Detailed information of component protein


Summary

Component ID T6CP010935
Component protein type TssI
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (693 a.a.); Nucleotide sequence (2079 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0020
Coordinate (Strand) 25276..27354 (-)
NCBI ID 225382163
GenBank FJ790776
Uniprot ID C0LZN8_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 6.5e-78 26..331
Phage_base_V PF04717.14 2.9e-15 387..455
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-692 MVNDAEFKFEVPGCGHEFRVESFHVHEELSKPFHINLSLLSLDPDISFDELIRKAGTLTLYGQGVGAARVFNGVVNEVRYLGTGRRFSRYQLVLVPQAWFLSQRQDCRIFQQKSAQDIITEVLDDGAVTDYRFEVSGTYPPKEYVLQYRESDLHFVQRMMAEHGMWYYFDHSDSNHTMVIVDSNDAIAPLISSPLNASYIGPIVYHADGGGVADREHISDLELVNRVRTGQVTYTDYNYEHPKIPQEMTQAGELDQDLKQFDYPGRYVDPLMGQVRTTEWMSEHIVDNQQVEATSDAMRLASGYSFNVSDHPRSEINRDYLMLSVMHTGQDPQVHEDEASGMPTTYYNQFSCIPRDVVFKAPKLAAPVVDGPQTAVVVGPEGEEIYTDKLGRVKVQFHWDRYGDNNEHSSCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHFPPYSLPENKTRTTFRTQTHKGTGYNELSFEDEANQEEVYIHAQKDMSTKVLNNRYRDIGQDEFLKVARHQTNEVHGDHKETIHGHKTTQVNSTFTETVEQDVTVTYNANEAQYVKNNSDLEIGDNRTTKIGKNDDLDIGENSNLTIGASKSSDIGADDNQTIGGNLTVSVKGNTAYKADGATQIISGDKIVLKTGGSSLVMNSDGSIKLSGSAITIEGSDKVVVKGGNVVVN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9806 - -


  Protein sequence: 693 a.a.    

>T6CP010935 FJ790776:c27354-25276 [Vibrio alginolyticus] [TssI]
MVNDAEFKFEVPGCGHEFRVESFHVHEELSKPFHINLSLLSLDPDISFDELIRKAGTLTLYGQGVGAARVFNGVVNEVRY
LGTGRRFSRYQLVLVPQAWFLSQRQDCRIFQQKSAQDIITEVLDDGAVTDYRFEVSGTYPPKEYVLQYRESDLHFVQRMM
AEHGMWYYFDHSDSNHTMVIVDSNDAIAPLISSPLNASYIGPIVYHADGGGVADREHISDLELVNRVRTGQVTYTDYNYE
HPKIPQEMTQAGELDQDLKQFDYPGRYVDPLMGQVRTTEWMSEHIVDNQQVEATSDAMRLASGYSFNVSDHPRSEINRDY
LMLSVMHTGQDPQVHEDEASGMPTTYYNQFSCIPRDVVFKAPKLAAPVVDGPQTAVVVGPEGEEIYTDKLGRVKVQFHWD
RYGDNNEHSSCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHFPPYSLPENKTRTTFRTQTHK
GTGYNELSFEDEANQEEVYIHAQKDMSTKVLNNRYRDIGQDEFLKVARHQTNEVHGDHKETIHGHKTTQVNSTFTETVEQ
DVTVTYNANEAQYVKNNSDLEIGDNRTTKIGKNDDLDIGENSNLTIGASKSSDIGADDNQTIGGNLTVSVKGNTAYKADG
ATQIISGDKIVLKTGGSSLVMNSDGSIKLSGSAITIEGSDKVVVKGGNVVVN*

  Nucleotide sequence: 2079 bp    

>T6CP010935 FJ790776:c27354-25276 [Vibrio alginolyticus] [TssI]
ATGGTGAATGACGCAGAATTTAAATTTGAAGTGCCTGGATGTGGGCATGAATTTCGGGTTGAGAGCTTTCATGTTCATGA
AGAGTTATCTAAGCCTTTCCACATCAATCTCTCATTACTTTCACTTGATCCAGATATCTCGTTCGATGAGTTAATACGTA
AAGCAGGAACATTGACACTTTACGGACAAGGTGTTGGGGCTGCGCGTGTGTTCAACGGCGTGGTGAATGAAGTTCGTTAC
TTAGGGACTGGACGTCGTTTTTCTCGTTATCAATTAGTCTTGGTTCCTCAAGCTTGGTTCTTATCTCAGCGCCAAGATTG
TCGTATTTTCCAGCAAAAGTCAGCGCAAGACATCATCACAGAAGTACTCGATGATGGGGCAGTCACCGATTACCGATTTG
AAGTGTCGGGTACTTATCCCCCAAAAGAATACGTTCTGCAATACCGTGAGTCTGACTTGCACTTCGTGCAGCGTATGATG
GCCGAACACGGTATGTGGTATTACTTTGACCATAGCGACTCAAATCATACGATGGTCATTGTCGACAGTAATGACGCGAT
TGCACCTTTGATAAGCTCGCCACTAAATGCTTCTTACATTGGGCCGATTGTTTATCATGCCGATGGTGGCGGTGTTGCCG
ATCGCGAACATATCTCTGATCTCGAGTTAGTTAACCGCGTTAGAACGGGACAGGTTACGTACACAGATTACAACTACGAA
CACCCTAAAATTCCACAAGAGATGACACAGGCTGGCGAGCTAGACCAAGATCTCAAGCAGTTTGATTATCCTGGACGATA
CGTTGACCCGCTGATGGGTCAGGTGAGAACCACGGAGTGGATGTCGGAACACATAGTCGATAATCAACAAGTGGAAGCAA
CGAGTGACGCAATGCGCTTAGCATCTGGTTACAGCTTTAACGTCAGTGACCACCCGCGTTCTGAGATTAACCGCGATTAC
CTCATGTTGTCTGTAATGCACACAGGTCAAGATCCGCAAGTGCATGAAGATGAAGCGAGTGGTATGCCAACCACTTACTA
CAACCAGTTCTCGTGTATTCCACGTGATGTGGTGTTTAAAGCGCCTAAATTAGCAGCACCAGTGGTTGATGGCCCTCAAA
CGGCGGTTGTTGTAGGACCAGAAGGTGAAGAGATTTACACTGACAAGCTTGGTCGAGTGAAAGTACAGTTCCATTGGGAT
CGCTACGGTGATAACAACGAGCATTCAAGCTGTTGGATTCGTGTAAGCCAATCCATGGCGGCGCCAACATGGGGCGCAGT
TTATTTGCCGCGTATTGGCCACGAAGTCGTAGTAACTTTCCTTGAGGGGGACCCAGATCGTCCGTTAGTTACGGGCGCAG
TTTACAACGGTTTGCATTTCCCACCGTACTCTTTGCCAGAAAACAAAACTCGTACCACGTTCCGCACGCAAACGCACAAA
GGAACAGGTTACAACGAGTTGAGTTTTGAAGACGAAGCGAACCAAGAAGAAGTCTATATTCACGCTCAAAAAGACATGTC
GACCAAGGTGCTCAACAACCGTTACCGTGATATCGGTCAAGATGAATTCTTGAAGGTTGCACGTCACCAAACGAATGAAG
TTCATGGTGACCATAAAGAAACCATTCATGGGCATAAAACGACACAGGTAAACAGTACGTTTACGGAGACAGTCGAGCAA
GACGTTACCGTCACATACAACGCGAACGAAGCCCAGTATGTGAAAAACAATTCTGACTTAGAAATTGGTGATAACCGTAC
GACTAAGATCGGTAAAAATGATGACTTAGATATTGGCGAAAACAGTAACCTTACTATCGGTGCGTCTAAGAGTTCAGATA
TTGGTGCGGATGATAACCAGACGATTGGTGGCAACTTAACGGTATCAGTGAAAGGGAACACCGCTTATAAAGCCGATGGT
GCAACGCAAATTATCAGTGGCGACAAGATTGTATTGAAAACGGGTGGCTCCTCTTTAGTGATGAACAGTGATGGTTCAAT
AAAGTTATCAGGCTCGGCTATTACGATCGAAGGCAGTGATAAAGTCGTCGTGAAAGGTGGCAACGTAGTGGTTAACTAA