Detailed information of component protein


Summary

Component ID T6CP010931
Component protein type TssE
T6SS ID (Type) T6SS00793 experimental (Type i1)
Strain Vibrio alginolyticus EPJS
Replicon chromosome
Sequence Protein sequence (137 a.a.); Nucleotide sequence (411 bp)
Reference
[1] Sheng L, Lv Y, Liu Q, et al. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA. Vet Microbiol. 2013 Mar 23;162(2-4):652-662. doi: 10.1016/j.vetmic.2012.09.009. Epub 2012 Sep 14. PMID: 23021863
[2] Sheng L, Gu D, Wang Q, et al. Quorum sensing and alternative sigma factor RpoN regulate type VI secretion system I (T6SSVA1) in fish pathogen Vibrio alginolyticus. Arch Microbiol. 2012 May;194(5):379-90. doi: 10.1007/s00203-011-0780-z. Epub 2011 Dec 16. PMID: 22173829
[3] Liu H, Gu D, Sheng L, et al. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Lett Appl Microbiol. 2012 Jul;55(1):73-81. doi: 10.1111/j.1472-765X.2012.03263.x. Epub 2012 May 25. PMID: 22563695
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VEPGS_0011
Coordinate (Strand) 15090..15500 (-)
NCBI ID 225382154
GenBank FJ790776
Uniprot ID C0LZM9_VIBAL
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
GPW_gp25 PF04965.16 5.7e-18 29..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-136 MEKGYRLLERIELGEPKNCYEKVVSHKHLIESIHSHLADLLNTHSGNAMIDNEYGLPDFNDVLSNNTNLVRHIQKNITSTIERFEPRLLNVEVHYREDHHNPLQLGFGIRGEVSHNGGKVPMSIDVYMGTDGQFNV


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9945 - -


  Protein sequence: 137 a.a.    

>T6CP010931 FJ790776:c15500-15090 [Vibrio alginolyticus] [TssE]
MEKGYRLLERIELGEPKNCYEKVVSHKHLIESIHSHLADLLNTHSGNAMIDNEYGLPDFNDVLSNNTNLVRHIQKNITST
IERFEPRLLNVEVHYREDHHNPLQLGFGIRGEVSHNGGKVPMSIDVYMGTDGQFNV*

  Nucleotide sequence: 411 bp    

>T6CP010931 FJ790776:c15500-15090 [Vibrio alginolyticus] [TssE]
ATGGAAAAAGGTTACCGTCTTTTAGAGCGGATCGAGCTAGGAGAACCAAAAAACTGCTACGAGAAAGTGGTGTCTCATAA
GCATTTGATTGAATCCATTCACTCGCATCTCGCTGACTTGTTGAATACGCATTCCGGCAACGCAATGATCGACAATGAGT
ATGGTCTGCCAGATTTTAACGACGTACTTTCTAACAACACGAACCTCGTTCGCCATATTCAAAAAAACATCACGTCAACC
ATAGAACGCTTTGAGCCCCGACTGCTGAATGTCGAGGTTCACTACCGTGAAGACCACCATAACCCATTACAACTCGGCTT
TGGTATTCGCGGCGAAGTTTCCCATAACGGCGGTAAAGTCCCAATGTCGATCGATGTCTACATGGGAACCGACGGCCAAT
TTAACGTTTAG