Detailed information of regulator


Summary

Regulator ID REG0378 experimental
Regulator type protein
Regulatory pathway (from literatures) Tpp
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00003 experimental (Type i3)
Strain Pseudomonas aeruginosa PAO1
Replicon chromosome
Sequence Protein sequence (433 a.a.); Nucleotide sequence (1299 bp)
Description A clear induction of H1-T6SS genes could be observed for an sg-tolB knockdown.
Reference

External database links

Locus tag (Gene) PA0972 (tolB)
Coordinates (Strand) 1056049..1057347 (+)
NCBI ID NP_249663.1
RefSeq NC_002516
Uniprot ID TOLB_PSEAE
KEGG ID pae:PA0972
KEGG pathway (determined by BlastKOALA) -
PDB ID 6S3W

  Target
Target Regulation (↓/↑) Target sequence
ClpV-GFP foci accumulate; H1-T6SS transcript -

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
TolB_N PF04052.15 5.9e-29 25..126
PD40 PF07676.14 2.6e-13 279..314
PD40 PF07676.14 7.9e-13 236..271
PD40 PF07676.14 4.8e-07 197..227

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-432 VSTLIRIALFALALMAGAAQAADPLVISSGNDRAIPIAVVPFGFQGGNVLPEDMSNIIGNDLRNSGYFEPLPRQNMISQPAQASEVIFRDWKAVGVNYVMVGNIVPAGGRLQVQYALFDVGTEQQVLTGSVTGSTDQLRDMSHYIADQSFEKLTGIKGAFSTKMLYVTAERFSVDNTRYTLQRSDYDGARPVTLLQSREPIVSPRFSPDGRRIAYVSFEQKRPRIFIQYVDTGRREQITNFEGLNGAPAFSPDGNRLAFVLSRDGNPEIYVMDLGSRALRRLTNNLAIDTEPFWGKDGSTLYFTSDRGGKPQIYKMNVNSGAVDRVTFIGNYNANPKLSADEKTLVMVHRQQGYTNFQIAAQDLQRGNLRVLSNTTLDDSPTVAPNGTMLIYATRQQDRGVLMLVSINGRVRIPLPTAQGDVREPSWSPYLN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9983 pos: 21-22. AQA-AD. VSTLIRIALFALALMAGAAQA



  Protein sequence of regulator: 433 a.a.    .

>REG0378 NC_002516:1056049-1057347 [Pseudomonas aeruginosa PAO1]
VSTLIRIALFALALMAGAAQAADPLVISSGNDRAIPIAVVPFGFQGGNVLPEDMSNIIGNDLRNSGYFEPLPRQNMISQP
AQASEVIFRDWKAVGVNYVMVGNIVPAGGRLQVQYALFDVGTEQQVLTGSVTGSTDQLRDMSHYIADQSFEKLTGIKGAF
STKMLYVTAERFSVDNTRYTLQRSDYDGARPVTLLQSREPIVSPRFSPDGRRIAYVSFEQKRPRIFIQYVDTGRREQITN
FEGLNGAPAFSPDGNRLAFVLSRDGNPEIYVMDLGSRALRRLTNNLAIDTEPFWGKDGSTLYFTSDRGGKPQIYKMNVNS
GAVDRVTFIGNYNANPKLSADEKTLVMVHRQQGYTNFQIAAQDLQRGNLRVLSNTTLDDSPTVAPNGTMLIYATRQQDRG
VLMLVSINGRVRIPLPTAQGDVREPSWSPYLN*

  Nucleotide sequence of regulator: 1299 bp    .

>REG0378 NC_002516:1056049-1057347 [Pseudomonas aeruginosa PAO1]
GTGAGTACCCTGATTCGCATTGCGCTGTTCGCCCTGGCCCTGATGGCAGGTGCTGCGCAGGCTGCCGACCCGCTGGTGAT
CTCCAGCGGTAACGACCGTGCCATTCCCATCGCCGTGGTGCCGTTCGGCTTCCAGGGGGGCAACGTGTTGCCGGAAGACA
TGTCCAATATCATCGGCAACGACCTGCGCAACTCCGGCTACTTCGAGCCGCTGCCCCGGCAGAACATGATCAGCCAGCCG
GCGCAGGCCAGCGAAGTGATCTTCCGTGACTGGAAGGCAGTTGGCGTCAACTACGTGATGGTCGGCAACATCGTGCCTGC
CGGCGGACGCCTGCAGGTGCAGTACGCATTGTTCGATGTCGGCACCGAGCAGCAGGTCCTGACCGGCAGCGTGACCGGCA
GCACCGATCAGCTCCGCGACATGTCGCACTACATCGCCGACCAGTCGTTCGAGAAGCTCACCGGTATCAAGGGCGCGTTC
TCCACCAAGATGCTCTATGTCACCGCCGAGCGTTTCTCGGTCGACAACACCCGCTACACCTTGCAGCGCTCCGACTACGA
CGGCGCCCGCCCGGTGACCCTGCTGCAGTCGCGCGAGCCCATCGTGTCGCCGCGCTTCTCGCCCGATGGCCGCCGTATCG
CCTATGTCTCCTTCGAACAGAAGCGCCCGCGTATCTTCATCCAGTACGTCGATACTGGCCGTCGTGAGCAGATCACCAAT
TTCGAGGGCCTGAATGGCGCTCCGGCATTCTCCCCTGACGGCAACCGCCTGGCCTTCGTCCTGTCGCGCGACGGCAACCC
GGAAATCTACGTCATGGACCTTGGCAGCCGCGCCCTGCGCCGCCTGACCAACAACCTGGCGATCGATACCGAACCCTTCT
GGGGCAAGGACGGCTCTACCCTGTACTTCACCTCGGACCGTGGCGGCAAGCCGCAGATCTACAAAATGAACGTGAACAGC
GGTGCCGTGGACCGGGTAACCTTCATCGGCAACTACAACGCCAACCCGAAACTCTCGGCCGACGAGAAGACCCTGGTAAT
GGTCCACCGCCAGCAGGGCTACACCAACTTCCAGATCGCCGCCCAGGACCTCCAGCGCGGCAATCTGCGGGTGCTGTCGA
ACACCACTCTGGACGATTCGCCCACTGTTGCGCCCAATGGCACCATGCTAATCTACGCCACCCGCCAGCAGGACCGGGGC
GTGCTGATGCTCGTATCCATCAACGGACGCGTACGGATACCTCTCCCTACCGCTCAGGGCGATGTGCGCGAGCCTTCCTG
GTCCCCTTACCTGAACTGA