Detailed information of component protein


Summary

Component ID T6CP010934
Component protein type TssA
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (555 a.a.); Nucleotide sequence (1665 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0014
Coordinate (Strand) 17579..19243 (-)
NCBI ID 225382157
GenBank FJ790776
Uniprot ID C0LZN2_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 1.3e-70 277..527
ImpA_N PF06812.14 7.7e-27 10..120
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-554 MSNIDIDRLLNPISDESPVGNDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEEFGLQGFEQGLKLLSEALSRFGVELYPSRKRGRDGAVEWLNHQFKLVSSKLAKSAQSWDLVSNCISTIEEVQRQYDEVYQDSEADFFEIRTQLNVLSQQAAADEQSDDAVVNVEQAAPAPATQSAAEETPAADEQSDDAVVNVEQAAPAPATQPAAAETPAQSEVQTAPVKAVPPAPKPAPVKKAVAKEVDVDTDFSSPTASKRTLKKVAEVMLHANPSDPLAYRIYRHLTWDDIDGLPDHQNNQTPLSLAVSSDQQAEYRDKADQESDIDTIKRLERTLTDAPFWLTGHYFVYSMLNNLGFDDAALAVKQEVTRFVESLEGIEHLSFKNSIPFADEATLSWLSTQDTTSSTSQPVVQTVVISQEDSLPMEDITLENLGECAAELAHKLELDSSGRGQFMLYLQLIAAYQSVGLYPLCLPYLEKGWEVQKAFNLASWEPHLSSQLEDLIRKTLHRLFGSKDLLPEKYEEWKAIYD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9962 - -


  Protein sequence: 555 a.a.    

>T6CP010934 FJ790776:c19243-17579 [Vibrio alginolyticus] [TssA]
MSNIDIDRLLNPISDESPVGNDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEE
FGLQGFEQGLKLLSEALSRFGVELYPSRKRGRDGAVEWLNHQFKLVSSKLAKSAQSWDLVSNCISTIEEVQRQYDEVYQD
SEADFFEIRTQLNVLSQQAAADEQSDDAVVNVEQAAPAPATQSAAEETPAADEQSDDAVVNVEQAAPAPATQPAAAETPA
QSEVQTAPVKAVPPAPKPAPVKKAVAKEVDVDTDFSSPTASKRTLKKVAEVMLHANPSDPLAYRIYRHLTWDDIDGLPDH
QNNQTPLSLAVSSDQQAEYRDKADQESDIDTIKRLERTLTDAPFWLTGHYFVYSMLNNLGFDDAALAVKQEVTRFVESLE
GIEHLSFKNSIPFADEATLSWLSTQDTTSSTSQPVVQTVVISQEDSLPMEDITLENLGECAAELAHKLELDSSGRGQFML
YLQLIAAYQSVGLYPLCLPYLEKGWEVQKAFNLASWEPHLSSQLEDLIRKTLHRLFGSKDLLPEKYEEWKAIYD*

  Nucleotide sequence: 1665 bp    

>T6CP010934 FJ790776:c19243-17579 [Vibrio alginolyticus] [TssA]
TTGTCCAACATTGATATCGATCGTCTGCTTAATCCTATTTCTGATGAATCTCCAGTGGGAAACGATGCCCGTTACGAGTT
TTGCTATGAAATGATGGAGTCCGAAGTGAAGAAATTCGGTTCCTTGTTTGGCGAAACAGTAGATTGGAGCGTGGTAAAAA
CGCACGCGACTGAAGTTCTAGAGCACCACAGTAAAGACTTAAAAGCCATTTGTTACCTCGTTCGCGCGCTAACAGAAGAG
TTTGGTCTGCAAGGCTTTGAACAAGGTCTAAAGCTGCTTAGTGAGGCGTTAAGTCGCTTTGGCGTTGAATTGTATCCTTC
GCGAAAGCGGGGTCGAGATGGCGCGGTCGAATGGTTGAACCATCAGTTTAAACTGGTCAGCAGCAAGTTAGCGAAAAGCG
CTCAGTCATGGGATTTGGTATCAAACTGCATTTCGACTATTGAAGAAGTGCAGCGCCAGTACGACGAGGTTTACCAAGAC
TCAGAAGCGGATTTCTTTGAGATTCGCACTCAACTCAATGTTTTATCTCAACAAGCGGCGGCTGATGAGCAGAGCGATGA
TGCAGTCGTTAACGTCGAACAAGCGGCGCCTGCACCAGCTACGCAGTCTGCTGCCGAAGAAACGCCAGCGGCTGATGAGC
AGAGCGATGATGCAGTCGTTAACGTCGAACAAGCGGCGCCTGCACCAGCTACGCAGCCTGCTGCCGCAGAAACGCCAGCG
CAGAGTGAAGTGCAAACCGCACCGGTGAAGGCGGTTCCGCCGGCCCCTAAACCTGCGCCAGTTAAGAAAGCGGTAGCAAA
AGAAGTCGATGTCGATACGGACTTCTCTTCGCCGACGGCTTCTAAGCGCACCTTGAAGAAGGTTGCGGAAGTCATGCTTC
ATGCGAACCCAAGTGATCCGCTGGCTTACCGGATTTATCGTCATCTGACTTGGGATGACATTGATGGTCTACCCGACCAC
CAAAATAATCAAACGCCTTTGAGCTTGGCAGTGTCGTCCGATCAACAAGCGGAATACCGAGATAAAGCTGACCAAGAAAG
CGACATCGACACGATTAAGCGTTTGGAGCGCACATTAACGGATGCGCCGTTTTGGCTGACGGGCCATTACTTCGTGTATT
CGATGCTTAATAACTTGGGCTTTGATGATGCAGCGCTTGCTGTGAAACAAGAAGTGACGCGTTTTGTTGAATCGCTAGAG
GGCATCGAGCACCTCTCATTTAAGAATTCTATACCATTTGCAGATGAAGCGACCTTGAGCTGGTTATCGACTCAAGATAC
TACTTCTTCGACTTCTCAACCCGTTGTTCAAACGGTTGTGATTTCGCAGGAAGACTCGCTGCCAATGGAGGATATCACTC
TAGAAAATCTAGGGGAATGTGCCGCAGAGCTTGCCCACAAACTTGAGCTGGATAGCTCGGGACGTGGGCAGTTCATGCTG
TATTTACAATTGATAGCAGCTTACCAGTCTGTTGGATTATACCCGTTGTGTTTGCCGTATCTTGAGAAGGGGTGGGAAGT
ACAAAAAGCGTTTAATCTCGCGAGCTGGGAACCCCACTTATCGTCACAGTTAGAAGACCTAATTCGAAAAACACTCCATC
GGTTATTTGGAAGCAAGGATCTTCTACCTGAGAAGTATGAAGAGTGGAAAGCAATTTACGACTAA