Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00793 experimental
T6SS type Type i1
Strain Vibrio alginolyticus EPJS
Replicon (GenBank) chromosome (FJ790776)
Location 1..32212
Function effector translocation
Comment In-frame deletion of VEPGS_0008 resulted in obvious biofilm reduction and the complemented strain restored to the level of the wild-type strain. Besides, in-frame deletion of dotU1, VEPGS_0008 and ppkA2 abolished the swarming ability. The interbacterial killing ability of V. alginolyticus EPGS is mediated by T6SS2.
References
[1] Sheng L et al (2012) Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 162(2-4):652-62. [PudMed:23021863]
[2] Liu H et al (2012) Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 55(1):73-81. [PudMed:22563695]
[3] Sheng L et al (2012) Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 194(5):379-90. [PudMed:22173829]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentFHATssATssBTssCTssDTssETssFTssGTssHTssITssKTssLTssM
Number1211111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
VEPGS_0001 75..1178 - 1104 225382144 hypothetical protein
T6CP010925 TssL VEPGS_0002 1679..2470 - 792 225382145 Dotu family type VI secretion protein
T6CP010926 TssK VEPGS_0003 2467..3792 - 1326 225382146 type VI secretion protein
T6CP012277 FHA VEPGS_0004 4297..5571 - 1275 225382147 type VI secretion system FHA-containing domain protein
VEPGS_0005 5800..6447 - 648 225382148 hypothetical protein
T6CP010927 TssA VEPGS_0006 6951..8264 + 1314 225382149 hypothetical protein
T6CP010928 TssM VEPGS_0007 8292..11681 + 3390 225382150 type VI secretion system IcmF
VEPGS_0008 11713..12165 + 453 225382151 transcriptional regulator
T6CP010929 TssG VEPGS_0009 12220..12741 - 522 225382152 hypothetical protein
T6CP010930 TssF VEPGS_0010 13544..15079 - 1536 225382153 hypothetical protein
T6CP010931 TssE VEPGS_0011 15090..15500 - 411 225382154 type VI secretion system lysozyme-related protein
T6CP010932 TssC VEPGS_0012 15562..17037 - 1476 225382155 hypothetical protein
T6CP010933 TssB VEPGS_0013 17046..17552 - 507 225382156 hypothetical protein
T6CP010934 TssA VEPGS_0014 17579..19243 - 1665 225382157 type VI secretion system-associated VCA0119
VEPGS_0015 19230..21230 - 2001 225382158 serine/threonine protein kinase
VEPGS_0016 21468..22412 - 945 225382159 hypothetical protein
VEPGS_0017 22405..22650 - 246 225382160 hypothetical protein
VEPGS_0018 23377..24696 - 1320 225382161 hypothetical protein
VEPGS_0019 24791..25189 - 399 225382162 hypothetical protein
T6CP010935 TssI VEPGS_0020 25276..27354 - 2079 225382163 putative VgrG protein
T6CP010936 TssD VEPGS_0021 27427..27945 - 519 225382164 uncharacterized hemolysin-coregulated protein
T6CP010937 TssH VEPGS_0022 28396..31080 + 2685 225382165 ATPases with chaperone activity ATP-binding subunit
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0261 experimental EPGS_00471 (VtsR) protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - LuxO (↑)
REG0263 experimental luxR protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - T6SS1 transcript and expression (↑)
REG0264 experimental VEPGS_0621 (pppA) protein Tpp; QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790777) 23577..24245 (+) 225382187 Unknown (↑); T6SS1 transcript (↑); hcp1 transcript (↓)
REG0265 experimental luxS protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - hcp1 transcript (↓)
REG0266 experimental LuxO protein QS regulatory pathway Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - hcp1 transcript (↓)
REG0268 experimental rpoN protein Transcription regulator Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - T6SS1 transcript (↓)
REG0269 experimental VasH protein Transcription regulator Vibrio alginolyticus strain EPJS chromosome (FJ790776) - - T6SS1 transcript (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01019 experimental VEPGS_0021 (hcp1) - - Vibrio alginolyticus strain EPJS chromosome (FJ790776) 27427..27945 (-) 225382164

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector