Detailed information of component protein


Summary

Component ID T6CP012277
Component protein type FHA
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (425 a.a.); Nucleotide sequence (1275 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0004
Coordinate (Strand) 4297..5571 (-)
NCBI ID 225382147
GenBank FJ790776
Uniprot ID C0LZM2_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-424 MVNGNKILKNQPVAIVDGDIISLGQYEIGVSLEHVTSTLDIAADIAPERVSSDPLVSLGDAVVEEEEKVGALEDLFMETKQDDIDCHDPVAHLNFSMQRDDDYLIRDNERTESEQPRTVESTCQVVDDSFSIYSEFDQPSLIPEDWMAAETKSNASVNDALAPESGKAHRADIASAEVMQPGTVAPDSTIQRQHRTPNVTEMVVEQANTGSKWEEMTQSFTPSPNTNQQLQIPTAEHAINSTTKAQVDTDAAVCSQDIAQAFFDGLGIRNPELMTNEAQLFKQMGTCLRLCIDNLQKELHEVESLKDEQSASEANSNIAELMLTLNSQQLLAPNELVEQMLDELNDHQVMFNKALNELVLEQSETNDPVAFANRVASQSLFSTKSKLWAEYLDFYTNNRRLLNETSLKGLIKKNYNKVIKGNHA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9957 - -


  Protein sequence: 425 a.a.    

>T6CP012277 FJ790776:c5571-4297 [Vibrio alginolyticus] [FHA]
MVNGNKILKNQPVAIVDGDIISLGQYEIGVSLEHVTSTLDIAADIAPERVSSDPLVSLGDAVVEEEEKVGALEDLFMETK
QDDIDCHDPVAHLNFSMQRDDDYLIRDNERTESEQPRTVESTCQVVDDSFSIYSEFDQPSLIPEDWMAAETKSNASVNDA
LAPESGKAHRADIASAEVMQPGTVAPDSTIQRQHRTPNVTEMVVEQANTGSKWEEMTQSFTPSPNTNQQLQIPTAEHAIN
STTKAQVDTDAAVCSQDIAQAFFDGLGIRNPELMTNEAQLFKQMGTCLRLCIDNLQKELHEVESLKDEQSASEANSNIAE
LMLTLNSQQLLAPNELVEQMLDELNDHQVMFNKALNELVLEQSETNDPVAFANRVASQSLFSTKSKLWAEYLDFYTNNRR
LLNETSLKGLIKKNYNKVIKGNHA*

  Nucleotide sequence: 1275 bp    

>T6CP012277 FJ790776:c5571-4297 [Vibrio alginolyticus] [FHA]
ATGGTAAATGGGAATAAAATACTAAAAAATCAACCCGTTGCGATAGTTGATGGCGACATCATTTCTTTAGGGCAATATGA
GATTGGTGTTTCTCTTGAGCACGTTACCTCAACACTAGACATCGCCGCTGATATTGCTCCTGAGCGAGTATCTAGCGATC
CTCTGGTTAGTCTAGGAGATGCGGTTGTCGAAGAGGAAGAAAAAGTCGGTGCCTTAGAAGATTTGTTTATGGAGACTAAA
CAGGATGACATTGATTGTCATGATCCTGTTGCTCACCTTAACTTTTCGATGCAGCGAGATGACGATTACTTAATTCGTGA
TAATGAGCGTACAGAATCTGAACAACCTAGAACCGTGGAAAGCACGTGTCAGGTGGTCGATGACAGTTTCAGTATATATT
CCGAATTCGATCAGCCAAGCCTAATCCCTGAAGACTGGATGGCTGCGGAAACCAAGAGTAACGCATCGGTCAATGATGCA
TTAGCGCCAGAAAGCGGTAAGGCACATAGAGCCGATATTGCCTCAGCTGAGGTGATGCAACCTGGCACGGTAGCCCCAGA
TAGCACGATTCAAAGACAACATCGTACGCCTAATGTTACTGAGATGGTCGTAGAGCAGGCTAACACAGGGTCAAAATGGG
AAGAGATGACCCAATCTTTTACGCCTTCTCCTAACACTAATCAGCAACTTCAAATACCTACGGCAGAGCACGCGATAAAC
AGTACGACGAAAGCACAAGTCGATACTGATGCGGCCGTTTGCTCTCAAGATATAGCGCAAGCGTTTTTTGATGGTTTGGG
TATTCGAAATCCAGAGTTGATGACAAACGAGGCGCAGTTATTCAAGCAAATGGGCACTTGCTTGCGTTTATGTATTGATA
ACCTACAGAAAGAGTTGCACGAAGTGGAATCGTTAAAAGATGAGCAGAGTGCGAGTGAGGCTAACTCGAACATTGCAGAG
CTAATGCTCACTTTGAATAGTCAGCAATTACTGGCTCCAAATGAACTGGTTGAACAAATGCTCGATGAGCTGAACGATCA
CCAAGTTATGTTTAACAAAGCACTGAATGAGCTCGTTTTAGAACAGTCAGAAACGAACGATCCCGTAGCATTTGCTAACA
GGGTCGCTAGCCAGTCTTTGTTCTCAACTAAGTCAAAGCTGTGGGCCGAATACCTAGATTTCTACACTAATAATCGTCGC
CTGCTTAATGAAACATCCTTAAAAGGGCTGATTAAGAAAAACTATAACAAAGTGATTAAGGGAAACCATGCGTAG