Detailed information of component protein


Summary

Component ID T6CP010928
Component protein type TssM
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0007
Coordinate (Strand) 8292..11681 (+)
NCBI ID 225382150
GenBank FJ790776
Uniprot ID C0LZM5_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 5.5e-48 154..408
IcmF-related PF06761.14 7.7e-43 485..755
IcmF_C PF06744.14 5.4e-18 1004..1107
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MFKQKL
Transmembrane helix 7-29 FRNPVVISTILMFLVVAIITTIY
Outside 30-38 LLFLDEIDQ
Transmembrane helix 39-61 LYLWLTLGAIAMVYVTFQFTLWL
Inside 62-401 KKKNNAKAQHLETEEEALSMVIQPLLANSGKKPIYLMLGNKGSGRRQFLFNSSAIKPMDRTRTAKNDFFEWYESDSAVYIKPDQRLVFQEVSSSDASLWNTFIEEVIHHRPRKPFSGCLFFTDFEFMIVSEPEQKEYTISSLLARLSTINSKTSSALPIYLIMSKLDKLDGFKEYMHFSSLKTRVEFLSIPLKEAKGVFAEYYRDSYRNLVRVLESNALDASANSTDIDEKQAILAFPKQFELCQSEVSYVLERLCDVNNGVYSLDIREIFFSSSLQGGRKYNLLAKSCSNYFSLPIIASEHTQLTETPYFSRFLIDSQILPESDFAGENKTYLRLIQRQ
Transmembrane helix 402-424 SRLAILGSIILLTGGSYFFFTTL
Outside 425-1129 DSNLHVMNQLLSIDDKSQVEQDARFDELLAKATKAIEPSYGAWLKGSKALDEEILPLNISRLDQSTRIAYEALLKDVAQRLIPLIEKGCRLQLTQNQNTFTRALPLLKGYLMLNDPTKRDIRFLRHQTLQMMNDLSNQNDVVNQAMRYLDAYFRTQFDPIDINMDLVRATRRSLLANSNVDLVYAGILNEANSIDLGTLDLQRAVGFEFSNVFNTPIDSERLIVDKIYTSTGFSTFYRPRVDLMSQQVISDNWVLGLSQHVIPTSEEQEAFKEEVRKKYTDDYISNWRNALSELKVQNYDNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNALISKLNPKLVAAADSASDAVEEVVKPDYLLMKRVEQAFHLLNQLQVSETPNSPTPWDETVTALSRVRTYMKDIADAPDPQMAALAAAQHRMNSSEADPLIKLKQIAQKSPEPVRSWLLDVVQQSWSVMIKESAKGIQTQWYSEIYTKFKEIGLGRYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTNLWTPKQVDGETMPISPALLVQLRNYNVIRDTLINKSTNRVHIPFSARVLDLDSSAIRASLKIADTNISYYHGPSRIREMAWPPQNGDFNISITLQDVTDEGRQHVLNKSGQWAIYKLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAELYNFTLPESI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9987 - -


  Protein sequence: 1130 a.a.    

>T6CP010928 FJ790776:8292-11681 [Vibrio alginolyticus] [TssM]
MFKQKLFRNPVVISTILMFLVVAIITTIYLLFLDEIDQLYLWLTLGAIAMVYVTFQFTLWLKKKNNAKAQHLETEEEALS
MVIQPLLANSGKKPIYLMLGNKGSGRRQFLFNSSAIKPMDRTRTAKNDFFEWYESDSAVYIKPDQRLVFQEVSSSDASLW
NTFIEEVIHHRPRKPFSGCLFFTDFEFMIVSEPEQKEYTISSLLARLSTINSKTSSALPIYLIMSKLDKLDGFKEYMHFS
SLKTRVEFLSIPLKEAKGVFAEYYRDSYRNLVRVLESNALDASANSTDIDEKQAILAFPKQFELCQSEVSYVLERLCDVN
NGVYSLDIREIFFSSSLQGGRKYNLLAKSCSNYFSLPIIASEHTQLTETPYFSRFLIDSQILPESDFAGENKTYLRLIQR
QSRLAILGSIILLTGGSYFFFTTLDSNLHVMNQLLSIDDKSQVEQDARFDELLAKATKAIEPSYGAWLKGSKALDEEILP
LNISRLDQSTRIAYEALLKDVAQRLIPLIEKGCRLQLTQNQNTFTRALPLLKGYLMLNDPTKRDIRFLRHQTLQMMNDLS
NQNDVVNQAMRYLDAYFRTQFDPIDINMDLVRATRRSLLANSNVDLVYAGILNEANSIDLGTLDLQRAVGFEFSNVFNTP
IDSERLIVDKIYTSTGFSTFYRPRVDLMSQQVISDNWVLGLSQHVIPTSEEQEAFKEEVRKKYTDDYISNWRNALSELKV
QNYDNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNALISKLNPKLVAAADSASDAVEEVVKPDYLLMKRVEQ
AFHLLNQLQVSETPNSPTPWDETVTALSRVRTYMKDIADAPDPQMAALAAAQHRMNSSEADPLIKLKQIAQKSPEPVRSW
LLDVVQQSWSVMIKESAKGIQTQWYSEIYTKFKEIGLGRYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTN
LWTPKQVDGETMPISPALLVQLRNYNVIRDTLINKSTNRVHIPFSARVLDLDSSAIRASLKIADTNISYYHGPSRIREMA
WPPQNGDFNISITLQDVTDEGRQHVLNKSGQWAIYKLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAEL
YNFTLPESI*

  Nucleotide sequence: 3390 bp    

>T6CP010928 FJ790776:8292-11681 [Vibrio alginolyticus] [TssM]
ATGTTCAAACAAAAACTGTTTAGAAATCCAGTGGTGATTTCTACTATATTGATGTTTTTAGTGGTTGCAATTATCACCAC
TATTTATCTGCTTTTTCTTGACGAAATCGATCAACTTTATCTTTGGCTGACCCTTGGTGCTATCGCCATGGTTTACGTCA
CTTTTCAATTCACATTATGGTTGAAGAAAAAGAACAACGCTAAAGCTCAACATTTGGAAACCGAGGAAGAAGCACTTTCT
ATGGTTATCCAGCCTCTTCTTGCTAACTCAGGCAAGAAACCAATTTACCTGATGCTTGGTAATAAAGGGTCAGGGCGACG
TCAATTTTTATTTAATTCTAGTGCAATTAAACCGATGGATCGCACTAGAACCGCTAAGAATGACTTTTTTGAATGGTACG
AATCTGACAGCGCTGTGTACATCAAACCAGACCAACGCTTAGTGTTCCAGGAAGTATCAAGCAGTGATGCTTCTTTATGG
AATACGTTCATTGAAGAAGTTATTCATCATCGACCTCGAAAGCCATTTTCTGGGTGTCTATTCTTCACCGATTTCGAGTT
CATGATCGTCTCTGAGCCAGAGCAAAAAGAGTACACCATTTCGTCGTTATTGGCGCGCTTGAGTACCATCAACTCTAAAA
CGTCTTCAGCATTGCCAATCTATTTGATTATGTCAAAGCTAGACAAGTTAGACGGCTTTAAAGAATACATGCACTTTAGC
TCTCTCAAGACACGTGTTGAGTTCCTCTCTATCCCACTAAAAGAAGCCAAAGGTGTATTTGCAGAGTACTACCGCGATAG
CTATCGAAATCTAGTGAGAGTGCTTGAAAGTAATGCGCTAGATGCATCCGCTAATTCAACGGACATTGATGAGAAACAAG
CAATACTCGCTTTCCCTAAGCAGTTTGAGCTATGTCAGTCTGAAGTCAGTTATGTGCTTGAGCGTCTATGTGATGTTAAC
AACGGTGTCTATTCACTAGACATCCGTGAGATTTTCTTTAGTTCGAGTCTGCAAGGCGGTCGCAAGTACAATTTACTTGC
AAAAAGTTGTAGTAATTACTTCAGCTTACCTATTATCGCGTCAGAGCATACTCAGTTAACAGAAACGCCTTATTTCTCTC
GCTTTCTGATTGATTCACAAATATTGCCGGAAAGCGACTTTGCGGGCGAAAACAAGACGTATCTTCGTCTAATTCAGAGA
CAAAGCCGTTTGGCAATACTCGGCTCTATTATCCTGCTTACTGGGGGCAGTTACTTCTTTTTCACGACATTAGACAGTAA
CTTACATGTGATGAATCAACTCTTGAGTATCGATGACAAATCACAAGTGGAGCAAGATGCAAGATTCGATGAGCTTTTAG
CGAAGGCGACGAAAGCAATAGAGCCTTCTTATGGGGCGTGGCTTAAAGGTTCTAAGGCTTTGGATGAAGAGATATTACCA
CTCAACATTAGCCGTTTGGATCAAAGCACTCGCATCGCTTACGAAGCATTACTTAAGGACGTTGCTCAACGGTTGATACC
TCTCATTGAGAAAGGTTGCCGTCTACAACTGACACAAAATCAAAACACTTTTACACGCGCTTTACCTCTATTGAAGGGGT
ATCTAATGCTAAACGATCCAACAAAGCGTGATATCCGTTTTTTGCGTCATCAAACTCTCCAAATGATGAACGACTTGAGC
AACCAAAATGACGTCGTCAATCAGGCGATGCGCTATTTGGACGCTTACTTTCGCACACAGTTTGATCCAATTGATATCAA
TATGGATCTTGTTCGCGCAACACGTCGCTCATTGCTAGCAAATTCGAATGTTGATCTCGTTTATGCAGGCATTTTAAACG
AAGCAAATTCGATTGATTTAGGAACCCTAGATCTGCAACGAGCTGTGGGTTTTGAGTTTAGCAATGTGTTCAACACACCA
ATCGATAGCGAACGCTTGATCGTAGATAAGATCTATACCTCCACTGGTTTTAGCACTTTTTATCGACCACGCGTGGACCT
AATGTCACAACAAGTCATCAGTGACAACTGGGTTTTAGGTCTGTCACAACACGTTATTCCTACTAGTGAAGAACAAGAAG
CCTTCAAAGAGGAAGTACGTAAAAAATATACTGACGACTACATCAGTAATTGGCGCAATGCGCTGAGTGAACTAAAAGTC
CAGAACTATGACAATGTCGGCGATCTAACGAACGCGATAGACTTAATCTCGGGCCCTTCTTCACCAATGACAACCGTACT
GAAGCAGGTCTATGCCAACACACAATTTTCACCCGTTGGTGAAAAAAATGCTCTGATTTCAAAGCTAAACCCGAAACTCG
TGGCAGCGGCAGACTCCGCATCAGACGCAGTAGAGGAAGTAGTTAAGCCTGATTACTTGTTGATGAAACGTGTTGAACAA
GCTTTTCACTTATTAAACCAACTTCAGGTCAGTGAAACACCAAACTCCCCTACTCCTTGGGATGAGACCGTAACAGCGCT
AAGCCGTGTTCGCACGTATATGAAAGACATTGCTGATGCACCTGACCCGCAAATGGCGGCGCTAGCGGCAGCTCAGCATC
GAATGAACAGCAGTGAAGCTGACCCATTAATAAAACTTAAACAGATTGCCCAAAAATCACCGGAACCCGTTCGTAGCTGG
TTGTTAGATGTGGTTCAACAAAGCTGGTCAGTCATGATTAAAGAGTCCGCTAAAGGCATTCAAACCCAATGGTACAGCGA
GATTTACACCAAGTTTAAAGAGATAGGGCTAGGACGATACCCATTTGATCTAACAGCGACCGAGGAAATCTCAATTGAAG
ATTTTGAATTACTGTTTGCTTCAGGTGGCTTGTTAGACACATTTATTCAGAAAAATTTTGCACCGTTCTACGATACCAAT
TTGTGGACGCCAAAACAGGTGGATGGCGAGACGATGCCAATCTCTCCGGCTCTGTTAGTGCAACTGCGTAACTATAACGT
TATTCGAGACACATTAATTAATAAAAGCACTAACCGCGTTCATATTCCGTTTAGTGCAAGAGTACTTGATCTTGATTCTA
GTGCGATTAGAGCGAGCTTAAAAATTGCAGATACCAATATAAGTTACTACCACGGTCCAAGCCGCATTCGTGAAATGGCA
TGGCCACCGCAAAATGGTGACTTCAATATTAGTATCACACTCCAGGATGTAACCGATGAAGGCAGACAGCACGTTTTAAA
TAAGAGTGGTCAATGGGCGATATACAAATTGCTAGGTGACTCGACCTTGACAAACACTCATAACGGCAGCTTTGTCAGTG
ACATAAAAGTGTCAGGGAGGGACTTGAGCTTACGCATCACTCCGTTGACACAGAAAAACCCATTCACTCTTGCAGAGCTG
TATAACTTTACGCTCCCTGAGTCGATTTAA