCTCCTTCGAACAGAAGCGCCCGCGTATCTTCATCCAGTACGTCGATACTGGCCGTCGTGAGCAGATCACCAAT
TTCGAGGGCCTGAATGGCGCTCCGGCATTCTCCCCTGACGGCAACCGCCTGGCCTTCGTCCTGTCGCGCGACGGCAACCC
GGAAATCTACGTCATGGACCTTGGCAGCCGCGCCCTGCGCCGCCTGACCAACAACCTGGCGATCGATACCGAACCCTTCT
GGGGCAAGGACGGCTCTACCCTGTACTTCACCTCGGACCGTGGCGGCAAGCCGCAGATCTACAAAATGAACGTGAACAGC
GGTGCCGTGGACCGGGTAACCTTCATCGGCAACTACAACGCCAACCCGAAACTCTCGGCCGACGAGAAGACCCTGGTAAT
GGTCCACCGCCAGCAGGGCTACACCAACTTCCAGATCGCCGCCCAGGACCTCCAGCGCGGCAATCTGCGGGTGCTGTCGA
ACACCACTCTGGACGATTCGCCCACTGTTGCGCCCAATGGCACCATGCTAATCTACGCCACCCGCCAGCAGGACCGGGGC
GTGCTGATGCTCGTATCCATCAACGGACGCGTACGGATACCTCTCCCTACCGCTCAGGGCGATGTGCGCGAGCCTTCCTG
GTCCCCTTACCTGAACTGA
GTGAGTACCCTGATTCGCATTGCGCTGTTCGCCCTGGCCCTGATGGCAGGTGCTGCGCAGGCTGCCGACCCGCTGGTGAT
CTCCAGCGGTAACGACCGTGCCATTCCCATCGCCGTGGTGCCGTTCGGCTTCCAGGGGGGCAACGTGTTGCCGGAAGACA
TGTCCAATATCATCGGCAACGACCTGCGCAACTCCGGCTACTTCGAGCCGCTGCCCCGGCAGAACATGATCAGCCAGCCG
GCGCAGGCCAGCGAAGTGATCTTCCGTGACTGGAAGGCAGTTGGCGTCAACTACGTGATGGTCGGCAACATCGTGCCTGC
CGGCGGACGCCTGCAGGTGCAGTACGCATTGTTCGATGTCGGCACCGAGCAGCAGGTCCTGACCGGCAGCGTGACCGGCA
GCACCGATCAGCTCCGCGACATGTCGCACTACATCGCCGACCAGTCGTTCGAGAAGCTCACCGGTATCAAGGGCGCGTTC
TCCACCAAGATGCTCTATGTCACCGCCGAGCGTTTCTCGGTCGACAACACCCGCTACACCTTGCAGCGCTCCGACTACGA
CGGCGCCCGCCCGGTGACCCTGCTGCAGTCGCGCGAGCCCATCGTGTCGCCGCGCTTCTCGCCCGATGGCCGCCGTATCG
CCTATGTCTCCTTCGAACAGAAGCGCCCGCGTATCTTCATCCAGTACGTCGATACTGGCCGTCGTGAGCAGATCACCAAT
TTCGAGGGCCTGAATGGCGCTCCGGCATTCTCCCCTGACGGCAACCGCCTGGCCTTCGTCCTGTCGCGCGACGGCAACCC
GGAAATCTACGTCATGGACCTTGGCAGCCGCGCCCTGCGCCGCCTGACCAACAACCTGGCGATCGATACCGAACCCTTCT
GGGGCAAGGACGGCTCTACCCTGTACTTCACCTCGGACCGTGGCGGCAAGCCGCAGATCTACAAAATGAACGTGAACAGC
GGTGCCGTGGACCGGGTAACCTTCATCGGCAACTACAACGCCAACCCGAAACTCTCGGCCGACGAGAAGACCCTGGTAAT
GGTCCACCGCCAGCAGGGCTACACCAACTTCCAGATCGCCGCCCAGGACCTCCAGCGCGGCAATCTGCGGGTGCTGTCGA
ACACCACTCTGGACGATTCGCCCACTGTTGCGCCCAATGGCACCATGCTAATCTACGCCACCCGCCAGCAGGACCGGGGC
GTGCTGATGCTCGTATCCATCAACGGACGCGTACGGATACCTCTCCCTACCGCTCAGGGCGATGTGCGCGAGCCTTCCTG
GTCCCCTTACCTGAACTGA