SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01019
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00793 (Type i1)
Strain	Vibrio alginolyticus strain EPJS
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Description	-
Reference	[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863 [2] Yang Z, Zhou X, Ma Y, et al. Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus. Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19. PMID: 29314504

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VEPGS_0021 (hcp1)
Coordinates (Strand)	27427..27945 (-)
NCBI ID	225382164
GenBank	FJ790776
Uniprot ID	B1PDM0_VIBAL
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	6.4e-31	6..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MPTPAYMSIQGETQGHITKDTYSADSVGNTWQEAHVDEFLVQELDHVLTVPRDPQSGQPTGQRVHRPLVVTKVQDRSSPLLFNALVSGEKLPECVIRFYRTSVQGKQEHYYSIKLIDALLVDIQTRMNHCQDAATADRVTEEVLKFTYRAIEVTHENCGTAGNDDWRAPREA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9660	-	-

Protein sequence: 173 a.a. .

                                    >EFF01019 FJ790776:c27945-27427  (Unknown) [Vibrio alginolyticus]
MPTPAYMSIQGETQGHITKDTYSADSVGNTWQEAHVDEFLVQELDHVLTVPRDPQSGQPTGQRVHRPLVVTKVQDRSSPL
LFNALVSGEKLPECVIRFYRTSVQGKQEHYYSIKLIDALLVDIQTRMNHCQDAATADRVTEEVLKFTYRAIEVTHENCGT
AGNDDWRAPREA*

                                

Nucleotide sequence: 519 bp .

                                    >EFF01019 FJ790776:c27945-27427  (Unknown) [Vibrio alginolyticus]
ATGCCAACTCCAGCATATATGTCTATCCAAGGTGAGACTCAAGGCCATATTACTAAAGATACTTACTCTGCAGATTCAGT
AGGTAACACATGGCAAGAAGCTCACGTTGATGAGTTCCTAGTACAAGAACTTGATCACGTGCTAACTGTTCCACGTGATC
CACAAAGTGGTCAGCCAACTGGTCAACGTGTACACCGTCCACTTGTTGTAACTAAAGTTCAAGATCGCTCATCTCCACTA
CTATTCAACGCACTAGTATCTGGCGAAAAACTTCCTGAGTGTGTGATCCGCTTCTACCGTACTTCAGTTCAAGGTAAACA
AGAGCACTACTACTCAATCAAATTGATTGATGCGCTACTTGTCGATATCCAAACTCGTATGAACCACTGTCAAGACGCAG
CAACAGCTGACCGCGTGACAGAAGAAGTGCTTAAGTTCACTTACCGTGCGATCGAAGTAACTCACGAAAACTGTGGTACT
GCGGGTAACGACGACTGGCGTGCTCCACGCGAAGCTTAA

                                